Amplification of Nucleic Acids by Polymerase Chain Reaction: Overview on Principles and Applications

  • Arndt Rolfs
  • Ulrich Finckh
  • Peter Bauer
Part of the Springer Lab Manuals book series (SLM)

Abstract

The polymerase chain reaction (PCR) is a powerfulin vitromethod in molecular biology for selective, highly specific and exceptionally efficient amplification of nucleic acid sequences. In the 10 years since the first publication on PCR (Saiki et al., 1985) this method has grown to rival in popularity traditional microbiological, genetic and technical procedures for cloning, sequencing, gene detection and related procedures. Furthermore, in the meantime PCR and all of its different applications are rapid and convenient alternatives to traditional procedures such as blotting technologies, conventional hybridization and molecular cloning. Initially, PCR was a rather complex and tricky generic procedure applied to basic research problems in molecular biology. It has developed into a simple, multipurpose procedure more or less optimized for diverse applications in nearly every biological discipline and commercial area. There are frequent instances of PCR techniques having passed into the service laboratory environment. These service laboratories are providing a broad range of diagnostic tests mainly covering medical and forensic applications, but also environmental, agricultural and veterinary topics.

Keywords

Phenol Dopamine DMSO Titration Heparin 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • Arndt Rolfs
    • 1
  • Ulrich Finckh
    • 2
  • Peter Bauer
    • 3
  1. 1.Klinik für Neurologie und PolikliniMedizinische Fakultat,Zentrum für Nervenheilkunde,Gehsheimer StrGermany
  2. 2.Institut für HumangenetikUniversitätsklinikum EppendorfHamburgGermany
  3. 3.Institut fur HumangenetikUniversitatsklinikum EppendorfHamburgGermany

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