Abstract
Peptide nucleic acids (PNAs) are nucleic acid mimics that contain a pseudo-peptide backbone composed of charge neutral and achiral N-(2-aminoethyl) glycine units to which the nucleotides are attached via a methylene carbonyl linker (Nielsen et al. 1991; Egholm et al. 1992; Egholm et al. 1993). PNAs hybridize with high affinity to complementary DNA sequences, forming PNA-DNA complexes via Watson-Crick or Hoogsteen binding (Leijon et al. 1994). The PNA/DNA duplex has a higher melting temperature, Tm, than the DNA/DNA duplex. For instance, a typical 15-mer PNA/DNA melts at 69°C whereas the corresponding DNA/DNA duplex melts at 54°C (Egholm et al. 1993). In addition to the high thermal stability of complexes, PNA-DNA binding is highly sensitive to mismatches (Nielsen et al. 1993; Egholm et al. 1993; Jensen et al. 1997). The Tm changes caused by single base mismatch averaged 15 and 11 °C, respectively, in PNA/DNA and DNA/DNA duplexes for a 15-mer oligo. These novel characteristics of short PNA oligomers obviously lead to development of PNA fluorescence in situ hybridization (PNA-FISH).
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© 2002 Springer-Verlag Berlin Heidelberg
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Strauss, W.M. (2002). PNA-FISH. In: Rautenstrauss, B.W., Liehr, T. (eds) FISH Technology. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-56404-8_19
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DOI: https://doi.org/10.1007/978-3-642-56404-8_19
Publisher Name: Springer, Berlin, Heidelberg
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