Combined in Situ Nick Translation and mRNA FISH for Simultaneous Detection of Apoptosis and Expression of Apoptosis-Related Genes in Sections from Paraffin-Embedded Tissue

  • Norbert Heckmann
Part of the Springer Lab Manuals book series (SLM)


Simultaneous demonstration of apoptotic cells and apoptosis-related gene expression on the same tissue section correlated to histomorphological features, appears to be of great interest.


Colorectal Adenoma Hyperplastic Polyp Simultaneous Demonstration Hybridization Mixture Dextran Sulphate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bosari S, Moneghini L, Graziani D, Lee AK, Murray JJ, Coggi G, Viale G (1995) Bcl-2 oncoprotein in colorectal hyperplastic polyps, adenomas, and adenocarcinomas. Hum-Pathol 26(5): 534–540PubMedCrossRefGoogle Scholar
  2. Hell K, Lorenzen J, Fischer R, Hausmann ML (1995) Hodgkin cells accumulate mRNA for bcl-2. Laboratory Investigation 73(4):492PubMedGoogle Scholar
  3. Kikuchi Y, Dinjens WN, Bosman FT (1997) Proliferation and apoptosis in proliferative lesions of the colon and rectum. Virchows-Arch. 431(2): 111–117PubMedCrossRefGoogle Scholar
  4. Mosnier JF, Perret AG, Vindimian M, Dumollard JM, Balique JG, Perpoint B, Boucheron S (1996) An immunohistochemical study of the simultaneous expression of bcl-2 and p53 oncoproteins in epithelial tumors of the colon and rectum. Arch-Pathol-Lab-Med. 120(7): 654–659PubMedGoogle Scholar
  5. Mueller J, Mueller E, Hoepner I, Jutting J, Bethke B, Stolte M, Hofier H (1996) Expression of bcl-2 and p53 in de novo and ex-adenoma colon carcinoma: a comparative immunohistochemical study. J-Pathol. 180(3): 259–265PubMedCrossRefGoogle Scholar
  6. Watson AJ, Merritt AJ, Jones LS, Askew JN, Anderson E, Becciolini A, Balzi M, Potten CS, Hickman JA (1996) Evidence of reciprocity of bcl-2 and p53 expression in human colorectal adenomas and carcinomas. Br-J-Cancer. 73(8): 889–895PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2002

Authors and Affiliations

  • Norbert Heckmann
    • 1
  1. 1.Institute of Anatomy and Cell BiologySaarland UniversityHomburgGermany

Personalised recommendations