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Arenaviruses II pp 239–261Cite as

Junin Virus Vaccines

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Part of the book series: Current Topics in Microbiology and Immunology ((CT MICROBIOLOGY,volume 263))

Abstract

Argentine hemorrhagic fever (AHF) was recognized as a new clinical entity from the richest farming region of Argentina in the 1950s (Arribalzaga 1955). The etiologic agent of this disease, Junin virus (JUN), was isolated in 1958 (Parodi et al. 1958; Piroski et al. 1959). JUN belongs to the Arenaviridae family, which includes other rodent-borne pathogens which are important causes of hemorrhagic fever in Africa, (Lassa) and South America (Machupo, Guanarito, and Sabia viruses). The Arenaviridae comprises at least 20 recognized members. Arenaviruses are enveloped RNA viruses, and are divided into two groups, with low level antigenic relatedness: the Old World group, and New World group or Tacaribe complex. Two antigenic subgroups were defined within the New World arenaviruses. JUN is contained into the first group, together with Amapari, Latino, Machupo and Tacaribe viruses. Phylogenetic analysis have shown that Old World and New World arenaviruses occupied two distinct clades, and that New World arenaviruses comprise three evolutionary lineages, named A, B, and C. JUN is contained in lineage B, together with the other three agents causing South American hemorrhagic fevers (Enria et al. 1999a).

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References

  • Agnese G (1999) Histaria de una enfermedad, imaginario y espacio rural: La fiebre hemorrágica argentina — 1943–1963. Tesis para optar al grado de Licenciatura en Histaria. 5.2. Experiencias en el Hospital Neuropsiquiátrico. Pontificia Universidad Catolica Argentina. Facultad de derecho y Ciencias Sociales. Instituto de Historia. Rosario. Argentina.

    Google Scholar 

  • Albariño CJ, Ghiringhelli PD, Posik DM, et al. (1997) Molecular characterization of attenuated Junin virus strains. J Gen Virol 78: 1605–1610

    PubMed  Google Scholar 

  • Alibek K (1999) Biohazard. Biodefense (Junin virus: pp. 42, 113, 126, 202, 281; Machupo virus: pp. 18, 42, 113, 118, 126, 202, 234, 281). Random House, New York, NY

    Google Scholar 

  • Arribalzaga RA (1955) Una nueva enfermedad epidémica a germen desconocido: hipertermia nefrotóxica, leucopénica y enantemética. Día Médico 27:1204–1210

    PubMed  CAS  Google Scholar 

  • Auperin DD, Esposito JJ, Lange JV, et al. (1988) Construction of a recombinant vaccinia virus expressing the Lassa virus glycoprotein gene and protection of guinea pigs from lethal Lassa virus infection. Virus Res 9:233–248

    Article  PubMed  CAS  Google Scholar 

  • Avila MM, Samoilovich SR, Weissenbacher MC (1979) Infección del cobayo con la cepa atenuada del virus Junin XJCI3. Medicina (B. Aires) 39:597–603

    CAS  Google Scholar 

  • Avila MM, Laguens RM, Laguens RP, et al. (1981) Selectividad tisular e indicadores de virulencia de tres cepas del virus Junin. Medicina (B. Aires) 41:157–166

    CAS  Google Scholar 

  • Avila MM, Frigerio MJ, Weber EL, et al. (1985) Attenuated Junin virus infection in Callithrix jacchus. J Med Virol 15:93–100

    Article  PubMed  CAS  Google Scholar 

  • Barrera Oro JG, Girola RA, Frugone G (1967) Estudios inmunológicos con virus Junin: II. inmunidad adquirida por cobayos inoculados con virus inactivado por formol. Medicina (B. Aires) 27:279–282

    Google Scholar 

  • Barrera Oro JG and Eddy GA (1982) Characteristics of Candidate live attenuated Junin Virus Vaccine. IV International Conference on Comparative Virology, Banff, Alberta, Canada. Abstract S4–10

    Google Scholar 

  • Barrera Oro JG, McKee KT Jr, Kuehne AI, et al. (1985) Preclinical trials of a live, attenuated Junin virus vaccine in rhesus macaques. Institut Pasteur Symposium on Vaccines and Vaccinations. Paris, France. Abstract P28

    Google Scholar 

  • Barrera Oro J, MacDonald C, Kenyon R, et al. (1987) Virus isolation and immune response in humans inoculated with a live-attenuated Junin virus (JV) vaccine. VIIth International Congress of Virology. Edmonton, Alberta, Canada. Abstract R3.45

    Google Scholar 

  • Barrera Oro JG, Lupton HW, Jahrling PB, et al. (1988) Cross protection against Machupo virus with Candid #1 live-attenuated Junin virus vaccine. I. The post-vaccination prechallenge immune response. Second International Conference on Comparative Virology, Mar del Plata, Argentina. Abstract P.E.1

    Google Scholar 

  • Barrera Oro JG, McKee KT Jr (1992) Hacia una vacuna contra la Fiebre Hemorrágica Argentina. Bol of Sanit Panam 112:296–305

    Google Scholar 

  • Boxaca MC, Guerrero LB de, Weber L, Malumbres E (1981) Protección inducida en cobayo por la variente XJ0 del virus Junin. Medicina (B. Aires) 41:25–34

    CAS  Google Scholar 

  • Boxaca MC, Guerrero LB, de Malumbres E (1984) Modification of Junin virus neurotropism in the guinea pig model. Acta Virol 28:198–203

    PubMed  CAS  Google Scholar 

  • Carballal G, Cossio PM, de la Vega MI, et al. (1982) Neurovirulencia de la cepa XJCI3, de virus Junin en un primate sudamericano. Tercer Congreso de Microbiología, Córdoba, Argentina, Compendio 186

    Google Scholar 

  • Carballal G, Videla C, Oubiña JR, et al. (1985) Antigenos inactivados de virus Junin. Medicina (B. Aires) 45: 153–158

    CAS  Google Scholar 

  • Casals J (1965) Serological studies on Junin and Tacaribe viruses. Am J Trop Med Hyg 14:794–795

    PubMed  CAS  Google Scholar 

  • Clegg JC, Lloyd G (1987) Vaccinia recombinant expressing Lassa-virus internal nucleocapsid protein protects guinea pigs against Lassa fever. Lancet 2(8552):186–188

    Article  PubMed  CAS  Google Scholar 

  • Contigiani MS, Sabattini MS (1977) Virulencia diferencial de cepas de virus Junin por marcadores biológicos en ratones y cobayos. Medicina (B. Aires) 37:244–251

    Google Scholar 

  • Coto CE, Damonte EB, Calello MA, et al. (1980) Protection of guinea pigs inoculated with Tacaribe virus against lethal doses of Junin virus. J Infect Dis 141:389–393

    Article  PubMed  CAS  Google Scholar 

  • Cresta B, Padula P, de Martinez Segovia ZM (1980) Biological properties of Junin virus proteins: I, identification of the immunogenic glycoprotein. Intervirology 13:284–288

    Article  PubMed  CAS  Google Scholar 

  • D’Aiutolo AC, Lampuri JS, Coto CE (1979) Reactivación del virus Junin inactivado por la luz ultravioleta. Medieina (B. Aires) 39:801

    Google Scholar 

  • Damonte EB, Calello MA, Coto CE, et al. (1981) Inmunización de cobayos contra la fiebre hemorrágica argentina con virus Tacaribe replicado en células diploides humanas. Medicina (B. Aires) 41:467–470

    CAS  Google Scholar 

  • Damonte EB, Mersich SE, Candurra NA, et al. (1986) Cross reactivity between Junin and Tacaribe viruses as determined by neutralization test and immunoprecipitation. Med Microbiol Immunol 175:85–88

    Article  PubMed  CAS  Google Scholar 

  • Downs WG, Anderson CR, Aitken THG, et al. (1963) Tacaribe virus, a new agent isolated from Artibeus bats and mosquitoes in Trinidad. Am J Trop Med Hyg 12:639–646

    Google Scholar 

  • Eddy GA, and Barrera Oro JG (1977) Discusión general. Medicina (B. Aires) 37(Supl 3):257–259

    Google Scholar 

  • Enria DA, Briggiler AM, Fernández NJ, et al. (1984) Importance of dose of neutralizing antibodies in treatment of Argentine hemorrhagic fever with immune plasma. Lancet 8397:255–256

    Article  Google Scholar 

  • Enria DA, Damilano AJ, Briggiler AM, et al. (1985) Síndrome neurológico tardío en enfermos de Fiebre Hemorrágica Argentina tratados con plasma inmune. Medicina (B. Aires) 45(6):615–620

    CAS  Google Scholar 

  • Enria DA, Maiztegui JI (1994) Antiviral treatment of Argentine hemorrhagic fever. Antivir Res 23:23–31

    Article  PubMed  CAS  Google Scholar 

  • Enria DA, Briggiler AM, Feuillade MR (1998) An overview of the epidemiological, ecological and preventive hallmarks of Argentine Hemorrhagic Fever (Junin virus). Bull Inst Pasteur 96: 103–114

    Article  Google Scholar 

  • Enria D, Bowen M, Mills JN, et al. (1999a) I Arenaviruses. In: Guerrant RL, Walker DH, Weller PF, Saunders WB (eds) Tropical Infectious Diseases: Principles, Pathogen & Practice. New York, pp 1189–1212

    Google Scholar 

  • Enria DA, Feuillade MR, Levis SC, et al. (1999b) Impact of vaccination of a high risk population for Argentine hemorrhagic fever with a live attenuated Junin virus vaccine. In: Saluzzo JF., Dodet B (eds) Factors in the Emergence and Control of Rodent-borne Viral Diseases. Elsevier SAS, Paris, France, pp 273–280

    Google Scholar 

  • Farrington CP (1993) Estimation of vaccine effectiveness using the screening method. Int J Epidemiol 22:742–746

    Article  PubMed  CAS  Google Scholar 

  • Feuillade MR, Sabattini M, Enria D (1997) Vacunación de una poblacion expuesta a riesgo natural de adquirir la fiebre hemorragica Argentina. Evolucion de la incidencia. V Congreso Latinoamericano de Medicina Tropical. 3–7 March 1997, Havana, Cuba, Abstracts:650

    Google Scholar 

  • Fisher-Hoch SP, McCormick JB, Auperin DD, et al. (1989) Protection of rhesus monkeys from fatal Lassa fever by vaccination with a recombinant vaccinia virus containing the Lassa virus glycoprotein gene. Proc Nat Acad Sci USA 86:317–321

    Article  PubMed  CAS  Google Scholar 

  • García J, Calderón G, Sabattini M, et al. (1996) Infección por virus Junin (VJ) de Calomys musculinus (Cm) en áreas con diferente situación epidemiológica para la Fiebre Hemorrágica Argentina (FHA). Medicina (B. Aires) 56:624

    Google Scholar 

  • Ghiringhelli PD, Albarino CG, Piboul I, Romanowski V (1997) The glycoprotein precursor gene of the attenuated Junin Virus vaccine strain (Candid #1). Am J Trop Med Hyg 56(2):216–225

    PubMed  CAS  Google Scholar 

  • Guerrero LB, de, Weissenbacher MC, Parodi AS (1969) Inmunización contra la fiebre hemorrágica argentina con una cepa atenuada del virus Junin; I: estudio de una cepa modificada del virus Junin, inmunización de cobayos. Medicina (B. Aires) 29: 1–5

    Google Scholar 

  • Guerrero LB (1977) Vacunas experimentales contra la fiebre hemorragica Argentina. Medicina (B. Aires) 37:252–259

    Google Scholar 

  • Guerrero LB de, Boxaca MC (1980) Estudio preliminar de una variante atenuada del virus Junin derivada de la cepa prototipo XJ. Medicina (B. Aires) 40:267–274

    Google Scholar 

  • Guerrero LB de, Boxaca MC, Rabinovich RD, et al. (1983) Evolución de la infección en cobayos infectados con la variante atenuada XJ0 del virus Junin. Rev Argent Microbiol 15:205–212

    PubMed  Google Scholar 

  • Guerrero LB de, Boxaca MC, Malumbres E, et al. (1985) Pathogenesis of attenuated Junin virus in the guinea pig model. J Med Virol 15: 197–202

    Article  PubMed  Google Scholar 

  • Jahrling PB, Trotter RW, Barrera Oro JG, et al. (1988) Cross-protection against Machupo virus with Candid #1 Junin virus vaccine: III. post-challenge clinical findings. Second International Conference on the Impact of Viral Diseases on the Development of Latin American Countries and the Caribbean Region, Mar del Plata, Argentina, abstract PE3

    Google Scholar 

  • Kenyon RH, McKee K, Barrera Oro JG, et al. (1989) Protective efficacies in rhesus monkeys of Candid 1 strain Junin virus (JV) and Tacaribe virus against aerosol challenge with virulent JV: 38th Annual Meeting of the American Society of Tropical Medicine and Hygiene, Honolulu. Abstract 136

    Google Scholar 

  • Kurstak E (1994) Modern vaccinology: Progress towards the global control of Infectious Diseases. In:Kurstak E (ed) Modern Vaccinology, Plenun Medical Book Company, New York, London, pp 1–9

    Google Scholar 

  • Laguens RM, Avila MM, Samoilovich SR, et al. (1983) Pathogenicity of an attenuated strain (XJCI3) of Junin virus: morphological and virological studies in experimentally infected guinea pigs. Intervirology20: 195–201

    Article  PubMed  CAS  Google Scholar 

  • López N, Scolaro L, Rossi C, et al. (2000) Homologous and heterologous glycoproteins induce protection against Junin virus challenge in guinea pigs. J Gen Vir: 1273–1281

    Google Scholar 

  • Lupton HW, Jahrling PB, Barrera Oro JG, et al. (1988) Cross-protection against Machupo virus with Candid #1 live-attenuated Junin virus vaccine: II, post-challenge virological and immunological findings. Second International Conference on the Impact of Viral Diseases on the Development of Latin American Countries and the Caribbean Region, Mar del Plata, Argentina, abstract PE2

    Google Scholar 

  • MacDonald C, McKee K, Peters C, et al. (1987) Initial clinical assessment of humans inoculated with a live-attenuated Junin virus vaccine. In: Programs and abstracts of the VII International Congress of Virology (Edmonton, Canada). Abstract R3.27

    Google Scholar 

  • MacDonald C, McKee K, Meegan J, et al. (1988) Initial evaluation in humans of a live-attenuated vaccine against Argentine hemorrhagic fever. In: Proceedings of the XVI Army Science Conference (Hampton, Virginia). US Army

    Google Scholar 

  • McKee KT, Jr, Barrera Oro JG, Kuchne AI, et al. (1992) Candid No 1 Argentine Hemorrhagic Fever vaccine Protects Against Lethal Junin Virus Challenge in Rhesus Macaques. Intervirology 34:154–163

    Article  PubMed  Google Scholar 

  • McKee KT Jr, Green DE, Mahlandt BG, et al. (1985) Infection of Cebus monkeys with Junin virus.Medicina (B. Aires) 45:144–152

    Google Scholar 

  • Maiztegui JI, and Sabattini MS (1977) Extensión progresiva del área endémica de Fiebre hemorrrágica argentina. Medicina (B. Aires) 37:162–166

    Google Scholar 

  • Maiztegui JI, Fernández NJ, Damilano AJ, (1979) Efficacy of immune plasma in treatment of Argentine hemorrhagic fever and association between treatment and late neurological syndrome. Lancet8154: 1216–1217

    Article  Google Scholar 

  • Maiztegui JI, Feuillade MR, Briggiler AM (1986) Progressive extension of the endemic area and changing incidence of Argentine hemorrhagic fever. Med Microbiol Inmunol 175: 149–152

    Article  CAS  Google Scholar 

  • Maiztegui J, Levis S, Enria D, et al. (1988) Inocuidad e inmunogenicidad en seres humanos de la cepa Candid 1 de virus Junin. Medicina (B. Aires) 6:660

    Google Scholar 

  • Maiztegui JI, McKee KT Jr (1989) Inoculation of human volunteers with a vaccine against Argentine hemorrhagic fever. In: Program and abstracts of the VI International Conference on Comparative and Applied Virology (Banff. Canada). Abstract S4

    Google Scholar 

  • Maiztegui JI, McKee KT Jr, Barrera Oro JG, et al. (1998) Protective efficacy of a live attenuated vaccine against Argentine Hemorrhagic Fever. J Infect Dis 177:277–283

    Article  PubMed  CAS  Google Scholar 

  • Malumbres E, Boxaca MC, Guerrero LB de, et al. (1984) Persistence of attenuated Junin virus strains in guinea pigs infected by IM or IC routes. J Infect Dis 149:1022

    Article  PubMed  CAS  Google Scholar 

  • Martinez Segovia ZM de, Arguelles G, Tokman A (1980) Capacidad antigénica del virus Junin inactivado mediante oxidación fotodinámica. Medicina (B. Aires) 40:156–160

    Google Scholar 

  • Mettler NE, Casals J, Shope RE (1963) Study of the antigenic relationships between Junin virus. the etiological agent of Argentinean hemorrhagic fever, and other arthropod-borne viruses. Am J Trop Med Hyg 12:647–652

    Google Scholar 

  • Mettler N (1969) Argentine hemorrhagic fever: current knowledge. PAHO Scientific Publication 183, Washington DC, Pan American Health Organization.

    Google Scholar 

  • Mills IN, Ellis BA, McKee KT, et al. (1992) A longitudinal study of Junin virus activity in the rodent reservoir of Argentine hemorrhagic fever. Am J Trop Med Hyg 47:749–763

    PubMed  CAS  Google Scholar 

  • Morrison HG, Bauer SP, Lange JV, et al. (1989) Protection of guinea pigs from Lassa fever byvaccinia virus recombinants expressing the nucleoprotein or the envelope glycoproteins of Lassa virus. Virology 171:179–188

    Article  PubMed  CAS  Google Scholar 

  • Parodi AS, Geenway DJ, Rugiero HR, et al. (1958) Sobre la etiología del brote epidemico de Junin. Día Médico 30:2300–2301

    PubMed  CAS  Google Scholar 

  • Parodi AS, Coto CE (1964) Inmunización de cobayos contra el virus Junin por inoculación del virus Tacaribe. Medicina (B. Aires) 24:151–153

    CAS  Google Scholar 

  • Parodi AS, de Guerrero LB, Weissenbacher M (1965) Fiebre hemorrágica argentina: vacunación con virus Junin inactivado. Ciencia e Investigación 21:132–133

    Google Scholar 

  • Pirosky I, Martini P, Zuccarini J, et al. (1959) Virosis hemorrágica del noroeste bonaerense (endemoepidímica, febril, exantemática y leucopínica): V. la vacuna específica y la vacunación. Orientación Médica 8:743–744

    Google Scholar 

  • Rico-Hesse R (1999) Vaccines for emergent American arenaviruses. In: Saluzzo JB, Dodet B (eds) Factors in the emergence and control of rodent-borne viral diseases. Editions scientifiques et medicales Elsevier SAS, pp 267–272

    Google Scholar 

  • Rugiero HA, Astarloa L, Gonzalez Cambaceres C, et al. (1969) Inmunización contra la fiebre hemorrágica argentina con una cepa atenuada de virus Junin: II, inmunización de voluntarios, análisis clínico y del laboratorio. Medicina (B. Aires) 29:81–92

    CAS  Google Scholar 

  • Rugiero HA, Magnoni C, Cintora FA, et al. (1974) Inmunización contra la fiebre hemorrágica argentina con una cepa atenuada de virus Junin: análisis de 636 vacunados. Pren Med Argent 61:231–240

    Google Scholar 

  • Rugiero HA, Magnoni C, Guerrero LB de, et al. (1981) Persistence of antibodies and clinical evaluation in volunteers 7 to 9 years following the vaccination against Argentine hemorrhagic fever. J Med Virol 7:227–232

    Article  Google Scholar 

  • Sabattini MS and Contigiani MS (1982) Ecological and biological factors influencing the maintenance of arenaviruses in nature, with special reference to the agent of Argentine hemorrhagic fever. In: Pinheiro FD (ed) International Symposium on Tropical Arbovirus and Hemorrhagic Fevers, Academia Brasilera de Ciencias, Rio de Janeiro, pp 251–262

    Google Scholar 

  • Samoilovich SR, Pecci Saavedra J, Frigerio MJ, et al. (1984) Nasal and intra thalamic inoculations of primates with Tacaribe virus: protection against Argentine hemorrhagic fever and absence of neurovirulence. Acta Virol 28:277–281

    PubMed  CAS  Google Scholar 

  • Samoilovich SR, Calello MA, Laguens RP, et al. (1988) Long-term protection against Argentine hemorrhagic fever in Tacaribe virus infected marmosets: virologic and histopathologic findings. J Med Virol 24:229–236

    Article  PubMed  CAS  Google Scholar 

  • Tauraso N, Shelokov A (1965) Protection against Junin virus by immunization with live Tacaribe virus. Proc Soc Exp Biol Med 119:608–611

    PubMed  CAS  Google Scholar 

  • US Food and Drug Administration’s Invcstigational New Drug Application #2257

    Google Scholar 

  • Videla C, Carballal G, Remorini P, et al. (1989) Formalin inactivated Junin virus: immunogenicity and protection assays. J Med Virol 129:215–220

    Article  Google Scholar 

  • Wallen KH (1963) Demonstration of inapparent heterogeneity in a population of an animal virus by single-burst analyses. Virology 20:230–234

    Article  Google Scholar 

  • Weissenbacher M, Guerrero LB, de, Help G, et al. (1969) Inmunización contra la fiebre hemorrágica argentina con una cepa atenuada de virus Junin: III, reacciones serológicas en voluntarios. Medicina (B. Aires) 29:88–92

    CAS  Google Scholar 

  • Weissenbacher MC, Coto CE, Calello MA (1975) Cross-protection between Tacaribe complex viruses: presence of neutralizing antibodies against Junin virus (Argentine hemorrhagic fever) in guinea pigs infected with Tacaribe virus. Intervirology 6:42–49

    Article  PubMed  Google Scholar 

  • Weissenbacher MC, Coto CE, Calello MA, et al. (1982) Cross-protection in non-human primates against Argentine hemorrhagic fever. Infect Inmun 35:425–430

    CAS  Google Scholar 

  • Weissenbaeher MC, Damonte EB (1983) Fiebre hemorrágica argentina. Ade Microbiol Enf Infect 2: 119–171

    Google Scholar 

  • Weissenbacher MC, Laguens RP, Coto CE (1987) Argentine hemorrhagic fever. Curr Top Microbiol Immunol 134:79–116

    Article  PubMed  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Instituto Nacional de Enfermedades Virales Humanas (INEVH) “Dr. Julio I. Maiztegui”, Monteagudo 2510, 2700, Pergamino, Argentina

    D. A. Enria

  2. Instituto Malbran, CONICET, Argentina

    J. G. Barrera Oro

  3. The Salk Institute, USA

    J. G. Barrera Oro

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  1. D. A. Enria
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  1. Division of Virology Department of Neuropharmacology, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA, 92037, USA

    Michael B. A. Oldstone

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Enria, D.A., Oro, J.G.B. (2002). Junin Virus Vaccines. In: Oldstone, M.B.A. (eds) Arenaviruses II. Current Topics in Microbiology and Immunology, vol 263. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-56055-2_12

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