FISH on Chromosome Preparations of Peripheral Blood
Peripheral blood is the most frequently used tissue for chromosome studies, as it is easy to obtain, can simply be brought into short-term culture and metaphase spreads can be prepared in high quality within a short time. Such metaphase spreads, as well as the huge amounts of previously superfluous interphase nuclei in cytogenetic preparations, are a material very well suited to FISH analyses. All kinds of FISH probes can be used to analyze metaphase spreads, while the interphase nuclei can actually be analyzed successfully only by satellite and (sub-)telomere probes, plasmids, cosmids, BACs, YACs or PI-clones and not — at least not in routine-approaches — by whole or partial chromosome painting probes. However, the quality of metaphases is important in routine diagnostics, as well as in research approaches (e.g. gene mapping). Thus, the preparation procedure of the chromosomes themselves is discussed in more detail in the following.
KeywordsVortex Formaldehyde Toxicity Dust EDTA
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