Chromosome Preparation — Polyamine Method

  • Derek Davies
  • Clare Hughes
Part of the Springer Lab Manuals book series (SLM)

Abstract

Conventional karyotyping involves cytogenetic analysis of a metaphase spread; flow cytometry can also be used to produce a flow karoytype from a monodispersed suspension of chromosomes, stained with two DNA dyes with different base pair specification. Bivariate analysis on a dual laser cytometer will separate individual chromosomes according to their differences in DNA content (size) and their base pair ratio. The only chromosomes which remain grouped are chromosomes 9–12, their DNA content and base pair ratios being too similar to be separated. Flow karyotypes have been produced for many types of human cells including fibroblasts, stimulated peripheral blood cells and lymphoblastoids cell lines. Other mammalian flow karyotypes have also been produced including rat, mouse, and hamster.

Keywords

EDTA Argon Citrate Syringe Trypsin 

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References

  1. 1.
    Green, DK. Analysing and sorting human chromosomes. Journal of Microscopy 1990; 159:237–245.PubMedCrossRefGoogle Scholar
  2. 2.
    Young, BD. Chromosome analysis by flow cytometry: a review. Bas Appl Histochem 1984; 28: 9–19.Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • Derek Davies
  • Clare Hughes

There are no affiliations available

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