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Detection of Chlamydia pneumoniae DNA in white blood cells

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Chlamydia pneumoniae and Chronic Diseases
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Abstract

To diagnose C. pneumoniae, we have to use different strategies depending on the type of infection (acute or chronic). For diagnosing acute primary infection and acute re-infection useful diagnostic means are available. The best combination seems to be serology by MIF in combination with detection of C. pneumoniae in the respiratory tract by PCR and/or cell culture. Since C. pneumoniae can persist in the respiratory tract [1], serology can be used to distinguish between an acute and a persistent infection.

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References

  1. Hammerschlag MR, Chirgwin K, Roblin PM, Gelling M, Dumornay W, Mandel L, Smith P, Schachter J. (1992) Persistent infection with Chlamydia pneumoniae following acute respiratory illness. Clin Infect Dis 14: 178–182

    Article  PubMed  CAS  Google Scholar 

  2. Kuo CC, Shor A, Campbell LA, Fukushi H, Patton DL, Grayston JT. (1993) Demonstration of Chlamydia pneumoniae in atherosclerotic lesions of coronary arteries. J Infect Dis 167: 841–849

    Article  PubMed  CAS  Google Scholar 

  3. Maass M, Gieffers J, Krause E, Engel PM, Bartels C, Solbach W. (1998) Poor correlation between microimmunofluorescence serology and polymerase chain reaction for detection of vascular Chlamydia pneumoniae infection in coronary artery disease patients. Med Microbiol Immunol 187: 103–106

    Article  PubMed  CAS  Google Scholar 

  4. Boman J, Allard A, Persson K, Lundborg M, Juto P, Wadell G. (1997) Rapid diagnosis of respiratory Chlamydia pneumoniae infection by nested touchdown polymerase chain reaction compared with culture and antigen detection by EIA. J Infect Dis 175: 1523–1526

    Article  PubMed  CAS  Google Scholar 

  5. Condos R, McClune A, Rom WN, Schluger NW. (1996) Peripheral-blood-based PCR assay to identify patients with active pulmonary tuberculosis. Lancet 347: 1082–1085

    Article  PubMed  CAS  Google Scholar 

  6. Petersen E, Boman J, Persson K, Arnerlov C, Wadell G, Juto P, Eriksson A, Dahlen G, Angquist KA. (1998) Chlamydia pneumoniae in human abdominal aortic aneurysms. Eur J Vasc Endovasc Surg 15: 138–142

    Article  PubMed  CAS  Google Scholar 

  7. Boman J, Soderberg S, Forsberg J, Birgander LS, Allard A, Persson K, Jidell E, Kumlin U, Juto P, Waldenstrom A, Wadell G. (1998) High prevalence of Chlamydia pneumoniae DNA in peripheral blood mononuclear cells in patients with cardiovascular disease and in middleaged blood donors. J Infect Dis 178: 274–277

    Article  PubMed  CAS  Google Scholar 

  8. Saikku P, Leinonen M, Tenkanen L, Linnanmäki E, Ekman MR, Manninen V, Manttari M, Frick MH, Huttunen JK. (1992) Chronic Chlamydia pneumoniae infection as a risk factor for coronary heart disease in the Helsinki Heart Study. Ann Intern Med 116: 273–278

    PubMed  CAS  Google Scholar 

  9. Moazed TC, Kuo CC, Grayston JT, Campbell LA. (1998) Evidence of systemic dissemination of Chlamydia pneumoniae via macrophages in the mouse. J Infect Dis 177: 1322–1325

    Article  PubMed  CAS  Google Scholar 

  10. Maass M, Krause E, Kruger S, et al.. (1997) Coronary arteries harbor viable Chlamydia pneumoniae. 8th European Congress of Clinical Microbiology and Infectious Diseases, Lausanne, Switzerland, May 25-28, 1997, abstract 578

    Google Scholar 

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© 2000 Springer-Verlag Berlin Heidelberg

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Boman, J. (2000). Detection of Chlamydia pneumoniae DNA in white blood cells. In: L’age-Stehr, J. (eds) Chlamydia pneumoniae and Chronic Diseases. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57195-4_7

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  • DOI: https://doi.org/10.1007/978-3-642-57195-4_7

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-41136-9

  • Online ISBN: 978-3-642-57195-4

  • eBook Packages: Springer Book Archive

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