Skip to main content
SpringerLink
Log in

cDNA Microarrays on Nylon Membranes with Enzyme Colorimetric Detection

  • Chapter
DNA Microarrays: Gene Expression Applications

Part of the book series: Principles and Practice ((PRINCIPLES))

Abstract

Enzyme colorimetric detection is an alternative to fluorescence detection that can be used when simultaneously measuring multiple parameters is required (Tijssen 1985; Thorpe and Kerr 1994). Colorimetric detection methods combined with the use of microarrays (microarray/CD) (Chen et al. 1998) make the already powerful microarray method (Schena et al. 1995; Chen et al. 1998; Iyer et al. 1999) even more useful to researchers. In this chapter, we describe how to use the enzyme colorimetric detection method in a microarray format using nylon membranes to simultaneously measure the expression level of multiple genes.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Benson DA, Boguski MS, Lipman DJ, Ostell J (1977) GenBank. Nucleic Acids Res 25:1–6

    Article  Google Scholar 

  • Bernard K, Auphan N, Granjeaud S, Victorero G, Schmitt-Verhulst AM, Jordan BR, Nguyen C (1996) Multiplex messenger assay: simultaneous, quantitative measurement of expression for many genes in the context of T cell activation. Nucleic Acids Res 24:1435–1442

    Article  PubMed  CAS  Google Scholar 

  • Bertucci F, Bernard K, Loriod B, Chang YC, Granjeaud S, Birnbaum D, Nguyen C, Peck K, Jordan BR (1999) Sensitivity issues in DNA array-based expression measurements and performance of nylon microarrays for small samples. Hum Mol Genet 8:1715–1722

    Article  PubMed  CAS  Google Scholar 

  • Bobrow MN, Harris TD, Shaughnessy KJ, Litt GJ (1989) Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays. J Immunol Methods 125:279–285

    Article  PubMed  CAS  Google Scholar 

  • Bobrow MN, Shaughnessy KJ, Litt GJ (1991) Catalyzed reporter deposition, a novel method of signal amplification. II. Application to membrane immunoassays. J Immunol Methods 137:103–112

    Article  PubMed  CAS  Google Scholar 

  • Boguski MS, Schuler GD (1995) Establishing a human transcript map. Nature Genet 10:369–371

    Article  PubMed  CAS  Google Scholar 

  • Braun BS, Freiden R, Lessnick SL, May WA, Denny CT (1995) Identification of target genes for the Ewing’s sarcoma EWS/FLI fusion protein by representational difference analysis. Mol Cell Biol 15:4623–4630

    PubMed  CAS  Google Scholar 

  • Cariaso M, Folta P, Lennon G, Wagner M, Kuczmarski T (1999) IMAGEne I: the clustering of ESTs corresponding to known genes. Bioinformatics 15:965–973

    Article  PubMed  CAS  Google Scholar 

  • Chen, JJ, Wu R, Yang PC, Huang JY, Sher YP, Han MH, Kao WC, Lee PJ, Chiu TF, Chang F, Chu YW, Wu CW, Peck K (1998) Profiling expression patterns and isolating differentially expressed genes by cDNA microarray system with colorimetry detection. Genomics 51(3):313–324

    Article  PubMed  CAS  Google Scholar 

  • Chu CC, Paul WE (1997) Fig1, an interleukin 4-induced mouse B cell gene isolated by cDNA

    Google Scholar 

  • representational difference analysis. Proc Natl Acad Sci USA 94:2507–2512

    Google Scholar 

  • DeRisi JL, Iyer VR, Brown PO (1997) Exploring the metabolic and genetic control of gene expression on a genomic scale. Science 278:680–686

    Article  PubMed  CAS  Google Scholar 

  • Eberwine J, Yeh H, Miyashiro K, Cao Y, Nair S, Finnell R, Zettel M, Coleman P (1992) Analysis of gene expression in single live neurons. Proc Natl Acad Sci USA 89:3010–3014

    Article  PubMed  CAS  Google Scholar 

  • Escribano J, Garcia-Carmona F, Iborra JL, Lozano JA (1984) Kinetic analysis of chemical reactions coupled to an enzymic step. Biochem J 223:633–638

    PubMed  CAS  Google Scholar 

  • Gossrau R (1978) Azoindoxyl methods for the investigation of hydrolases. IV. Suitability of various diazonium salts (author’s translation). Histochemistry 57:323–342

    Article  PubMed  CAS  Google Scholar 

  • Hellsing K, Richter W (1974) Immunochemical quantitation of dextran by a polymer enhanced

    Google Scholar 

  • nephelometric procedure. J Immunol Methods 5:147–151

    Google Scholar 

  • Hubank M, Schatz DG (1994) Identifying differences in mRNA expression by representational

    Google Scholar 

  • difference analysis of cDNA. Nucleic Acids Res 22:5640–5648

    Google Scholar 

  • Iyer VR, Eisen MB, Ross DT, Schuler G, Moore T, Lee JCF, Trent JM, Staudt LM, Hudson J Jr, Boguski MS, Lashkari D, Shalon D, Botstein D, Brown PO (1999) The transcriptional program in the response of human fibroblasts to serum. Science 283:83–87

    Article  PubMed  CAS  Google Scholar 

  • Kerr MA, Loomes LM, Thorpe SJ (1994) Enzyme-conjugated antibodies. In: Kerr MA, Thorpe R (eds) Immunochemistry LabFax. BIOS Scientific Publishers, Oxford, pp 149–152

    Google Scholar 

  • Lee N, Zhang SQ, Testa D (1988) A rapid multicolor Western blot. J Immunol Methods 106:27–30

    Article  PubMed  CAS  Google Scholar 

  • Lennon GG, Auffray C, Polymeropoulos M, Soares MB (1996) The IMAGE consortium: an integrated molecular analysis of genomes and their expression. Genomics 33:151–152

    Article  PubMed  CAS  Google Scholar 

  • Mathies RA, Peck K, Stryer L (1990) Optimization of high-sensitivity fluorescence detection. Anal Chem 62:1786–1791

    Article  PubMed  CAS  Google Scholar 

  • Nguyen C, Rocha D, Granjeaud S, Baldit M, Bernard K, Naquet P, Jordan BR (1995) Differential gene expression in the murine thymus assayed by quantitative hybridization of arrayed cDNA clones. Genomics 29:207–216

    Article  PubMed  CAS  Google Scholar 

  • Salunga RC, Guo H, Luo L, Bittner A, Joy KC, Chambers JR, Wan JS, Jackson MR, Erlander MG (1999) Gene expression analysis via cDNA microarrays of laser capture microdissected cells from fixed tissue. In: Schena M (ed) DNA microarrays. A practical approach. Oxford University Press, Oxford, pp 121–137

    Google Scholar 

  • Schena M, Shalon D, Davis RW, Brown PO (1995) Quantitative monitoring of gene expression patterns with a complementary DNA microarray. Science 270:467–470

    Article  PubMed  CAS  Google Scholar 

  • Schuler GD, Boguski MS, Stewart EA, Stein LD, Gyapay G, Rice K, White RE, Rodrigues-Tome P, Aggarwal A, Barjorek E et al. (1996) A gene map of the human genome. Science 274:540–546

    Article  PubMed  CAS  Google Scholar 

  • Thorpe SJ, Kerr MA (1994) Common immunological techniques. In: Kerr MA, Thorpe R (eds)

    Google Scholar 

  • Immunochemistry LabFax. BIOS Scientific Publishers, Oxford, pp 198–204

    Google Scholar 

  • Tijssen P (1985) Practice and theory of enzyme immunoassays, Elsevier, Amsterdam

    Google Scholar 

  • Van Gelder RN, von Zastrow ME, Yool A, Dement WC, Barchas JD, Eberwine JH (1990) Amplified RNA synthesized from limited quantities of heterogeneous cDNA. Proc Natl Acad Sci USA 87:1663–1667

    Article  PubMed  Google Scholar 

  • Zaitsu K, Ohkura Y (1980) New fluorogenic substrates for horseradish peroxidase: rapid and sensitive assays for hydrogen peroxide and the peroxidase. Anal Biochem 109:109–113

    Article  PubMed  CAS  Google Scholar 

  • Zhang L, Zhou W, Velculescu VE, Kern SE, Hruban RH, Hamilton SR, Vogelstein B, Kinzler KW (1997) Gene expression profiles in normal and cancer cells. Science 276:1268–1272

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Biomedical Sciences, Academia Sinica, 128 Sec. 2, Yen Chiu Yuan Road, Taipei, Taiwan 115, ROC

    Konan Peck & Yuh-Pyng Sher

Authors
  1. Konan Peck
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yuh-Pyng Sher
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Parc Scientifique de Luminy, Case 901, Marseille-Génopole, 13288, Marseille, Cedex 9, France

    B. R. Jordan

Rights and permissions

Reprints and permissions

Copyright information

© 2001 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Peck, K., Sher, YP. (2001). cDNA Microarrays on Nylon Membranes with Enzyme Colorimetric Detection. In: Jordan, B.R. (eds) DNA Microarrays: Gene Expression Applications. Principles and Practice. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-56517-5_3

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-56517-5_3

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-41508-4

  • Online ISBN: 978-3-642-56517-5

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation