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Preparation of Genomic DNA from Animal Cells

  • Stefan Surzycki
Part of the Springer Lab Manuals book series (SLM)

Abstract

This chapter describes three procedures for the isolation of anima DNA,one large scale procedure and two small scale procedures. All ofthese methods yield DNA suitable for restriction enzyme digestion, genomic library construction, Southern blot analysis and PCR. The large scale method will yield DNA in excess of 200 kb, whereas the small scale protocols will generate DNA of 50-150 kb.

Keywords

Microfuge Tube Recovery Procedure Conical Centrifuge Tube Scale Procedure Scale Protocol 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • Stefan Surzycki

There are no affiliations available

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