Abstract
The polymerase chain reaction (PCR), a method for amplifying nucleic acids (Saiki et al. 1985), has revolutionized molecular biology in many fields, not only in life science but also in diagnostic and routine applications. Since its invention in 1983 by Kary B. Mullis, more than 30.000 publications were issued dealing with improvements and additional applications. Besides other amplification techniques developed in the past (see Chapter 3035), PCR seems to be finally established in both research and diagnostic laboratory. Main reasons for this are its easy handling and a high degree of automation which facilitates standardization and routine work.
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© 2000 Springer-Verlag Berlin Heidelberg
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Haberhausen, G. (2000). PCR: Overview on Application Formats in Research and Clinical Diagnosis. In: Kessler, C. (eds) Nonradioactive Analysis of Biomolecules. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57206-7_26
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DOI: https://doi.org/10.1007/978-3-642-57206-7_26
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-64601-3
Online ISBN: 978-3-642-57206-7
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