Cloning and Expression of Cell Cycle Genes in Schizosaccharomyces pombe

  • Guillaume Cottarel
Part of the Springer Lab Manual book series (SLM)


It is undoubtedly admitted that the study of the cell cycle and yeast genetics cannot be dissociated from each other. Both the fission yeast Schizosaccharomyces pombe and the budding yeast Saccharomyces cerevisiae are powerful genetic systems to investigate the life cycle in broad terms. In both organisms, a large collection of cell division cycle (cdc) mutants that arrest the cell cycle at a specific point have been isolated. Their characterization has contributed to the elucidation of the sequential events of the cell cycle and to the identification of genes whose activity is required for the progression through the cell cycle. The general principle is that the succession of each cell cycle step is dependent on the completion of the preceding step.


Minimal Medium Fission Yeast Schizosaccharomyces Pombe Cell Cycle Gene Restrictive Temperature 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Alfa C, Fantes P, Hyams J, McLeod M, Warbrick E (1993) Experiments with fission yeast: a laboratory course manual. Laboratory Press, Cold Spring HarborGoogle Scholar
  2. Allshire RC (1990) Introduction of large linear minichromosomes into Schizosaccharomyces pombe by an improved transformation procedure. Proc Natl Acad Sci USA, 87: 4043–4047PubMedCrossRefGoogle Scholar
  3. Apolinaro E, Nocero M, Jin M, Hoffman CS (1993) Cloning and manipulation of the Schizosaccharomyces pombe his7+ gene as a new selectable marker for molecular genetic studies. Curr Genet 24:491–495CrossRefGoogle Scholar
  4. Barbet N, Muriel WJ, Carr AM (1992) Versatile shuttle vectors and genomic libraries for use with Schizosaccharomyces pombe. Gene 114:59–66PubMedCrossRefGoogle Scholar
  5. Basi, G, Schmid E, Maundrell K (1993) TATA box mutations in the Schizosaccharomy ces pombe nmt1 promoter affect transcription efficiency but not the transcription start point or thiamine repressibility. Gene 123:131–136PubMedCrossRefGoogle Scholar
  6. Beach D, Nurse P (1981) High-frequency transformation of the fission yeast Schizosaccharomyces pombe. Nature (London) 290:140–142CrossRefGoogle Scholar
  7. Booher R, Beach D (1987) Interaction between cdc13 + and cdc2 + in the control of mitosis in fission yeast; dissociation of the G1 and G2 roles of the cdc2 + protein kinase. EMBO J 6:3441–3447PubMedGoogle Scholar
  8. Booher R, Beach D (1988) Involvement of cdc13 + in mitotic control in Schizosaccharomyces pombe: possible interaction of the gene product with microtubules. EMBO J 7: 2321–2327PubMedGoogle Scholar
  9. Bröker M (1987) Transformation of intact schizosaccharomyces pombe cells with plasmid DNA. BioTechniques7 5:516–518Google Scholar
  10. Bröker M (1993) Rapid transformation of cryopreserved competent Schizosaccharomyces pombe cells. BioFeedback 15:599–600Google Scholar
  11. Bröker M, Bαuml O (1989) New expression vectors for the fission yeast Schizosaccharomyces pombe. FEBS Letter 248:105–110CrossRefGoogle Scholar
  12. Cottarel G, Beach D, Deuschle U (1993) Two new multi-purpose multicopy Schizosaccharomyces pombe shuttle vectors, pSP1 and pSP2. Curr Genet 23:547–548PubMedCrossRefGoogle Scholar
  13. Cottarel G (1995) The Saccharomyces cerevisiae HIS3 and LYS2 genes complement the Schizosacchaformyces pombe his5-303 and lysl-131 mutations, respectively: new selectable markers and multi-purpose multicopy shuttle vectors pSP3 and pSP4. Curr Genet (in press)Google Scholar
  14. Ekwall K, Ruusala T (1991) Budding yeast CAN1 gene as a selection marker in fission yeast. Nucleic Acid Res 19:1150PubMedCrossRefGoogle Scholar
  15. Elble R (1992) A simple and efficient procedure for transformation of yeasts. BioTechniques 13:18–20PubMedGoogle Scholar
  16. Faryar K, Gatz C (1992) Construction of a tetracycline-inducible promoter in Schizosaccharomyces pombe. Curr Genet 21: 345–349PubMedCrossRefGoogle Scholar
  17. Forsburg SL (1993) Comparison of Schizosaccharomyces pombe expression system. Nucleic Acid Res 21:2955–2956PubMedCrossRefGoogle Scholar
  18. Hoffman CS (1993) Preparations of yeast DNA, RNA and proteins. In: Current protocols in molecular biology. Edited by Ausubel, Brent, Kingston, Moore, Seidman, Smith and Struhl.Google Scholar
  19. Hoffman CS, Winston F (1989) A transcriptionally regulated expression vector for the fission yeast Schizosaccharomyces pombe. Gene 84:473–479PubMedCrossRefGoogle Scholar
  20. Hood MT, Stachow C (1990) Transformation of Schizosaccharomyces pombe by electroporation. Nucleic Acid Res 18:688PubMedCrossRefGoogle Scholar
  21. Jones RH, Moreno S, Nurse P, Jones NC (1988) Expression of the SV40 promoter in fission yeast: identification and characterization of an AP-1 like factor. Cell 53:659–667PubMedCrossRefGoogle Scholar
  22. Kanter-Smoler G, Dahlkvist A, Sunnerhagen P (1994) Improved method for rapid transformation of intact Schizosaccharomyces pombe cells. BioTechniques 16:798–799PubMedGoogle Scholar
  23. Lee MG, Nurse P (1987) Complementation used to clone a human homologue of the fission yeast cell cycle control gene cdc2. Nature London 327:31–35PubMedCrossRefGoogle Scholar
  24. Lohka MJ, Hayes MK, Maller JL (1988) Purification of maturation-promoting factor, an essential regulator of early mitotic event. Proc Natl Acad Sci USA 85:3009–3013PubMedCrossRefGoogle Scholar
  25. Matsumoto T, Beach D (1991) Premature initiation of mitosis in yeast lacking RCC1 or an interacting GTPase. Cell 66:347–360PubMedCrossRefGoogle Scholar
  26. Maundrell K (1990) nmt1 of fission yeast a highly transcribed gene completely repressed by thiamine. J Biol Chem 265:10857–10864PubMedGoogle Scholar
  27. Maundrell K (1993) Thiamine-repressible expression vectors pREP and pRIP for fission yeast. Gene 123:127–130PubMedCrossRefGoogle Scholar
  28. McLeod M, Stein M, Beach, D (1987) The product of the mei3 + gene, expressed under the control of the mating-type locus, induces meiosis and sporulation in fission yeast. EMBO J 6:729–736PubMedGoogle Scholar
  29. Molz L, Booher R, Young P, Beach D (1989) cdc2 and the regulation of mitosis: six interacting mcs genes. Genetics 122: 773–782PubMedGoogle Scholar
  30. Moreno S, Klar A, Nurse P (1991) A guide to yeast genetics and molecular biology. Methods Enzymol 194:795–823PubMedCrossRefGoogle Scholar
  31. Nurse P (1975) Genetic control of cell size at cell division in yeast. Nature (London), 256:547–551CrossRefGoogle Scholar
  32. Okazaki K, Okazaki N, Kume K, Jinno S, Tanaka K, Okayama H (1990) High-frequency transformation method and library transducing vectors for cloning mammalian cDNAs by trans-complementation of Schizosaccharomyces pombe. Nucleic Acid Res 18: 6485–6489PubMedCrossRefGoogle Scholar
  33. Picard D, Schena M, Yamamoto KR (1990) An inducible expression vector for both fission and budding yeast. Gene 86:257–261PubMedCrossRefGoogle Scholar
  34. Prentice HL (1992) High efficiency transformation of Schizosaccharomyces pombe by electroporation. Nucleic Acid Res 20:621PubMedCrossRefGoogle Scholar
  35. Rusell P, Nurse P (1986) cdc25+ functions as an inducer in the mitotic control of fission yeast. Cell 45:145–153CrossRefGoogle Scholar
  36. Weilguny D, Praetorius M, Carr A, Egel R, Nielsen O (1991) New vectors in fission yeast: application for cloning the his2 gene. Gene 99:47–54PubMedCrossRefGoogle Scholar
  37. Xiong YT, Connolly B, Futcher B, Beach D (1991) Human D-type cyclin. Cell 65:691–699PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • Guillaume Cottarel
    • 1
  1. 1.MitotixCambridgeUSA

Personalised recommendations