PRINS: PrimedIn SituLabeling and Hybridization in One Step

  • JØrn Koch
Part of the Springer Lab Manuals book series (SLM)


PRINS (PRimed IN Situ labeling and hybridization in one step) is a technique superbly suited for the detection of tandemly repeated target sequences in chromosomes and nuclei (in situ) by oligonucleotide probes. Such sequences can be detected down to a target size of a few hundred bases with a simple short procedure rendering virtually 100% of potential targets labeled.


Myotonic Dystrophy Telomeric Repeat Chromosome Painting Roche Molecular Biochemical Cytogenet Cell 
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  1. Allshire RC, Dempster M, Hastie ND (1989) Human telomeres contain at least three types of G-rich repeat distributed non-randomly. Nucleic Acids Res 17:4611–4627PubMedCrossRefGoogle Scholar
  2. Cinti C, Santi S, Maraldi NM (1993) Localization of single copy gene by PRINS technique. Nucleic Acids Res 21:5799–5800PubMedCrossRefGoogle Scholar
  3. Fischer H, Koch J, Hindkjær J, Askholm H (1992) Primed in situ labelling (PRINS) and in situ hybridization. An optimization and comparison of two methods for fluorescent detection of nucleic acids in situ. Applied Fluorescence Technology 4:14–19Google Scholar
  4. Gall J, Pardue ML (1969) Formation and detection of RNA-DNA hybrid molecules in cytological preparations. Proc Natl Acad Sci USA 63:378–383PubMedCrossRefGoogle Scholar
  5. Hindkjær J, Brandt CA, Koch J, Lund TB, Kolvraa S, Bolund L (1995a) Simultaneous detection of centromere specific probes and chromosome painting libraries by a combination of PRimed IN Situ labeling and chromosome painting (PRINS-painting). Chromosome Research 3:41–44PubMedCrossRefGoogle Scholar
  6. Hindkjær J, Hammoudah S, Clausen N, Koch J, Pedersen B (1995b) Translocation (7;7;7) in a Child with Acute Lympho-blastic Leukemia Demonstrated with Conventional Cytogenetics, PRINS and Chromosome Painting. Cancer Genet Cytogenet 84:19–23PubMedCrossRefGoogle Scholar
  7. Hindkjær J, Hammoudah AFM, Hansen KB, Jensen PD, Koch J, Pedersen B (1995c) Translocation (1;16) identified by chromosome painting and PRimed IN Situ labelling (PRINS). Report of two cases and review of the cytogenetic literature. Cancer Genet Cytogenet 79:15–20PubMedCrossRefGoogle Scholar
  8. Hindkjær J, Koch J, Mogensen J, Pedersen S, Fischer H, Nygaard M, Junker S, Gregersen N, Kolvraa S, Bolund L (1991) In situ labelling of nucleic acids for gene mapping, diagnostics and functional cytogenetics. Biotech Forum Europe 12:752–756Google Scholar
  9. Kipling D, Mitchell AR, Masumoto H, Wilson HE, Nicol L, Cooke HJ (1995) CENP-B binds a novel centromeric sequence in the Asian mouse mus caroli. Mol Cell Biol 15:4009–4020PubMedGoogle Scholar
  10. Koch J, Hindkjær J, Kolvraa S, Bolund L (1995) Construction of a panel of chromosome specific oligonucleotide probes (PRINS-primers) useful for the identification of individual human chromosomes in situ. Cytogenet Cell Genet 71:142–147PubMedCrossRefGoogle Scholar
  11. Koch J, Kolvraa S, Gregersen N, Bolund L (1989) Oligonucleotide-priming methods for the chromosome-specific labelling of alpha satellite DNA in situ. Chromosoma 98:259–265PubMedCrossRefGoogle Scholar
  12. Krejci K, Koch J (1998) Improved detection and comparative sizing of human chromosomal telomers in situ. Chromosoma 107:198–203PubMedCrossRefGoogle Scholar
  13. Langer PR, Waldrop AA, Ward DC (1981) Enzymatic synthesis of biotin-labeled poly-nucleotides: Novel nucleic acid affinity probes. Proc Natl Acad Sci USA 78:6633–6637PubMedCrossRefGoogle Scholar
  14. Mitchell A, Jeppesen P, Hanratty D, Gosden J (1992) The organisation of repetitive DNA sequences on human chromosomes with respect to the kinetochore analysed using a combination of oligonucleotide primers and CREST anticentromere serum. Chromo-soma 101:333–341CrossRefGoogle Scholar
  15. Moyzis RK, Buckingham IM, Cram LS, Dani M, Deaven LL, Jones MD, Meyne J, Ratliff RL, Wu J-R (1988) A highly conserved repetitive DNA sequence, (TTAGGG),,, present at the telomeres of human chromosomes. Proc Natl Acad Sci USA 85:6622–6626PubMedCrossRefGoogle Scholar
  16. Pellestor F, Girardet A, Andréo B, Charlieu JP (1994) A polymorfic alpha satellite sequence specific for human chromosome 13 detected by oligonucleotide primed in situ labelling (PRINS). Hum Genet 94:346–348PubMedCrossRefGoogle Scholar
  17. Pellestor F, Girardet A, Lefort G, Andréo B, Charlieu JP (1995) Rapid in situ detection of chromosome 21 by PRINS technique. Am J Med Genet 56:1–8CrossRefGoogle Scholar
  18. Pinkel D, Straume T, Gray JW (1986) Cytogenetic analysis using quantitative, high-sen-sitivity, fluorescence hybridization. Proc Natl Acad Sci USA 83:2934–2938PubMedCrossRefGoogle Scholar
  19. Sriprakash KS, Lundh N, Huh M, Radding CM (1975) The specificity of 7v exonuclease. J Biol Chem 250:5438–5445PubMedGoogle Scholar
  20. Therkelsen AJ, Nielsen A, Koch J, Hindkjær J, Kolvraa S (1995) Staining of Human tel-omeres with primed in situ labeling (PRINS). Cytogenet Cell Genet 68:115–118PubMedCrossRefGoogle Scholar
  21. Therkelsen C, Koch J, Kolvraa S, Hindkjær J, Pedersen S, Bolund L (1993) Repeated primed in situ labeling: formation and labeling of specific DNA sequences in chromosomes and nuclei. Cytogenet Cell Genet 63:235–237CrossRefGoogle Scholar
  22. Wolf KW, Mitchell A, Nicol L, Jeppesen P (1994) Analysis of centromere structure in the fly megaselia scalaris (Phoridae, Diptera) using CREST sera, anti-histone antibodies, and a repetitive DNA probe. Biology of the Cell 80:11–23PubMedCrossRefGoogle Scholar

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© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • JØrn Koch
    • 1
  1. 1.Danich Cancer Society, Deptof Cytogenetics,rage Hansens Gade 2,Aarhus cDenmark

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