Advertisement

Virus Detection in Biopsy Specimens

  • Veijo Hukkanen
  • Pirkko Heino
Part of the Springer Lab Manuals book series (SLM)

Abstract

We describe nonradioactive in situ hybridization (ISH) approaches for localization of viral DNA and RNA sequences in infected tissue. The use of non-isotopic ISH methods has become common because they are now as sensitive as autoradiography but they do not have the the disadvantages of the isotopic methods, such as the long assay time and the requirement for special laboratory facilities (Musiani et al., 1994).

Keywords

Human Papilloma Virus Trigeminal Ganglion Acetic Anhydride Virus Detection Concentration Of200 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Egger D, Troxler M, Bienz K (1994) Light and electron microscopic in situ hybridization: non-radioactive labeling and detection, double hybridization, and combined hybridization-immunocytochemistry. J Histochem Cytochem 42:815–822PubMedCrossRefGoogle Scholar
  2. Erälinna J-P, Soilu-Hänninen M, Röyttä M, Hukkanen V, Salmi A, Salonen R (1996) Blood-brain barrier breakdown and increased intercellular adhesion molecule (ICAM-1/CD54) expression after Semliki Forest (A7) virus infection facilitates the development of experimental allergic encephalomyelitis. J Neuroimmunol 66:103–114PubMedCrossRefGoogle Scholar
  3. Furuta Y, Shinohara T, Sano K, Meguro M, Nagashima K (1990) In situ hybridisation with digoxigenin-labelled DNA probes for detection of viral genomes. J Clin Pathol 43:806–809PubMedCrossRefGoogle Scholar
  4. Heino P, Hukkanen V, Arstila P (1989) Detection of human papilloma virus (HPV) DNA in genital biopsy specimens by in situ hybridization with digoxigenin-labeled probes. J Virol Methods 26:331–338PubMedCrossRefGoogle Scholar
  5. Hukkanen V, Heino P, Sears A E, Roizman B (1990) Detection of herpes simplex virus latency-associated RNA in mouse trigeminal ganglia by in situ hybridization using nonradioactive digoxigenin-labeled DNA and RNA probes. Methods Mol Cell Biol 2:70–81Google Scholar
  6. Maples JA (1985) A method for the covalent attachment of cells to glass slides for use in immunohistochemical assays. Am J Clin Pathol 83:356–363PubMedGoogle Scholar
  7. McQuaid S, McMahon J, Allan GM (1995) A comparison of digoxigenin and biotin labelled DNA and RNA probes for in situ hybridization. Biotech Histochem 70:147–154PubMedCrossRefGoogle Scholar
  8. Morris RG, Arends MJ, Bishop PE, Sizer K, Duvall E, Bird CC (1990) Sensitivity of digoxigenin and biotin labelled probes for detection of human papillomavirus by in situ hybridisation. J Clin Pathol 43:800–805PubMedCrossRefGoogle Scholar
  9. Musiani G, Gentilomi G, Zerbini M, Gibellini D, Gallinella G, Pileri S, Baglioni P, La Placa M (1990) In situ detection of cytomegalovirus DNA in biopsies of AIDS patients using a hybrido-immunocytochemical assay. Histochemistry 94:21–25PubMedCrossRefGoogle Scholar
  10. Musiani M, Zerbini M, Gibellini D, Venturoli S, Gentilomi G, Gallinella G, La Placa M (1994) Viral diagnosis using hybridization assays with digoxigenin labeled probes. Clin Chim Acta 226:237–245PubMedCrossRefGoogle Scholar
  11. Permeen AMY, Sam CK, Pathmanathan R, Prasad U, Wolf H (1990) Detection of Epstein-Barr virus DNA in nasopharyngeal carcinoma using a non-radioactive digoxigeninlabelled probe. J Virol Methods 27:261–268PubMedCrossRefGoogle Scholar
  12. Ramakrishnan R, Levine M, Fink DJ (1994) PCR-based analysis of herpes simplex virus type 1 latency in the rat trigeminal ganglion established with a ribonucleotide reductase-deficient mutant. J Virol 68:7083–7091PubMedGoogle Scholar
  13. Wu T-C, Mann RB, Epstein JI, MacMahon E, Lee WA, Charache P, Hayward SD, Kurman RJ, Hayward GS, Ambinder RF (1991) Abundant expression of EBER1 small nuclear RNA in nasopharyngeal carcinoma. A morphologically distinctive target for detection of Epstein-Barr virus in formalin-fixed paraffin-embedded carcinoma specimens. Am J Pathol 138:1461–1469PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • Veijo Hukkanen
    • 1
  • Pirkko Heino
    • 2
  1. 1.University ofTurkuDepartment ofVirologyTurkuFinland
  2. 2.University ofWisconsin Medical SchoolMcArdle Laboratory for Cancer Research, 1400 University Ave., MadisonUSA

Personalised recommendations