Abstract
Often the aim of a transformation is to obtain at least one transformant that can be used in further experiments. This transformant should contain either a linear fragment integrated at the correct position in the genome or a plasmid. On the other hand, for transformation with a plasmid library, for example, high transformation efficiency is required. Therefore, the proce-dures for transformation of K. lactis described here are optimized to obtain as many transformants as possible.
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Kooistra, R.A., Steensma, H.Y. (2003). Transformation of Kluyveromyces lactis . In: Wolf, K., Breunig, K., Barth, G. (eds) Non-Conventional Yeasts in Genetics, Biochemistry and Biotechnology. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-55758-3_26
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DOI: https://doi.org/10.1007/978-3-642-55758-3_26
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