Cloning and Expression of Cell Cycle Genes in Schizosaccharomyces pombe
It is undoubtedly admitted that the study of the cell cycle and yeast genetics cannot be dissociated from each other. Both the fission yeast Schizosaccharomyces pombe and the budding yeast Saccharomyces cerevisiae are powerful genetic systems to investigate the life cycle in broad terms. In both organisms, a large collection of cell division cycle (cdc) mutants that arrest the cell cycle at a specific point have been isolated. Their characterization has contributed to the elucidation of the sequential events of the cell cycle and to the identification of genes whose activity is required for the progression through the cell cycle. The general principle is that the succession of each cell cycle step is dependent on the completion of the preceding step.
KeywordsVortex Recombination Dexamethasone Glucocorticoid Leucine
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