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NMR and Immobilized Cells

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Immobilized Cells

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Abstract

Nuclear Magnetic Resonance (NMR) spectroscopy is based on the response of certain nuclei which possess an intrinsic magnetic moment (1H, 13C, 15N, 19F, 23Na, 31P, etc.) to an applied magnetic field. Such a technique can detect separate signals from various compounds within a sample and provide information on molecular identity and concentration. NMR is also widely recognized as a non-destructive, non-invasive technique for studying intracellular processes allowing in vivo estimation of intermediate metabolite concentrations in living systems. However, this is an insensitive non invasive method compared to most types of spectroscopy and requires very high cell densities for obtaining a good signal-to-noise ratio within a reasonable time.

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Author information

Authors and Affiliations

  1. UPRES-A CNRS 6022, Laboratoire de Génie Cellulaire, Université de Picardie Jules Verne, 33 rue Saint-Leu, Amiens, 80039, France

    Jean-Noël Barbotin & Jean-Charles Portais

  2. Instituto de Tecnologia Química e Biológica, (ITQB), Universidade Nova de Lisboa, Rua da Quinta Grande 6, Apartado 127, Oeiras, 2780-156, Portugal

    Paula M. Alves & Helena Santos

Authors
  1. Jean-Noël Barbotin
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  2. Jean-Charles Portais
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  3. Paula M. Alves
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  4. Helena Santos
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Editor information

Editors and Affiliations

  1. Food and Bioprocess Engineering Group, Wageningen University, P.O.B. 8129, 6700 EV, Wageningen, The Netherlands

    René H. Wijffels

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© 2001 Springer-Verlag Berlin Heidelberg

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Barbotin, JN., Portais, JC., Alves, P.M., Santos, H. (2001). NMR and Immobilized Cells. In: Wijffels, R.H. (eds) Immobilized Cells. Springer. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-56891-6_12

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  • DOI: https://doi.org/10.1007/978-3-642-56891-6_12

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-67070-4

  • Online ISBN: 978-3-642-56891-6

  • eBook Packages: Springer Book Archive

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