Virus Detection in Biopsy Specimens
We describe nonradioactive in situ hybridization (ISH) approaches for localization of viral DNA and RNA sequences in infected tissue. The use of non-isotopic ISH methods has become common because they are now as sensitive as autoradiography but they do not have the the disadvantages of the isotopic methods, such as the long assay time and the requirement for special laboratory facilities (Musiani et al., 1994).
KeywordsHuman Papilloma Virus Trigeminal Ganglion Acetic Anhydride Virus Detection Concentration Of200
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- Erälinna J-P, Soilu-Hänninen M, Röyttä M, Hukkanen V, Salmi A, Salonen R (1996) Blood-brain barrier breakdown and increased intercellular adhesion molecule (ICAM-1/CD54) expression after Semliki Forest (A7) virus infection facilitates the development of experimental allergic encephalomyelitis. J Neuroimmunol 66:103–114PubMedCrossRefGoogle Scholar
- Hukkanen V, Heino P, Sears A E, Roizman B (1990) Detection of herpes simplex virus latency-associated RNA in mouse trigeminal ganglia by in situ hybridization using nonradioactive digoxigenin-labeled DNA and RNA probes. Methods Mol Cell Biol 2:70–81Google Scholar
- Wu T-C, Mann RB, Epstein JI, MacMahon E, Lee WA, Charache P, Hayward SD, Kurman RJ, Hayward GS, Ambinder RF (1991) Abundant expression of EBER1 small nuclear RNA in nasopharyngeal carcinoma. A morphologically distinctive target for detection of Epstein-Barr virus in formalin-fixed paraffin-embedded carcinoma specimens. Am J Pathol 138:1461–1469PubMedGoogle Scholar