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Fluorescence Spectroscopy, Imaging and Probes pp 241–252Cite as

Assessment of Membrane Fluidity in Individual Yeast Cells by Laurdan Generalised Polarisation and Multi-photon Scanning Fluorescence Microscopy

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Part of the book series: Springer Series on Fluorescence ((SS FLUOR,volume 2))

Abstract

Here we describe techniques that we developed for monitoring membrane fluidity of individual yeast cells during environmental adaptation and physiological changes. Multi-photon scanning fluorescence microscopy using laurdan as a membrane probe enables determination whether fluidity changes seen by spectroscopy reflect universal responses or changes only of subpopulations.

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Authors
  1. R. P. Learmonth
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  2. E. Gratton
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Editor information

Editors and Affiliations

  1. Institute of Molecular Biological Sciences, Amsterdam, Vrije Universiteit, De Boelelaan 1087, 1081 HV, Amsterdam, The Netherlands

    Ruud Kraayenhof

  2. MicroSpectroscopy Center, Wageningen University, Dreijenlaan 3, 6703 HA, Wageningen, The Netherlands

    Antonie J. W. G. Visser

  3. Dept. of Molecular Biophysics, University of Utrecht, Princetonplein 1, 3584 CC, Utrecht, The Netherlands

    Hans C. Gerritsen

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© 2002 Springer-Verlag Berlin Heidelberg

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Learmonth, R.P., Gratton, E. (2002). Assessment of Membrane Fluidity in Individual Yeast Cells by Laurdan Generalised Polarisation and Multi-photon Scanning Fluorescence Microscopy. In: Kraayenhof, R., Visser, A.J.W.G., Gerritsen, H.C. (eds) Fluorescence Spectroscopy, Imaging and Probes. Springer Series on Fluorescence, vol 2. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-56067-5_14

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  • DOI: https://doi.org/10.1007/978-3-642-56067-5_14

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-62732-3

  • Online ISBN: 978-3-642-56067-5

  • eBook Packages: Springer Book Archive

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