Simultaneous Multicolor-FISH and Immunocyto-chemical Analysis of Fresh Tumor Material

  • Heike Steilen-Gimbel
  • Steffi Urbschat
Part of the Springer Lab Manuals book series (SLM)


A variety of immuncytochemical (IC) and in situ hybridization (ISH) methods give insight into the presence or absence of specific proteins and nucleic acid sequences, or establish kinetic parameters of cells. The combination of these techniques in the same biological specimen has proven advantageous in, e.g., simultaneously demonstrating the presence of mRNA and its protein product in a given cell (Dirks et al. 1993; Trembleau et al. 1993), immunophenotyping cells containing a specific chromosomal aberration (Gendelman et al. 1985; Porter et al. 1990; Van den Berg et al. 1991; Weber-Matthiesen et al. 1993), or characterization of cytokinetic parameters of tumor cell populations that are genetically or phenotypically aberrant (Balazs et al. 1991; Van Dekken et al. 1991).


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Copyright information

© Springer-Verlag Berlin Heidelberg 2002

Authors and Affiliations

  • Heike Steilen-Gimbel
    • 1
  • Steffi Urbschat
    • 2
  1. 1.Department of Neurosurgery, Institute of Human GeneticsSaarland UniversityHomburg / SaarGermany
  2. 2.Institute of Human GeneticsSaarland UniversityHomburg / SaarGermany

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