Chromosome Preparation — Polyamine Method
Conventional karyotyping involves cytogenetic analysis of a metaphase spread; flow cytometry can also be used to produce a flow karoytype from a monodispersed suspension of chromosomes, stained with two DNA dyes with different base pair specification. Bivariate analysis on a dual laser cytometer will separate individual chromosomes according to their differences in DNA content (size) and their base pair ratio. The only chromosomes which remain grouped are chromosomes 9–12, their DNA content and base pair ratios being too similar to be separated. Flow karyotypes have been produced for many types of human cells including fibroblasts, stimulated peripheral blood cells and lymphoblastoids cell lines. Other mammalian flow karyotypes have also been produced including rat, mouse, and hamster.
KeywordsEDTA Argon Citrate Syringe Trypsin
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