Abstract
The major histocompatibility complex (MHC) has been the most intensively studied region of the human genome during the past three decades (Bodmer 1995) and it has also been widely studied in other mammalian species, including primates (Trowsdale 1995). These studies suggest that the MHC is probably the area of the mammalian genome that is most densely populated with functional genes. In humans, the MHCis principally known as the region that includes genes encoding the human leukocyte antigens (HLA), which playa crucial role in regulating the immune response in health and disease. These HLA genes are the most polymorphic found in humans and, since most polymorphisms encode functional variants, this polymorphism results in inter-individual variation in the immune response. As a result of this, HLA polymorphism has long been recognised as a critical factor in determining allograft acceptance and rejection mechanisms in both renal and bone marrow transplantation (Opelz et al. 1993; Madrigal et al. 1997b), while particular HLA genotypes are associated with a large number of benign and malignant immunologically mediated diseases (Thorsby 1997; Bateman and Howell 1999). Although the MHC also encodes a number of other genes of immunological significance (e. g. the tumour necrosis factor genes), this chapter will focus solely upon the so-called “classical” HLA class I and class II antigens.
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References
Amiel JC (1967) Study of the human leukocyte phenotypes in Hodgkin’s disease. In: Dausset J, Colombani J (eds) Histocompatibility testing. Munksgaard, Copenhagen, pp 79–81
Arguello JR, Little A-M, Pay AL, Gallardo D, Rojas I, Marsh SGE, Goldman JM, Madrigal JA (1998) Mutation detection and typing of polymo rphic loci through double-strand conformation analysis. Nat Genet 18:192–194
Aron Y, Swierczewski E, Lockhart A (1994) A simple and rapid micromethod for genomic DNA extraction from jugal epithelial cells. Allergy 49:788–790
Bateman AC, Howell WM (1999) Human leukocyte antigens and cancer. J Pathol 188:231–236
Bateman AC, Leung ST, Howell WM, Roche WR, Jones DB, Theaker JM (1994) Detection of specimen contamination in routine histopathology by HLA class II typing using the polymerase chain reaction and sequence specific oligonucleotide probing. J Pathol 173:243–248
Bateman AC, Sage DA, Al-Talib RK, Theaker JM, Jones DB, Howell WM (1996) Investigation of specimen mislabelling in paraffin-embedded tissue using a rapid, allele-specific, PCR-based HLA class II typing method. Histopathology 28:169–174
Bateman AC, Hemmatpour SK, Theaker JM, Howell WM (1997) Nested polymerase chain reaction-based HLA class II typing for the unique ident ification of formalin-fixed and paraffinembedded tissue. J Pathol 181:228–234
Bateman AC, Turner SJ, Theaker JM, Howell WM (1998) HLA-DQBl*0303 and *0301alleles influence susceptibility to and prognosis in cutaneous malignant melanoma in the British Caucasian population. Tissue Antigens 52:67–73
Bein G, Haase D, Schult J, Eirmann TH, Kirchner H (1994) Semiautomated HLA-DQBl typing by fluorescent dye photometry of amplified DNA on microtiter plates. Hum Immunol 39:1–8
Bettinotti MP, Mitsuishi Y, Bibee K, Lau M, Terasaki PI (1997) Comprehensive method for the typing of HLA-A, B, and C alleles by direct sequencing of PCR products obtained from genomic DNA. J Immunother 20:425–430
Bidwell JL (1992) Applications of the polymerase chain reaction to HLA class II typing. Vox Sang 63:81–89
Bidwell JL, Bidwell EA, Savage DA, Middleton D, Klouda PT, Bradley BA (1988) A DNA-RFLP typing system that positively identifies serologically well-defined and iII-defined HLA-DRand DQ alleles, including DRw1O. Transplantation 45:640–646
Blasczyk R, Hahn U, Wehling J, Huhn D, Salama A (1995) A complete subtyping of the HLA-A locus by sequence-specific amplification followed by direct sequencing or single-strand conformation polymorphism analysis. Tissue Antigens 46:86–95
Bodmer JG, Marsh SGE, Albert ED, Bodmer WF, Bontrop RE, Dupont B, Erlich HA, Hansen JA, Mach B, Mayr WR, Parham P, Petersdorf EW, Sasazuki T, Schreuder GMTh, Strominger JL, Svejgaard A, Terasaki PI (1999) Nomenclature for factors of the HLA system, 1998. Eur J Immunogenet 26:81–116
Bodmer WF (1995) Evolution and function of the HLA region. Cancer Surv 22:5–16
Braud VM, Allan DSJ, McMichael AJ (1999) Functions of nonclassical MHC and non-MHCencoded class I molecules. Curr Opin Immunol 11:100–108
Brodsky FM, Lem L, Bresnahan PA (1996) Antigen processing and presentation. Tissue Antigens 47:464–471
Brown JH, Jardetsky TS, Gorga JC, Stern LJ, Urban RG, Strominger JL, Wiley DC (1993) Threedimensional structure of the human class II histocompatibility antigen HLA-DRI. Nature 364:33–39
Browning MJ, Krausa P, Bodmer JG (1994) Low resolution HLA-A locus typing by multiplex ARMS-PCR. Hum Immunol 39:134 (abstract)
Bunce M, O’Neill CM, Barnardo MCNM, Krausa P, Browning MJ, Morris PJ, Welsh KI (1995) Phototyping: comprehensive DNA typing for HLA-A, B, C, DRB1, DRB3, DRB4, DRB5 and DQBl by PCR with 144 primer mixes utilizing sequence-specific primers (PCR-SSP). Tissue Antigens 46:355–367
Clay TM, Bidwell JL, Howard MR, Bradley BA (1991) PCR fingerprinting for selection of HLA matched unrelated marrow donors. Lancet 337:1049–1052
Day IN, Spanakis E, Palamand D, Weavind GP, O’Dell SD (1998) Microplate-array diagonal-gel electrophoresis and melt-MADGE: tool s for molecular-genetic epidemiology. Trends Biotechnol 16:287–290
Day IN, O’Dell SD, Spanakis E, Weavind GP (1999) Microplate array diagonal gel electrophoresis (MADGE), CpG-PCR and temporal thermal ramp-MADGE (Melt-MADGE) for single nucleotide analyses in populations. Genet Anal 14:197–204
Dyer PA, Claas FHJ (1997) A future for HLA matching in clinical transplantation. Eur J Immunogenet 24:17–28
Faas SJ, Menon R, Braun ER, Rudert WA, Trucco M (1996) Sequence-specific priming and exonuclease-released fluorescence detection of HLA-DQBl alleles. Tissue Antigens 48:97112
Ferencik S, Grosse-Wilde H (1993) A simple photometric detection method for HLA-DRBI specific PCR-SSPproducts. Eur J Immunogenet 20:123–125
Fernandez-Vina MA, Bignon JD (1997) Primers and oligonucleotide probes (SSOP) used for DNA typing of HLA class II alleles. In: Charron D (ed) Genetic diversity of HLA — functional and medical implications. Proceedings of the 12th International Histocompatibility Workshop and Conference, vol 1. EDK, Paris, pp 584–595
Giorda R, Lampasona V, Kocova M, Trucco M (1993) Non-radioisotopic typing of human leukocyte antigen class II genes on microplates. Biotechniques 15:918–925
Guttridge MG, Thompson J, Worwood M, Darke C (1998) Rapid detection of genetic mutations associated with haemochromatosis. VoxSang 75:253–256
Hemmatpour SK, Dunn PPJ, Evans PR, Green A, Howell WM (1998) Functional characterization and exon 2-intron 2-exon 3 gene sequence of HLA-B*2712 as found in a British family. Eur J Immunogenet 25:395–402
Howell WM, Holgate ST (1996) Human leukocyte antigen genes and allergic disease. In: Hall IP (ed) Genetics of asthma and atopy. Karger, Basel, pp 53–70
Howell WM, Navarrete C (1996) The HLA system: an update and relevance to patient-donor matching strategies in clinical transplantation. Vox Sang 71:6–12
Howell WM, Evans PR, Wilson PJ, Spellerberg MB, Smith JL (1989) Comparison of serological, cellular and DNA-RFLP HLA matching in the selection of related bone marrow donors. Bone Marrow Transplant 4:63–68
Howell WM, Leung ST, Jones DB, Nakshabendi I, Hall MA, Lanchbury JS, Ciclitira PJ, Wright DH (1995) HLA-DRB,-DQA and-DQB polymorphism in celiac disease and enteropathyassociated T-cell lymphoma: common features and additional risk factors for malignancy. Hum Immunol 43:29–37
Kennedy LJ, Poulton KV, Thomson W, Williams F, Middleton D, Howell WM, Tarassi K, Papasteriades C, Albert E, Fleischhauer K, Chandanayingyong D, Tiercy JM, Iuji T, Tokunagaai JK, Ollier WER (1997) HLAclass I DNA typing using sequence specific oligonucleotide probes. In: Charron D (ed) Genetic diversity of HLA — functional and medical implications. Proceedings of the 12th International Histocompatibility Workshop and Conference, vol 1. EDK, Paris, pp 216–225
Kimura A, Sasazuki T (1992) 11th international histocompatibility wokshop reference protocol for the HLA DNA-typing technique. In: Tsuji K, Aizawa M, Sasazuki T (eds) HLA 1991. Proceedings of the 11 th International Histocompatibility Workshop, vol 1. Oxford University Press, Oxford, pp 397–419
Kimura A, Fukuda Y, Hori H, Hoshino S, Sasazuki T, Dohi K (1993) Polymerase chain reactionsinglestrand conformation polymorphism analysis of HLA-DP genes and its application in transplantation. Transplant Proc 25:199–202
Krausa P, Browning M (1996) Detection of HLA gene polymorphism. In: Browning M, McMichael A (eds) HLA and MHC: genes, molecules and function. BIOS Scientific Publishers Limited, Oxford, pp 113–137
Lalvani A, Hill AVS (1998) Cytotoxic T-lymphocytes against malaria and tuberculosis: from natural immunity to vaccine design. Clin Sci 95:531–538
Lanier LL (1998) Follow the leader: NK cell receptors for classical and non-classical MHC class 1. Cell 92:705–707
Lienert K, Russ G, Lester S, Bennett G, Gao X, McCluskey J (1996) Stable inheritance of an HLA “blank” phenotype associated with a structural mutation in the HLA-A*0301 gene. Tissue Antigens 48:187–191
Luedeck H, Biasczyk R (1997) Fluorotyping of HLA-C: differential detection of amplicons by sequence-specific priming and fluorogenic probing. Tissue Antigens 50:627–638
Madrigal JA, Arguello R, Scott I, Avakian H (1997a) Molecular histocompatibility typing in unrelated donor bone marrow transplantation. Blood Rev 11:105–117
Madrigal JA, Scott I, Arguello R, Szydlo R, Little A-M, Goldman JM (1997b) Factors influencing the outcome of bone marrow transplants using unrelated donors. Immunol Rev 15:153–166
Metcalfe P, Waters AH (1993) HPA-1 typing by PCR amplification with sequence-specific primers (PCR-SSP): a rapid and simple technique. Br J Haematol 85:227–229
Miller SA, Dykes DD, Polesky HF (1988) A simple salting out procedure for extracting DNAfrom human nucleated cells. Nucleic Acids Res 16:12–15
Mittal KK, Mickey MR, Singal DP, Terasaki PI (1968) Serotyping for homotransplantation: refinement of micro droplet lymphocyte cytotoxicity test. Transplantation 6:913–927
Mytilineos J, Scherer S, Opelz G (1990) Comparison of RFLP-DR beta and serological HLA-DR typing in 1500 individuals. Transplantation 50:870–873
Newton CR, Graham A, Heptinstall LE, Powell SJ, Summers C, Kalsheker N, Smith JC, Markham AF (1989) Analysis of any point mutation in HLA. The amplification refractory mutation system (ARMS). Nucleic Acids Res 17:2503–2516
Olerup O, Zetterquist H (1992) HLA-DR typing by PCR amplification with sequence-specific primers (PCR-SSP) in 2 hours: an alternative to serological DR typing in clinical practice including donor-recipient matching in cadaveric transplantation. Tissue Antigens 39:225–235
Opelz G, Mytilineos J, Scherer S, Dunckley H, Trejaut J, Chapman J, Fischer G, Fae I, Middleton D, Savage D, Bignon J-D, Bensa J-C, Noreen H, Albert E, Albrecht G, Schwarz V (1993) Analysis of HLA-DR matching in DNA-typed cadaver kidney transplants. Transplantation 55: 782–785
Parham P (1992) Typing for HLA class I polymorphism: past, present and future. Eur J Immunogenet 19:347–359
Perrey C, Turner SJ, Pravica V, Howell WM, Hutchinson IV (1999) ARMS-PCR methodologies to determine IL-10, TNF-a, TNF-β and TGF-β1 polymorphisms. Transplant Immunol 7:127–128
Powis SH, Teisserenc H (1997) TAP, LMP and HLA-DM typing protocols. In: Charron D (ed) Genet ic diversity of HLA — functional and medical implications. Proceedings of the 12th International Histocompatibility Workshop and Conference, vol I. EDK, Paris, pp 226–235
Procter J, Crawford J, Bunce M, Welsh KI (1997) A rapid molecular method (polymerase chain reaction with sequence-specific primers) to genotype for ABOblood group and secretor status and its potential for organ transplants. Tissue Antigens 50:475–483
Ramon DS, Arguello JR, Cox ST, McWhinnie A, Little A-M, Marsh SGE, Madrigal JA (1998) Application of RSCA for the typing of HLA-DPB1. Hum Immunol 59:734–747
Savage DA, Tang JP, Wood NA, Evans J, Bidwell JL, Wee JL, Oei AA, Hui KM (1996) A rapid HLADRBI* 04 subtyping method using PCR and heteroduplex generators. Tissue Antigens 47: 284–292
Shintaku S, Fukuda Y, Kimura A, Hoshino S, Tashiro H, Sasazuki T, Dohi K (1993) DNA conformation polymorphism analysis of DR52 associated HLA-DR antigens by polymerase chain reaction: a simple, economical and rapid examination for HLA matching in transplantation. Ipn J Med Sci Biol 46:165–181
Singal DP, Ye M (1998) HLA-DM polymorphisms in patients with rheumatoid arthritis. J Rheumato 125: 1295–1298
Spielman RS, McGinnis RE, Ewens WJ (1993) Transmission test for linkage disequilibrium: the insulin gene region and insulin dependent diabetes mellitus (IDDM). Am J Hum Genet 52: 506–516
Stern LJ, Wiley DC (1994) Antigenic peptide binding by class I and class II histocompatibility proteins. Structure 2:245–251
Tatari Z, Fortier C, Bobrynina V, Loiseau P, Charron D, Raffoux C (1995) HLA-Cwanalysis by PCRrestriction fragment length polymorphism: study of known and additional alleles. Proc Natl Acad Sci USA 92:8803–8807
Thorsby E (1997) Invited anniversary review: HLA and disease. Hum Immunol 53:1–11
Thorsby E, Ronningen KS (1992) Role of HLA genes in predisposition to develop insulindependent diabetes mellitus. Ann Med 24:523–531
Trowsdale J (1995) “Both man & bird & beast”: comparative organization of MHC genes. Immunogenetics 41:1–17
Tuokko J, Pushnova E, Yli-Kerttula U, Toivanen A, Ilonen J (1998) TAP2 alleles in inflammatory arthritis. Scand J Rheumatol 27:225–229
Uryu N, Maeda M, Ota M, Tsuji K, Inoko H (1990) A simple and rapid method for HLA-DRBand-DQB typing by digestion of PCR-amplified DNA with allele specific restriction endonucleases. Tissue Antigens 35:20–31
Van Ham SM, Tijn EPM, Lillemeir BF, Gruneberg U, van Meijgaarden KE, Pastoors L, Verwoerd D, Tulp A, Canas B, Rahman D, Ottenhof THM, Pappin DJC, Trowsdale J, Neefjes J (1997) HLADO is a negat ive modulator of HLA-DM-mediated MHC class II peptide loading. Curr Biol 7:950–957
Vogt AB, Kropshofer H, Hammerling GJ (1997) How HLA-DM affects the peptide repertoire bound to HLA-DR molecules. Hum Immunol 54:170–179
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Howell, W.M., Poole, K.L. (2002). The Special Case of HLA Genes: Detection and Resolution of Multiple Polymorphic Sites in a Single Gene. In: Day, I.N.M. (eds) Molecular Genetic Epidemiology — A Laboratory Perspective. Principles and Practice. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-56207-5_6
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DOI: https://doi.org/10.1007/978-3-642-56207-5_6
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