A Fluorogenic PCR-Based Assay for the Rapid Detection of Salmonella

  • Paszko-Kolva Christine
  • Carl Yamashiro
Part of the Springer Lab Manuals book series (SLM)

Abstract

Salmonella isa Gram-negative bacterium that is capable of causing a wide range of enteric diseases, ranging from a self-limiting gastroenteritis with mild symptoms and of short duration, to a potentially life-threatening severe debilitating typhoid fever.Salmonellaare the most frequent cause of bacterial diarrhea in the United States and the number one cause of food-borne illness worldwide. S.enteritidisoften associated with foodborne outbreaks associated with raw or under-cooked eggs (Bean et al.,1990).

Keywords

Magnesium Albumin MgCl Diarrhea Boiling 

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References

  1. Bassler HA, Flood SJA, Livak KJ, Marmaro J, Knorr R, Batt CA (1995) The use of a fluorogenic probe in a PCR-based assay for the detection of Listeria monocytogenes. Appl Environ Microbiol 61:3724–3728PubMedGoogle Scholar
  2. Bean NH, Griffin PM (1990) Foodborne Disease Outbreaks in the United States, 19731987: Pathogens, Vehicles, and Trends. J Food Protect 53:804–817Google Scholar
  3. CDC Surveillance for Foodborne - disease outbreaks - United States, 1988–1992 MMWR 1996; 45:1988–1992Google Scholar
  4. Chalker RB, Blaser MJ (1988) A review of human salmonellosis III. Magnitude of Salmonella infection in the United States Rev of Infect Dis 10: 111–124CrossRefGoogle Scholar
  5. Chen S, Yee A, Griffiths M, Larkin C, Yamashiro CT, Behari R, Paszko-Kolva C, Rahn K, De Grandis SA (1997) The evaluation of a fluorogenic polymerase chain reaction assay for the detection of Salmonella species in food commodities. Intern J Food Micro 35: 239–250CrossRefGoogle Scholar
  6. Holland PM, Abramson RD, Watson R, Gelfand DH (1991).XXX. Detection of specific poly-merase chain reaction product by utilizing the 5’ to 3’ exonuclease activity of Therm us aquaticus DNA polymerase Proc Natl Acad Sci USA 88:7276–7280Google Scholar
  7. Lee LG. Connell CR, Bloch W 1993 Allelic discrimination by nick-translation PCR with fluorogenic probes. Nucleic Acids Res 21:3761–3766CrossRefGoogle Scholar
  8. Livak KJ, Flood SJA, Marmaro J, Giusti W, Deetz K 1993 Oligonucleotides with fluor-escent dyes at opposite ends provide a quenched probe system for detecting PCR product and nucleic acid hybridization. PCR Methods Appl 4:357–362CrossRefGoogle Scholar
  9. Witham P K, Yamashiro CT, Livak KJ, Batt CA (1996) A PCR-based assay for the detec-tion of Escherichia coli shiga-like toxin genes in ground beef. Appl Environ Microbiol 62:1347–1353PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • Paszko-Kolva Christine
    • 1
  • Carl Yamashiro
    • 2
  1. 1.Perkin-Elmer/Applied Biosystems Division, 850 Lincoln Centre Drive,Foster CityCA, 94404, USA, Accelerated Technology Laboratories IncUSA
  2. 2.Roche Molecular SystemsRoche Molecular SystemsAlamedaUSA

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