Cryotechniques

  • Geoffrey R. Newman
  • Jan A. Hobot
Part of the Springer Lab Manuals book series (SLM)

Abstract

Cryotechniques do not require tissue to be stabilised by fixatives prior to resin embedding, and so are useful for preserving antigenic sites that are sensitive to even low concentrations of fixative, or for preserving structures that cannot be stabilised during fixation or dehydration (Fixation Strategies, Sect. 1.1). The prerequisite for cryotechniques is for biological tissue to be rapidly frozen prior to any processing steps. Processing can then be in to resin (Cryosubstitution, Sect. 4.3; Freeze-Drying, Sect. 4.4), or the frozen tissue can be sectioned directly (Cryoultramicrotomy, Sect. 4.5).

Keywords

Cellulose Sucrose Acetone Epoxy Propane 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  • Geoffrey R. Newman
    • 1
  • Jan A. Hobot
    • 1
  1. 1.Medical Microscopy SciencesUniversity of Wales College of MedicineCardiffUK

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