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Plant Biotechnology: Transgenic Crops for the Third Millennium

  • Frank Kempken
Chapter
Part of the Progress in Botany book series (BOTANY, volume 62)

Abstract

During recent years, the use of plant biotechnology and genetically modified (GM) crops has increased considerably. In 1999, transgenic soybean with engineered herbicide resistance accounted for more than 50% of the soybean grown in the US. Maize and cotton carrying an endotoxin gene from Bacillus thuringensis (Bt) exhibiting insect resistance are now widely used. A rice plant that produces enough β-carotene to supplement the daily requirements of a human and serves to enhance iron uptake was recently presented. Some of the most important milestones in the development of transgenic crops are summarized in Table 1. The data given there highlight the enormous developments that have occurred during the last two decades of this last millennium. Consequently, one may conclude that the future of GM crops during the first decade of the third millennium should be bright. However, recent years have also witnessed increasing anti-GM-crop activity. This is not only true in Europe; resistance is also growing notably overseas (Lehrman 1999), making the fate of transgenic crops somewhat uncertain. In most European countries, consumer acceptance of GM-based food products is low.

Keywords

Transgenic Plant Genetically Modify Male Sterility Genetically Modify Crop Transgenic Crop 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Ahl-Goy P, Duesing JH (1995) From pots to plots: genetically modified plants on trial. Biotech 13:54–58Google Scholar
  2. Barry GF, Taylor ML, Padgette SR, Kolac KH, Hallas LE, Della-Ciopa G, Kishore GM (1994) Cloning and expression in Escherichia coli of the glyphosate-to-aminomethyl-phosphonic acid degrading activity from Achromobacter sp. strain LBAA. Monsanto Technical Report MSLB13245. Monsanto, St. LouisGoogle Scholar
  3. Beachy RN (1999) Facing fear of biotechnology. Science 285:335PubMedCrossRefGoogle Scholar
  4. Beachy RN, Loesch-Fries S, Turner NE (1990) Coat protein-mediated resistance against virus infection. Annu Rev Phytopathol 28:451–474CrossRefGoogle Scholar
  5. Becker D, Brettschneider R, Lörz H (1994) Fertile transgenic wheat from microprojectile bombardment of scutellar tissue. Plant J 5:299–307PubMedCrossRefGoogle Scholar
  6. Bevan MW, Flavell RB, Chilton M-D (1983) A chimaeric antibiotic resistance gene as a selectable marker for plant cell transformation. Nature 304:184–187CrossRefGoogle Scholar
  7. Birch RG (1997) Plant transformation: problems and strategies for practical application. Annu Rev Plant Physiol Plant Mol Biol 48:297–326PubMedCrossRefGoogle Scholar
  8. Bock R, Hagemann R (2000) Extranuclear inheritance: plastid genetics: manipulation of plastid genomes and biotechnological applications. Prog Bot 61:76–90CrossRefGoogle Scholar
  9. Borisjuk NV, Borisjuk LG, Logendra S, Petersen F, Gleba Y, Raskin I (1999) Production of recombinant proteins in plant root exudates. Nat Biotechnol 17:466–469PubMedCrossRefGoogle Scholar
  10. Bowen B (1993) Markers for plant gene transfer. In: Kung S, Wu R (eds) Oral immunisation by transgenic plants. World Patent Application, pp 89–123Google Scholar
  11. Bytebier B, Deboeck F, De Greve H, Van Montagu M, Hernalsteens JP (1987) T-DNA organization in tumor cultures and transgenic plants of the monocotyedon Asparagus officinalis. Proc Natl Acad Sci USA 84:5345–5349PubMedCrossRefGoogle Scholar
  12. Canevascini S, Caderas D, Mandel T, Fleming AJ, Dupuis I, Kuhlmeier C (1996) Tissue-specific expression and promoter analysis of the tobacco Itp1 gene. Plant Physiol 112:513–524PubMedCrossRefGoogle Scholar
  13. Carrer H, Hockenberry TN, Svab Z, Maliga P (1993) Kanamycin resistance as a selectable marker for plastid transformation in tobacco. Mol Gen Genet 241:49–56PubMedCrossRefGoogle Scholar
  14. Cassas AM, Kononowicz AK, Zehr UB, Tomes DT, Axtell JD, Butler LG, Bressan RA, Hasegawa PM (1993) Transgenic sorghum plants via microprojectile bombardment. Proc Natl Acad Sci USA 90:11212–11216CrossRefGoogle Scholar
  15. Chan M, Chang H, Ho S, Tong W, Yu S (1993) Agrobacterium-mediated production of transgenic rice plants expressing a chimeric α-amylase promoter/β-glucuronidase gene. Plant Mol Biol 22:491–506Google Scholar
  16. Chappie C, Carpita N (1998) Plant cell walls as targets for biotechnology. Curr Opin Plant Biol 1:179–185CrossRefGoogle Scholar
  17. Chen L, Marmey P, Taylor NJ, Brizard JB, Espinza C, D’Cruz P, Huet H, Zhang S, De Kochko A, Beachy RN, Fauquet CM (1998) Expression and inheritance of multiple transgenes in rice plants. Nat Biotechnol 16:1060–1064PubMedCrossRefGoogle Scholar
  18. Chrispeels MJ (1991) Sorting of proteins in the secretory system. Annu Rev Plant Physiol Plant Mol Biol 42:35–49CrossRefGoogle Scholar
  19. Christou P, Ford TL, Kofron M (1991) Production of transgenic rice (Oryza sativa 1.) plants from agronomically important indica and japonica varieties via electric discharge particle acceleration of exogenous DNA into immature zygotic embryos. Biotech 9:957–962CrossRefGoogle Scholar
  20. Comai L, Fracciotti D, Hiatt WR, Thompson G, Rose RE, Stalker DM (1985) Expression in plants of a mutant aroA gene from Salmonella typhimurium confers tolerance to glyphosate. Nature 317:741–744CrossRefGoogle Scholar
  21. Conrad U, Fiedler U (1994) Expression of engineered antibodies in plant cells. Plant Mol Biol 26:1023–1030PubMedCrossRefGoogle Scholar
  22. Crow JF (1998) 90 years ago: the beginning of hybrid maize. Genetics 148:923–928PubMedGoogle Scholar
  23. Curtiss R, Cardineau GA (1990) World Intellectual Property Organization PCT/US89.03799Google Scholar
  24. Daniell H, Datta R, Varma S, Gray S, Lee S-B (1998) Containment of herbicide resistance through genetic engineering of the chloroplast genome. Nat Biotechnol 16:345–348PubMedCrossRefGoogle Scholar
  25. De Block M, Botterman J, Vandewiele M, Dockx J, Thoen C, Gosselé V, Movva NR, Thompson C, Van Monatgu M (1987) Engineering herbicide resistance in plants by expression of a detoxifying enzyme. EMBO J 6:2513–2518PubMedGoogle Scholar
  26. Delannay X, Bradely J, LaVallee BT, Proksh RK, Fuchs RL, Sims SR, Greenplate JT, Marrone PG, Dodson RB, Augustine JJ, Layton JG, Fischoff DA (1989) Field performance of transgenic tomato plants expressing the Bacillus thuringiensis var. kurstaki insect control protein. Biotech 7:1265–1269Google Scholar
  27. Della Penna D (1999) Nutritional genomics: manipulating plant micronutrients to improve human health. Science 285:375–379CrossRefGoogle Scholar
  28. Dempsey DA, Silva H, Klessig DF (1998) Engineering disease and pest resistance in plants. Trends Microbiol 6:54–61PubMedCrossRefGoogle Scholar
  29. De Neve M, De Loose M, Jacobs A, Van Houdt H, Kaluza B, Weidle U, Van Montagu M, Depicker A (1993) Assembly of an antibody and its derived antibody fragment in Nicotiana and Arabidopsis. Transgenic Res 2:227–237PubMedCrossRefGoogle Scholar
  30. Digeon JF, Guiderdoni E, Alary R, Michaux-Ferriere N, Joudrier P, Gautier MF (1999) Cloning of a wheat puroindoline gene promoter by IPCR and analysis of promoter regions required for tissue-specific expression in transgenic rice seeds. Plant Mol Biol 39:1101–1112PubMedCrossRefGoogle Scholar
  31. Duke SO (1996) Herbicide resistant crops. CRC, New YorkGoogle Scholar
  32. English JJ, Mueller E, Baulcombe DC (1996) Suppression of virus accumulation in transgenic plants exhibiting silencing of nuclear genes. Plant Cell 8:179–188PubMedGoogle Scholar
  33. Enserink M (1999) The Lancet scolded over Pusztai paper. Science 286:656PubMedCrossRefGoogle Scholar
  34. Ewen SW, Pusztai A (1999) Effect of diets containing genetically modified potatoes expressing Galanthus nivalis lectin on rat small intestine. Lancet 354:1353–1354PubMedCrossRefGoogle Scholar
  35. Faktor O, Kooter JM, Dixon RA (1996) Functional dissection of a bean chalcone synthase gene promoter in transgenic tobacco plants reveals sequence motifs essential for floral expression. Plant Mol Biol 32:849–859PubMedCrossRefGoogle Scholar
  36. Fiedler U, Phillips J, Atsaenko O, Conrad U (1997) Optimization of scFv antibody production in transgeneic plants. Immunotechnology 3:205–216PubMedCrossRefGoogle Scholar
  37. Finnegan J, McElroy D (1994) Transgene inactivation: plants fight back! Biotech 12:883–888CrossRefGoogle Scholar
  38. Fischhoff DA, Bowdish KS, Perlak FJ, Marrone PG, McCormick SM, Niedermeyer JG, Dean DA, Kusano-Kretzmer K, Mayer EJ, Rochester DE, Rogers SG, Fraley RT (1987) Insect tolerant transgenic tomato plants. Biotech 5:807–813CrossRefGoogle Scholar
  39. Fraley RT, Rogers SG, Horsch RB, Sanders PR, Flick JS, Adams SP, Bittner ML, Brand LA, Fink CL, Fry JS, Galluppi GR, Goldberg SB, Hoffman NL, Woo SC (1983) Expression of bacterial genes in plant cells. Proc Natl Acad Sci USA 80:4803–4807PubMedCrossRefGoogle Scholar
  40. Fromm ME, Taylor LP, Walbot V (1986) Stable transformation of maize after gene transfer by electroporation. Nature 319:791–793PubMedCrossRefGoogle Scholar
  41. Fuchs RL, Ream JE, Hammond BG, Naylor MW, Leimgrub RM, Berberich SA (1993) Safety assessment of the neomycine phosphotransferase II (NPTII) protein. Biotech 11:1543–1547CrossRefGoogle Scholar
  42. Gamborg OL, Phillips GC (1995) Plant cell, tissue and organ culture. Fundamental methods. Springer, Berlin Heidelberg New YorkCrossRefGoogle Scholar
  43. Gasser CS, Fraley RT (1989) Genetically engineering plants for crop improvement. Science 244:1293–1299PubMedCrossRefGoogle Scholar
  44. Gleave AP, Mitra DS, Mudge SR, Morris BA (1999) Selectable marker-free transgenic plants without sexual crossing: transient expression of cre recombinase and use of a conditional lethal dominant gene. Plant Mol Biol 40:223–235PubMedCrossRefGoogle Scholar
  45. Goldsbrough AP, Lastrella CN, Yoder JI (1993) Transposition mediated re-positioning and subsequent elimination of marker genes from transgenic potato. Biotech 11:1286–1292Google Scholar
  46. Gordon-Kamm WJ, Spencer TM, Mangano ML, Adams TR, Daines RJ, et al. (1990) Transformation of maize cells and regeneration of fertile transgenic plants. Plant Cell 2:603–618PubMedGoogle Scholar
  47. Graham MW, Craig S, Waterhouse PM (1997) Expression patterns of vascular-specific promoters RolC and Sh in transgenic potatoes and their use in engineering PLRV-resistant plants. Plant Mol Biol 33:729–735PubMedCrossRefGoogle Scholar
  48. Greiner S, Rausch T, Sonnewald U, Herbers K (1999) Ectopic expression of a tobacco invertase inhibitor homolog prevents cold-induced sweetening of potato tubers. Nat Biotechnol 17:708–711PubMedCrossRefGoogle Scholar
  49. Grusak MA (1999) Genomics-assisted plant improvement to benefit human nutrition and health. Trends Plant Science 4:164–166CrossRefGoogle Scholar
  50. Gupta HS, Pattanayak A (1993) Plant regeneration from mesophyll protoplasts of rice (Oryza sativa L.) Biotech 11:90–94CrossRefGoogle Scholar
  51. Gura T (1999) New genes boost rice nutrients. Science 285:994–995PubMedCrossRefGoogle Scholar
  52. Hagemann R (1992) Plastid genetics in higher plants. In: Herrman RG (ed) Cell organelles. Springer, Berlin Heidelberg New York, pp 65–96CrossRefGoogle Scholar
  53. Hain R, Stabel P, Czernilofski AP, Steinbiss HH, Herrera-Estrella L, Schell J (1985) Uptake, integration, expression and genetic transmission of a selectable chimeric gene by plant protoplasts. Mol Gen Genet 199:161–168CrossRefGoogle Scholar
  54. Hain R, Reif HJ, Krause E, Langebartels R, Kindl H, Vornam B, Wiese W, Schmelzer E, Schreier PH, Stocker RH, et al. (1993) Disease resistance results from foreign phytoalexin expression in a novel plant. Nature 361:153–156PubMedCrossRefGoogle Scholar
  55. Halfter U, Morris PC, Willmitzer L (1992) Gene targeting in Arabidopsis thaliana. Mol Gen Genet 231:186–193PubMedGoogle Scholar
  56. Hansen G, Wright MS (1999) Recent advances in the transformation of plants. Trends Plant Sci 4:226–231PubMedCrossRefGoogle Scholar
  57. Haq TA, Mason IIS, Clements JD, Aratzen CJ (1995) Oral immunization with a recombinant bacterial antigen produced in transgenic plants (comments). Science 268:714–716PubMedCrossRefGoogle Scholar
  58. Hernalsteens JP, Van Vliet F, Beuckeleer MD, Depicker A, Engler G, Lemmers M, Holsters M, Van Montagu M, Schell J (1980) The Agrobacterium tumefaciens Ti plasmid as a host vector system for introducing foreign DNA in plant cells. Nature 287:654–656CrossRefGoogle Scholar
  59. Herrera-Estrella L, Depicker A, Van Montagu M, Schell J (1983) Expression of chimaeric genes transferred into plant cells using a Ti-plasmid-derived vector. Nature 303:209–213CrossRefGoogle Scholar
  60. Hiatt AC, Cafferkey R, Bowdish K (1989) Production of antibodies in transgenic plants. Nature 342:76–78PubMedCrossRefGoogle Scholar
  61. Hobbs SLA, Kpodar P, Delong CMO (1990) The effect of T-DNA copy number, position and methylation on reporter gene expression in tobacco transformants. Plant Mol Biol 15:851–864PubMedCrossRefGoogle Scholar
  62. Hooykaas-Van Slogteren GMS, Hooykaas PJJ, Schilperoort RA (1984) Expression of Ti plasmid genes in monocotyledonous plants infected with Agrobacterium tumefaciens. Nature 311:763–64CrossRefGoogle Scholar
  63. Ingelbrecht I, Van Houdt H, Van Montague M, Depicker A (1994) Posttranscriptional silencing of reporter transgenes in tobacco correlates with DNA methylation. Proc Natl Acad Sci USA 91:10502–10506PubMedCrossRefGoogle Scholar
  64. Jach G, Gornhardt B, Mundy J, Logemann J, Pinsdorf E, Leah R, Schell J, Maas C (1995) Enhanced quantitative resistance against fungal disease by combinatorial expression of different barley antifungal proteins in transgenic tobacco. Plant J 8:97–109PubMedCrossRefGoogle Scholar
  65. Kempin SA, Liljegren SJ, Block LM, Rounsley SD, Yanofsky MF, Lam E (1997) Trageted disruption in Arabidopsis. Nature 389:802–803PubMedCrossRefGoogle Scholar
  66. Kempken F (1997) Biotechnology with plants — an overview. Prog Bot 58:428–440CrossRefGoogle Scholar
  67. Kempken F, Kempken R (2000) Transgene Pflanzen. Springer, Berlin Heidelberg New YorkGoogle Scholar
  68. Klein TM, Arentzen R, Lewis PA, Fitzpatrick-McElligott S (1992) Transformation of microbes, plants and animals by particle bombardment. Biotech 10:286–291CrossRefGoogle Scholar
  69. Knutzon DS, Thompson GA, Radke SE, Johnson WB, Knauf VC, Kridl JC (1992) Modification of Brassica seed oil by antisense expression of a stearoyl-acyl carrier protein desaturase gene. Proc Natl Acad Sci USA 89:2624–2628PubMedCrossRefGoogle Scholar
  70. Kosugi S, Suzuka I, Ohashi Y (1995) Two of the three promoter elements identified in a rice gene for proliferating cell nuclear antigen are essential for meristematic tissue-specific expression. Plant J 7:877–886PubMedCrossRefGoogle Scholar
  71. Koziel MG, Beland GL, Bowman C, Carozzi NB, Crenshaw R, et al. (1993) Field performance of elite transgenic maize plants expressing an insecticidal protein derived from Bacillus thuringiensis. Biotech 11:194–200CrossRefGoogle Scholar
  72. Krautwig B, Lörz H (1995) Perspektiven der Gentechnik in der Pflanzenzüchtung. Naturwissenschaften 48:219–224Google Scholar
  73. Kriete G, Niehaus K, Perlick AM, Puhler A, Broer I (1996) Male sterility in transgenic tobacco plants induced by tapetum-specific deacetylation of the externally applied non-toxic compound N-acetyl-L-phosphinothricin. Plant J 9:809–818PubMedCrossRefGoogle Scholar
  74. Kunkel T, Niu Q-W, Chan Y-S, Chua N-H (1999) Inducible isopentenyl transferase as a high-efficiency marker for plant transformation. Nat Biotechnol 17:916–919PubMedCrossRefGoogle Scholar
  75. Lehrman S (1999) GM backlash leaves US farmers wondering how to sell their crops. Nature 401:107CrossRefGoogle Scholar
  76. Li L, Qu R, de Kochko A, Fauquet C, Beachy RN (1993) An improved rice transformation system using the biolistic method. Plant Cell Rep 12:250–255CrossRefGoogle Scholar
  77. Linn F, Heidmann I, Saedler H, Meyer P (1990) Epigenic changes in the expression of the maize A1 gene in Petunia hybrida: a role of numbers of integrated gene copies and state of methylation. Mol Gen Genet 222:329–336PubMedCrossRefGoogle Scholar
  78. Loder N (1999a) Journal under attack over controversial paper on GM food. Nature 401:731Google Scholar
  79. Loder N (1999b) Royal Society: GM food hazard claim is “flawed”. Nature 399:188PubMedCrossRefGoogle Scholar
  80. Losey JE, Rayor LS, Carter ME (1999) Transgenic pollen harms monarch larvae. Nature 399:214PubMedCrossRefGoogle Scholar
  81. Ma JK, Vine ND (1999) Plant expression systems for the production of vaccines. Curr Top Microbiol Immunol 236:275–292PubMedCrossRefGoogle Scholar
  82. Ma JK, Lehner T, Stabila P, Fux CI, Hiatt A (1994) Assembly of monoclonal antibodies with IgG1 and IgA heavy chain domains in transgenic tobacco plants. Eur J Immunol 24:131–138PubMedCrossRefGoogle Scholar
  83. Ma JK, Hikmat BY, Wycoff K, Vine N, Chargelegue D, Yu L, Hein MB, Lehner T (1998) Charcterization of a recombinant plant monoclonal secretory antibody and preventive immunotherapy in humans. Nat Med 4:601–606PubMedCrossRefGoogle Scholar
  84. Mariani C, De Beuckeleer M, Truettner J, Leemans J, Goldberg RB (1990) Induction of male sterility in plants by a chimaeric ribonuclease gene. Nature 347:737–741CrossRefGoogle Scholar
  85. Mariani C, Gossele V, De Beuckeleer M, De Block M, Goldberg RB, De Greef W, Leemans J (1992) A chimaeric ribonuclease-inhibitor gene restores fertility to male sterile plants. Nature 357:384–387CrossRefGoogle Scholar
  86. Masood E (1999) Food scientist in GMO row defends “premature” warning. Nature 398:98PubMedCrossRefGoogle Scholar
  87. Matzke MA, Primig M, Trnovsky J, Matzke AJM (1989) Reversible methylation and inactivation of marker genes in sequentially transformed tobacco plants. EMBO J 8:643–649PubMedGoogle Scholar
  88. McBride KE, Svab Z, Schaaf DJ, Hogan PS, Stalker DM, Maliga P (1995) Amplification of a chimeric Bacillus gene in chloroplasts leads to an extraordinary level of an insecticidal protein in tobacco. Biotech 13:362–365CrossRefGoogle Scholar
  89. McGarvey PB, Hammond J, Dieneli MM, Hooper C, Fu Z-F, Dietzschold B, Koprowski H, Michaels PH (1995) Expression of rabies virus glycoprotein in transgenic tomatoes. Biotechnol 13:1484–1487CrossRefGoogle Scholar
  90. Meyer P, Niedenhof I, Ten Lohuis M (1994) Evidence for cytosine methylation of nonsymmetrical sequences in transgenic Petunia hybrida. EMBO J 13:2084–2088PubMedGoogle Scholar
  91. Monsanto (1997) Petition for determination of nonregulated status: Roundup Ready corn line GA21. Submitted to United States Department of Agriculture, Petition No. 97–099-01P. Monsanto, St. LouisGoogle Scholar
  92. Müller-Röber B, Sonnewald U, Willmitzer L (1992) Inhibition of the ADP-glucose pyro-phosphorylase in transgenic potatoes leads to sugar-storing tubers and influences tuber formation and expression of tuber storage protein genes. EMBO J11:1229–1238Google Scholar
  93. Müller-Röber B, La Cognata U, Sonnewald U, Willmitzer L (1994) A truncated version of an ADP-glucose pyrophosphorylase promoter from potato specifies guard cell-selective expression transgenic plants. Plant Cell 6:601–612PubMedGoogle Scholar
  94. Murry LE, Elliott LG, Capitant SA, West JA, Hanson KK, et al. (1993) Transgenic corn plants expressing MDMV strain B coat protein are resistant to mixed infections of maize dwarf mosaic virus and maize chlorotic mottle virus. Biotech 11:1559–1564CrossRefGoogle Scholar
  95. Napoli C, Lemieux C, Jorgensen R (1990) Introduction of a chimeric chalcone synthase gene into petunia results in reversible co-suppression of homologous genes in trans. Plant Cell 2:279–289PubMedGoogle Scholar
  96. Nawrath C, Poirier Y, Somerville C (1994) Targeting of the polyhydroxybutyrate biosyn-thetic pathway to the plastids of Arabidopsis thaliana results in high levels of polymer accumulation. Proc Natl Acad Sci USA 91:12760–12764PubMedCrossRefGoogle Scholar
  97. Nehra NS, Chibbar RN, Leung N, Caswell K, Mallard C, Steinhauer L, Baga M, Kartha KK (1994) Self-fertile transgenic wheat plants regenerated from isolated scutellar tissues following microprojectile bombardment with two distinct gene constructs. Plant J 5:285–297CrossRefGoogle Scholar
  98. Oakes JV, Shewmaker CK, Stalker DM (1991) Production of cyclodextrins, a novel carbohydrate, in the tubers of transgenic potato plants. Biotech 9:982–989CrossRefGoogle Scholar
  99. Owen MR, Gandecha A, Cockburn W, Whitelam GC (1992) Synthesis of a functional anti-phytochrome single chain Fv protein in transgenic tobacco. Biotech 10:790–794CrossRefGoogle Scholar
  100. Pang SZ, Slightom JL, Gonsalves D (1993) Different mechanisms protect transgenic tobacco against tomato spotted wilt and impatiens necrotic spot tospoviruses. Biotech 11:819–824CrossRefGoogle Scholar
  101. Park Y-D, Papp I, Moscone EA, Iglesias VA, Vaucheret H, Matzke AJM, Matzke MA (1996) Gene silencing mediated by promoter homology occurs at the level of transcription and results in meiotically heritable alterations in methylation and gene activity. Plant J 9:183–194PubMedCrossRefGoogle Scholar
  102. Poirier Y, Dennis DE, Klomparens K, Nawrath C, Somerville C (1992) Polyhydroxybutyrate, a biodegradable thermoplastic, produced in transgenic plants. Science 256:520–523PubMedCrossRefGoogle Scholar
  103. Poirier Y, Nawrath C, Somerville C (1995) Production of polyhydroxyalkanoates, a family of biodegradable plastics and elastomers, in bacteria and plants. Biotech 13:142–150CrossRefGoogle Scholar
  104. Potrykus I (1991) Gene transfer to plants: assessment of published approaches and results. Annu Rev Plant Physiol Plant Mol Biol 42:205–225CrossRefGoogle Scholar
  105. Powell Abel P, Nelson RE, De B, Hoffmann N, Rogers SG, Fraley RT, Beachy RN (1986) Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene. Science 232:738–743CrossRefGoogle Scholar
  106. Pröls F, Meyer P (1992) The methylation patterns of chromosomal integration regions influence gene activity of transferred DNA in Petunia hybrida. Plant J 2:465–475PubMedGoogle Scholar
  107. Raineri DM, Bottino P, Gordon MP, Nester EW (1990) Agrobacterium-mediated transformation of rice (Oryza sativa L.). Biotech 8:33–38Google Scholar
  108. Redenbaugh K, Hiatt W, Martineau B, Kramer M, Sheehy R, Sanders R, Houck R, Emlay D (1992) Safety assessment of genetically engineered fruits and vegetables: a case of the Flavr Savr tomato. CRC Press, Boca RatonGoogle Scholar
  109. Rhodes CA, Pierce DA, Mettler IJ, Mascarenhas D, Detmer JJ (1988) Genetically transformed maize plants from protoplasts. Science 240:204–207PubMedCrossRefGoogle Scholar
  110. Salmeron JM, Vernooij B (1998) Transgenic approaches to microbial disease resistance in crop plants. Curr Opin Plant Biol 1:347–352PubMedCrossRefGoogle Scholar
  111. Sanford JC, Johnston SA (1985) The concept of parasite-derived resistance: deriving resistance genes from the parasite’s own genome. J Theor Biol 113:395–405CrossRefGoogle Scholar
  112. Sanford JC, Klein TM, Wolf ED, Allen N (1987) Delivery of substance into cells and tissues using a particle bombardment process. J Part Sci Technol 5:27–37CrossRefGoogle Scholar
  113. Schäfer W, Görz A, Kahl G (1987) T-DNA integration and expression in a monocot crop plant after introduction of Agrobacterium. Nature 327:529–532CrossRefGoogle Scholar
  114. Schlösser E (1997) Allgemeine Phytopathologie, 2nd edn. Thieme, StuttgartGoogle Scholar
  115. Scholthof KBG, Scholthof HB, Jackson AO (1993) Control of plant virus diseases by pathogen-derived resistance in transgenic plants. Plant Physiol 102:7–12PubMedGoogle Scholar
  116. Schouten A (1998) Plantibodies. Requirements for expression and subcellular targeting. Ponsen and Looijen, WageningenGoogle Scholar
  117. Schouten A, Roosien J, Van Eengelen FA, De Jong GAM, Borst-Vrennsen AWM, Zilverenant JF, Bosch D, Stickema WJ, Gommers FJ, Schots A, Bakker J (1996) The C-terminal KDEL sequence increases the expression level of a single-chain antibody designed to be targeted to both the cytosol and the secretory pathway in transgenic tobacco. Plant Mol Biol 30:781–793PubMedCrossRefGoogle Scholar
  118. Sela-Buurlage MB, Ponstein AS, Bres-Vloemans SA, Melchers LS, Van den Elzen PJM, Cornelissen BJC (1993) Only specific tobacco (Nicotiana tabacum) chitinases and β-1,3-glucanases exhibit antifungal activity. Plant Physiol 101:857–863PubMedGoogle Scholar
  119. Shade RE, Schroeder HE, Pueyo JJ, Tabe LM, Murdock LL, Higgins TJV, Chrispeels MJ (1994) Transgenic pea seeds expressing the a-amylase inhibitor of the common bean are resistant to burchid beetles. Biotech 12:793–796CrossRefGoogle Scholar
  120. Shah DM, Horsch RB, Klee HJ, Kishore GM, Winter JA, Turner NE, Hironaka CM, Sanders PR, Gasser CS, Aykent S, Siegel NR, Rogers SG, Fraley RT (1986) Engineering herbicide tolerance in transgenic plants. Science 233:478–481PubMedCrossRefGoogle Scholar
  121. Shah DM, Rommens CM, Beachy RN (1995) Resistance to diseases and insects in transgenic plants: progress and applications to agriculture. Trends Biotechnol 13:362–368CrossRefGoogle Scholar
  122. Sheehy RE, Kramer M, Hiatt WR (1988) Reduction of polygalacturonase activity in tomato fruit by antisense RNA. Proc Natl Acad Sci USA 85:8805–8809PubMedCrossRefGoogle Scholar
  123. Shelton M, Roush RT (1999) False reports and the ears of men. Nat Biotechnol 17:832PubMedCrossRefGoogle Scholar
  124. Shillito RD, Saul MW, Paszkowski J, Müller M, Potrykus I (1985) High efficiency direct gene transfer to plants. Biotech 3:1099–1103CrossRefGoogle Scholar
  125. Slater S, Mitsky TA, Houmiel KL, Hao M, Reiser SE, Taylor NB, Tran M, Valentin HE, Rodriguez DJ, Stone DA, Padgette SR, Kishore G, Gruys KJ (1999) Metabolic engineering of Arabidopsis and Brassica for poly(3-hydroxybutyrate-co-3-hydroxyvalkerate) copolymer production. Nat Biotechnol 17:1011–1016PubMedCrossRefGoogle Scholar
  126. Smith CJS, Watson CF, Ray J, Bird CR, Morris PC, Schuch W, Grierson D (1988) Antisense RNA inhibition of polygalacturonase gene expression in transgenic tomatoes. Nature 334:724–726CrossRefGoogle Scholar
  127. Stam M, Mol JNM, Kooter JM (1997) The silence of genes in transgenic plants. Ann Bot 79:3–12CrossRefGoogle Scholar
  128. Stark DM, Timmermann KP, Barry GF, Preiss J, Kishore GM (1992) Regulation of the amount of starch in plant tissues by ADP glucose pyrophosphorylase. Science 258:287–292PubMedCrossRefGoogle Scholar
  129. Steinbüchel A, Füchtenbusch B (1998) Bacterial and other biological systems for polyester production. Trends Biotechnol 16:419–426PubMedCrossRefGoogle Scholar
  130. Stitt M, Sonnewald U (1995) Regulation of metabolism in transgenic plants. Annu Rev Plant Physiol Plant Mol Biol 46:341–368CrossRefGoogle Scholar
  131. Sukhapinda K, Kozuch ME, Rubin-Wilson B, Ainley WM, Merlo DJ (1993) Transformation of maize (Zea mays L.) protoplasts and regeneration of haploid transgenic plants. Plant Cell Rep 13:63–68CrossRefGoogle Scholar
  132. Tavladoraki P, Benvenuto E, Trinca S, De Martinis D, Cattaneo A, Galeffi P (1993) Transgenic plants expressing a functional single-chain Fv antibody are specifically protected from virus attack. Nature 366:469–472PubMedCrossRefGoogle Scholar
  133. Truve E, Aaspollu A, Honkanen J, Puska R, Mehto M, Hassi A, Teeri TH, Kelve M, Seppanen P, Saarma M (1993) Transgenic potato plants expressing mammalian 2′-5′ oligoadenylate synthetase are protected from potato virus X infection under field conditions. Biotech 11:1048–1052CrossRefGoogle Scholar
  134. Tsuchiya T, Toriyama K, Yoshikawa M, Ejiri S, Hinata K (1995) Tapetum-specific expression of the gene for an endo-ß-l,3-glucanase causes male sterility in transgenic tobacco. Plant Cell Physiol 36:487–494PubMedGoogle Scholar
  135. Twell D, Yamaguchi J, McCormick S (1990) Pollen-specific gene expression in transgenic plants: coordinate regulation of two different tomato gene promoters during microsporogenesis. Development 1109:705–713Google Scholar
  136. Uchimiya H, Iwata M, Nojiri C, Samarajeewa PK, Takamatsu S, Ooba S, Anzai H, Christensen AH, Quail PH, Toki S (1993) Bialaphos treatment of transgenic rice plants expressing a bar gene prevents infection by the sheath bight pathogen (Rhizoctonia solani). Biotech 11:835–856CrossRefGoogle Scholar
  137. United States Department of Agriculture (1999) http://www.econ.ag.gov/whatsnew/issues/biotech
  138. Vaeck M, Reynaerts A, Höfte H, Jansens S, Beuckeleer MD, Dean C, Zabeau M, Van Montagu M, Leemans J (1987) Transgenic plants protected from insect attack. Nature 328:33–37CrossRefGoogle Scholar
  139. Vasil IK (1988) Progress in the regeneration and genetic manipulation of cereal crops. Biotech 6:397CrossRefGoogle Scholar
  140. Vasil IK, Thorpe TA (1994) Plant cell and tissue culture. Kluwer, DortrechtGoogle Scholar
  141. Vasil V, Castillo AM, Fromm ME, Vasil IK (1992) Herbicide resistant fertile transgenic wheat plants obtained by microprojectile bombardment of regenerable embryonic callus. Biotech 10:667–674CrossRefGoogle Scholar
  142. Vasil V, Srivastava V, Castillo AM, Fromm ME., Vasil IK (1993) Rapid production of transgenic wheat plants by direct bombardment of cultured immature embryos. Biotech 11:1553–1558CrossRefGoogle Scholar
  143. Vauchert H (1994) Promoter-dependent trans-inactivation in transgenic tobacco plants: kinetic aspects of gene silencing and gene reactivation. C R Acad Sci III 317:310–323Google Scholar
  144. Voelker TA, Worrell AC, Anderson L, Bleibaum J, Fan C, Hawkins DJ, Radke SE, Davies HM (1992) Fatty acid biosynthesis redirected to medium chains in transgenic oilseed plants. Science 257:72–74PubMedCrossRefGoogle Scholar
  145. Wan Y, Lemaux PG (1994) Generation of large number of independently transformed fertile barley plants. Plant Physiol 104:37–48PubMedGoogle Scholar
  146. Weeks IT, Anderson OD, Blechl AE (1993) Rapid production of multiple independent lines of fertile transgenic wheat (Triticum aestivum). Plant Physiol 102:1077–1084PubMedGoogle Scholar
  147. Wilson FD, Flint HM, Deaton WR, Fischoff DA, Perlak FJ, Armstrong JA, Fuchs RL, Berberich SA, Parks NJ, Stapp VBR (1992) Resistance of cotton lines containing a Bacillus thuringiensis toxin to pink bollworm (Lepidoptera; Gelechiidae) and other insects. J Econ Entomol 85:1516–1521Google Scholar
  148. Ye X, Al-Babili S, Kloti A, Zhang J, Lucca P, Beyer P, Potrykus I (2000) Engineering the provitamin A (beta-carotene) biosynthetic pathway into (carotenoid-free) rice endosperm. Science 287:303–305PubMedCrossRefGoogle Scholar
  149. Yin Y, Chen L, Beachy RN (1997) Promoter elements required for phloem-specific gene expression from the RTBV promoter in rice. Plant J 12:1179–1188PubMedCrossRefGoogle Scholar
  150. Yoder JI, Goldsbrough AP (1994) Transformation systems for generating marker-free transgenic plants. Biotech 12:263–267CrossRefGoogle Scholar
  151. Zhu Q, Maher EA, Masoud S, Dixon RA, Lamb CJ (1994) Enhanced protection against fungal attack by constitutive co-expression of chitinase and glucanase genes in transgenic tobacco. Biotech 12:807–8812CrossRefGoogle Scholar
  152. Zoubenko OV, Allison LA, Svab Z, Maliga P (1994) Efficient targeting of foreign genes into the tobacco plastid genome. Nucleic Acids Res 22:3819–3824PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  • Frank Kempken
    • 1
  1. 1.Lehrstuhl für Allgemeine und Molekulare BotanikRuhr-Universität BochumBochumGermany

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