Abstract
Peripheral blood is the most frequently used tissue for chromosome studies, as it is easy to obtain, can simply be brought into short-term culture and metaphase spreads can be prepared in high quality within a short time. Such metaphase spreads, as well as the huge amounts of previously superfluous interphase nuclei in cytogenetic preparations, are a material very well suited to FISH analyses. All kinds of FISH probes can be used to analyze metaphase spreads, while the interphase nuclei can actually be analyzed successfully only by satellite and (sub-)telomere probes, plasmids, cosmids, BACs, YACs or PI-clones and not — at least not in routine-approaches — by whole or partial chromosome painting probes. However, the quality of metaphases is important in routine diagnostics, as well as in research approaches (e.g. gene mapping). Thus, the preparation procedure of the chromosomes themselves is discussed in more detail in the following.
Keywords
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Claussen, U, Lemke, J, Michel, S, Chudoba, I, Mühlig, P, Westermann, M, Claussen, J, Rubtsov, N, Grummt U-W (1999) The concept of chromosome condensation and de-condensation during mitosis has to be replaced by the concept of chromosome region specific swelling. Cytogenet Cell Genet 1999; Vol 85: p 166
Ford, CE, Hamerton, JL (1956) Ford, CE, Hamerton, JL (1956) The chromosomes in man. Nature 178:1020–1030
Heng, HHQ, Tsui L-C (1993) Modes of DAPI banding and simultaneous in situ hybridization. Chromosoma, 102:325–332
Hliscs, R, Mühlig, P, Claussen, U (1997) The spreading of metaphases is a slow process which leads to a stretching of chromosomes. Cytogenet Cell Genet, 76:167–171
Hsu TC (1952) Mammalian chromosomes in vitro. 1. karyotype of man. J of Heredity, 43:167–172
Hughes A (1952) Some effects of abnormal toxicity on dividing cells in chick tissue cultures. Quart J of Mikroscop Sci, 93:207
Moorhead, PS, Nowell, PC, Mellman, WJ (1960) Chromosome preparations of leukocytes cultured from human peripheral blood. Exp Cell Res 20: 613–616
Nietzel A, Rocchi M, Heller A, Starke H, Wlodarska I, Beensen V, Claussen U, Liehr 7 (2001) A new multicolor-FISH approach for the characterization of marker chromosomes: Centromere-specific multicolor-FISH (cenM-FISH). Hum Genet, 108: 199–204
Nowell, PC (1960) Phytohemagglutinin: An initiator of mitosis in cultures of normal human leukocytes. Cancer Res 20:462–466
Senger, G, Chudoba, I, Plesch A (1998) Multicolor-FISH - the identification of chromosome aberrations by 24 colors. BlOforum, 9/98: 99–503
Spurbeck, JL, Zinsmeister, AR, Meyer, KJ, Jatal, SM (1996) Dynamics of chromosome spreading. Am J Med Genet, 61:387–393
Tjio, JH, Levan, A (1956) The chromosome number of man. Hereditas, 42:6–9
Wegner, RD (1999) Diagnostic Cytogenetics. Springer, Berlin Heidelberg
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2002 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Liehr, T., Claussen, U. (2002). FISH on Chromosome Preparations of Peripheral Blood. In: Rautenstrauss, B.W., Liehr, T. (eds) FISH Technology. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-56404-8_3
Download citation
DOI: https://doi.org/10.1007/978-3-642-56404-8_3
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-47739-3
Online ISBN: 978-3-642-56404-8
eBook Packages: Springer Book Archive