A Fluorogenic PCR-Based Assay for the Rapid Detection of Salmonella
Salmonella isa Gram-negative bacterium that is capable of causing a wide range of enteric diseases, ranging from a self-limiting gastroenteritis with mild symptoms and of short duration, to a potentially life-threatening severe debilitating typhoid fever.Salmonellaare the most frequent cause of bacterial diarrhea in the United States and the number one cause of food-borne illness worldwide. S.enteritidisoften associated with foodborne outbreaks associated with raw or under-cooked eggs (Bean et al.,1990).
KeywordsMagnesium Albumin MgCl Diarrhea Boiling
Unable to display preview. Download preview PDF.
- Bean NH, Griffin PM (1990) Foodborne Disease Outbreaks in the United States, 19731987: Pathogens, Vehicles, and Trends. J Food Protect 53:804–817Google Scholar
- CDC Surveillance for Foodborne - disease outbreaks - United States, 1988–1992 MMWR 1996; 45:1988–1992Google Scholar
- Holland PM, Abramson RD, Watson R, Gelfand DH (1991).XXX. Detection of specific poly-merase chain reaction product by utilizing the 5’ to 3’ exonuclease activity of Therm us aquaticus DNA polymerase Proc Natl Acad Sci USA 88:7276–7280Google Scholar