Visualization on the Grid of Virus-Host Interaction
The visualization of macromolecular structures on voxels provides the basis for insights in dynamic events in assembly. Certain macromolecules arise as well organized frameworks. Symmetry or crystalline arrays arise through a strict geometry of subunits. Single asymmetric motifs can be superimposed on themselves taking such geometric constraints into account. Biological molecules are often composed of subunits arranged in a symmetrical manner Such conformations provide an opportunity for signal enhancement originating from the repetition of molecules in a larger configuration. Such repetitions require not only specific interactions among protein motifs, but also a built-in flexibility to accommodate conformational changes. Thus, it is advantageous to analyze critical molecular interactions with detailed protein folding information rooted in the volume density as a whole. Two-dimensional image projections can be collected in electron microscopes at temperatures of liquid nitrogen or helium. Using low-dose electron beams, viral assemblies of large lattices have been studied in vitreous ice to localize function-specific domains identified through difference imaging. Robust algorithms have been implemented based on various grid transformations to allow effective averaging of massive image data sets.
KeywordsSpatial Frequency Morphological Unit Insect Virus Projection Theorem Phase Refinement
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