Abstract
Recent increase in the interest in secondary metabolite production has given new impetus to the use of cell genetic and molecular genetic approaches for metabolic engineering of medicinal plants. Although the exploitation of modern genetic technologies are still in their infancy, their potential has already been proved in the production of pharmaceutical compounds (Fett-Neto et al. 1992; Yun et al. 1992). Among the large variety of medicinal plants, the alkaloid-producing solanaceous species are particularly attractive candidates for cell/molecular genetic manipulations, primarily because tissue culture, including plant regeneration from protoplasts, has been optimized for many of these species. Therefore, it is not surprising that a great wealth of our knowledge on somatic hybridization and gene transformation derives from experiments with various medicinal plants of Solanaceae family.
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References
Bajaj YPS, Simola LK (1991) Atropa belladonna I: In vitro culture, regeneration of plants, cryopreservation and the production of tropane alkaloids. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 15. Medicinal and aromatic plants III. Springer, Berlin Heidelberg New York, pp 1–23
Bourgin JP (1986) Isolation and characterisation of mutant cell lines and plants: auxotrophs and conditional lethal mutants. In: Vasil IK (ed) Cell culture and somatic cell genetics of plants vol 3. Plant regeneration and genetic variability. Academic Press, New York, pp 475–512
Collin HA (1987) Determinants of yield of secondary products in plant tissue culture. Adv Bot Res 13: 145–187
Derks FHM, Hakkert JC, Verboek WHJ, Colign-Hooymans CM (1992) Genome composition of asymmetric hybrids in relation to the phylogenetic distance between the parents. Nucleus chloroplast interaction. Theor Appl Genet 84: 930–940
Endo T, Hamaguchi N, Eriksson T, Yamadaa Y (1991) Alkaloid biosynthesis in somatic hybrids of Duboisia leichhardtii F. Muell. and Nicotiana tabacum L. Planta 183: 505–510
Evans WC (1979) In: Hawkes JG, Lester RN, Skelding AD (eds) The biology and taxonomy of the Solanaceae. Academic Press, London, pp 241–254
Fett-Neto AG, DiCosmo F, Reynolds WF, Sakata K (1992) Cell culture of Taxus as a source of the antineoplastic drug taxol and related taxanes. Biotechnology 10: 1572–1576
Gebhardt C, Schnebli V, King PJ (1981) Isolation of biochemical mutants using haploid mesophyll protoplasts of Hyoscyamus muticus II. Planta 153: 81–89
Gebhardt C, Shimamoto K, Lazar G, Schnebli V, King PJ (1983) Isolation of biochemical mutants using haploid mesophyll protoplast of Hyoscyamus muticus III. Planta 159: 18–24
Gleba YY, Hinnisdaels S, Sidorov VA, Parokonny AS, Borysluk NV, Cherep NN, Negrutiu I, Jacobs M (1988) Intergeneric asymmetric hybrids between Nicotiana plumbaginifolia and Atropa belladonna obtained by “gamma-fusion”. Theor Appl Genet 76: 760–766
Glimelius K, Eriksson T, Grafe R, Muller A (1978) Somatic hybridisation of nitrate reductase-deficient mutants of Nicotiana tabacum by protoplast fusion. Physiol Plant 44: 273–277
Gray JC, Kung SD, Wildman SG, Sheen SJ (1974) Origin of Nicotiana tabacum detected by polypeptide composition of fraction I protein. Nature 252: 226–227
Harms CT (1983) Somatic hybridisation by plant protoplast fusion. In: Portrykus I, Harms CT, Hinnen A, Hutter R, King PJ, Shillito RD (eds) Protoplasts 1983, Experientia Suppl 46. Birkhäuser, Basel, pp 69–84
Heguaner R (1988) Biochemistry, distribution and taxonomic relevance of higher plant alkaloids. Phytochemistry 27: 2423–2427
Hinnisdaels S, Bariller L, Mouras A, Sidorov V, Del-Favero J, Venskens J, Negrutiu I, Jacobs M (1991) Highly asymmetric intergeneric nuclear hybrids between Nicotiana and Petunia: evidence for recombinogenic and translocation events in somatic hybrid plants after “gamma-fusion”. Theor Appl Genet 82: 609–614
Horsch RB, Fry JE, Hoffmann NL, Eizhholz D, Rogers SG, Fraley RT (1985) A simple and general method for transferring genes into plants. Science 227: 1229–1231
Imamura J, Saul MW, Potrykus I (1987) X-ray irradiation promoted asymmetric somatic hybridization and molecular analysis of the products. Theor Appl Genet 74: 445–450
Jia J, Potrykus I, Lazar GB, Saul M (1983) Hyoscyamus-Nicotiana fusion hybrids selected via auxotroph complementation and verified by species-specific DNA hybridization. In: Potrykus I, Harms CT, Hinnen A, Hutter R, King PJ, Shillito RD (eds) Protoplasts 1983. Birkhäuser, Basel, pp 110–111
Kostenyuk I, Lubaretz O, Borisyuk N, Voronin V, Stockigt J, Gleba Y (1991) Isolation and characterization of intergeneric somatic hybrids in the Apocynaceae family. Theor Appl Genet 82: 713–716
Krumbiegel G, Schieder O (1979) Selectionof somatic hybrids after fusion of protoplasts from Datura innoxia Mill. and Atropa belladonna L. Planta 145: 371–375
Kushnir S, Babiychuk E, Bannikova M, Momot V, Komarnitsky I, Cherep N, Gleba Y (1991) Nucleocytoplasmic incompatibility in cybrid plants possessing an Atropa genome and Nicotiana plastome. Mol Gen Genet 225: 225–230
Lazar G (1983) Recent development in plant protoplast fusion and selection technology. In: Potrykus I, Harms CT, Hinnen A, Hutter R, King PJ, Shillito RD (ed) Protoplasts 1983, Experientia Suppl 46. Birkhäuser, Basel, pp 61–67
Lazar G (1989) Somatic hybridization in Hyoscyamus × Nicotiana In: Bajaj YPS (eds) Biothechnology in agriculture and forestry, vol 8. Plant protoplasts and genetic engineering I. Springer, Berlin Heidelberg New York, pp 356–369
Lazar G, Fankhauser H, Potrykus I (1983) Complementation analysis of a nitrate reductase deficient Hyoscyamus muticus cell line by somatic hybridization. Mol Gen Genet 189: 359–364
Leete E (1979) Biosynthesis and metabolism of tropane alkaloids. Planta Med 36: 97–112
Lin GD, Griffin WJ (1992) Organogenesis and general procedure for plant regeneration from callus culture of a commercial Duboisia hybrid (D. leichhardtii × D. myoporoides). Plant Cell Rep 11: 207–210
Medgyesy P (1990) Selection and analysis of cytoplasmic hybrids, In: Dix PJ (ed) Plant cell line selection. VCH, Weinheim, pp 287–316
Müller AJ, Grafe R (1978) Isolation and characterisation of cell lines of Nicotiana tabacum lacking nitrate reductase. Mol Gen Genet 161: 67–76
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497
Nagata T, Takebe I (1971) Plating of isolated tobacco mesophyll protoplast on agar medium. Planta 99: 12–20
Nagy JI, Maliga P (1976) Callus induction and plant regeneration from mesophyll protoplast of Nicotiana sylvestris. Z Pflanzenphysiol 78: 453–455
Negrutiu I, Mousseau J (1980) Protoplast culture from in vitro-grown plants of Nicotiana sylvestris Spegg. and Comes. Z Pflanzenphysiol 100: 373–376
Negrutiu I, Hinnisdaels A, Mouras B, Gill BS, Gharti-Chhetri, Davey MR, Gleba YY, Sidorov V, Jacobs M (1989) Somatic versus sexual hybridization: features, facts, future. Acta Bot Neerl 38: 253–272
Piastuch WC, Bates GW (1990) Chromosomal analysis of Nicotiana asymmetric somatic hybrids by dot blotting and in situ hybridisation. Mol Gen Genet 222: 97–103
Potrykus I, Jia J, Lazar G, Saul M (1984) Hyoscyamus muticus + Nicotiana tabacum fusion hybrids selected via auxotroph complementation. Plant Cell Rep 3: 68–71
Sacristan MD, Gerdmann-Knorck M, Schieder O (1989) Incorporation of hygromycin resistance in Brassica nigra and its transfer to B. napus through asymmetric protoplast fusion. Theor Appl Genet 79: 194–200
Saul MV, Potrykus I (1984) Species-specific repetitive DNA used to identify interspecific somatic hybrids. Plant cell Rep 3: 65–67
Schieder O (1985) Genetic manipulation by protoplast fusion. In: Magnien E, De Nettancourt D (eds) Advances in agricultural biotechnology: genetic engineering of plants and microorganisms important for agriculture, Nijhoff/Junk, Dordrecht, pp 26–27
Shillito RD, Paszkowski J, Potrykus I (1983) Agarose plating and a bead-type culture technique enable and stimulate development of protoplast-derived colonies in a number of plant species. Plant Cell Rep 2: 244–247
Strauss A, Bucher F, King PJ (1987) Isolation of biochemical mutants using haploid mesophyll protoplasts of Hyoscyamus muticus. Planta 153: 75–80
Tepfer D (1990) Genetic transformation using Agrobacterium rhyzogenes. Physiol Plant 79: 140–146
Waller G, Nowacki FK (1978) Alkaloid biology and metabolism in plants. Plenum Press, New York, pp 121–141
Watts JM, Motoyoshi F, King JM (1974) Problems associated with the production of stable protoplasts of cells of tobacco mesophyll. Ann Bot 38: 667–671
Wernicke W, Thomas E (1980) Studies on morphogenesis from isolated plant protoplasts: shoot formation from mesophyll protoplasts of Hyoscyamus muticus and Nicotiana tabacum. Plant Sci Lett 17: 401–407
Wulff EV (1943) An introduction to historical plant geography. Cronica Botanica, Waltham, Mass, p32
Yun DJ, Hashimoto T, Yamada Y (1992) Metabolic engineering of medicinal plants: transgenic Atropa belladonna with improved alkaloid composition. Proc Natl Acad Sci USA 89:11799–11803
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Lazar, G. (1994). Somatic Hybridization of Medicinal Plants in the Family Solanaceae. In: Bajaj, Y.P.S. (eds) Somatic Hybridization in Crop Improvement I. Biotechnology in Agriculture and Forestry, vol 27. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57945-5_23
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DOI: https://doi.org/10.1007/978-3-642-57945-5_23
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