Hämodilution mit bovinem Hämoglobin (HBOC) vermindert den Ischämie/Reperfusionsschaden nach partiell warmer Leberischämie an der Ratte

Abstract

Background: Hemodilution is known to reduce ischemia/reperfusion injury. This is limited by the simultaneous reduction of the oxygen transport capacity. The aim of this study was to evaluate the effect of hemodilution with an oxygen carrying solution, HBOC, in comparison with colloidal and crystalloidal solutions on liver microcirculation in a model of partial warm liver ischemia. Methods: In four groups (seven male DA rats each) warm ischemia of the left liver lobe was performed for 70 min followed by 30 min of reperfusion. No hemodilution was carried out in the control group. The other groups received hemodilution with Ringer’s lactate, dextran or HBOC, respectively, to a hematocrit of 30% before the onset of ischemia. Intravital microscopic analysis was done after reperfusion with respect to sinusoidal perfusion, leukocyteendothelium interaction and blood cell velocity. Results: Intravital microscopic analysis showed no improvement in liver microcirculation after hemodilution with Ringer’s lactate. Microcirculation in the dextran group was significantly improved compared to control and Ringer’s lactate. A further improvement could be achieved by hemodilution with HBOC compared to all other groups (p < 0.05). Conclusion: Hemodilution with HBOC results in a significant reduction of ischemia/reperfusion injury compared to all other groups, even compared to dextran. This additional effect could be achieved by the oxygen transport capacity of HBOC in an oxygen-deprived tissue after ischemia.