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Somatic Hybridization by Microfusion of Protoplasts

  • Chapter
Somatic Hybridization in Crop Improvement I

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 27))

Abstract

Somatic hybridization in plants has classically relied on en masse fusion of protoplasts. In general, large populations of protoplasts isolated from both parental forms are mixed and subjected to fusogenic treatments. Most fusion methods require the use of populations in the range of 106 plant cells per experiment. In protoplast fusion in a mixed population all possible combinations of fusants can be generated. In addition, since protoplast fusion frequencies are in the percentage range — depending on the fusogenic treatment used — unfused parental protoplasts will be present in such a mixture. Therefore, protoplast mass fusion allows for only limited, if any, control of the number and type of protoplasts participating in a certain fusion event. Thus, most experiments in plant cell hybridization, following this approach, require the use of selection procedures to enrich for the desired heteroplasmic fusion products out of the unfused parental cells and the homoplasmic fusants.

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Authors and Affiliations

  1. Institute for Plant Sciences, Swiss Federal Institute of Technology, ETH-Zentrum, Universitätstr. 2, 8092, Zürich, Switzerland

    G. Spangenberg

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  1. G. Spangenberg
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Editors and Affiliations

  1. A-137 New Friends Colony, 110065, New Delhi, India

    Y. P. S. Bajaj

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© 1994 Springer-Verlag Berlin Heidelberg

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Spangenberg, G. (1994). Somatic Hybridization by Microfusion of Protoplasts. In: Bajaj, Y.P.S. (eds) Somatic Hybridization in Crop Improvement I. Biotechnology in Agriculture and Forestry, vol 27. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57945-5_3

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  • DOI: https://doi.org/10.1007/978-3-642-57945-5_3

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-63411-6

  • Online ISBN: 978-3-642-57945-5

  • eBook Packages: Springer Book Archive

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