Reaching the Macrophage: Routes of Delivery
The functional heterogeneity of macrophages based on anatomical localization and receptor expression as well as their versatile way through which they react to different stimuli makes them key players in many processes of the body. To stimulate or downregulate their functions in therapeutic ways using their extremely efficient phagocytosis capacity, one has to consider that they are often located at sites that are hard to reach. Local administration of drugs is therefore often the method of choice whereby liposomes seem to be an ideal vehicle through their versatility and relative ease of handling, as well as their efficient uptake by macrophages. Especially the use of the macrophage suicide technique has enabled the study of many functions of the macrophage. An overview is given of the many routes that can be used to reach the macrophage at different sites and the effects this has on the function of the cell.
KeywordsLiposomes Phagocytosis Clodronate
Unable to display preview. Download preview PDF.
- Biewenga J, van der Ende MB, Krist LF, Borst A, Ghufron M, van Rooijen N (1995) Macrophage depletion in the rat after intraperitoneal administration of liposome-encap-sulated clodronate: depletion kinetics and accelerated repopulation of peritoneal and omental macrophages by administration of Freund’s adjuvant. Cell Tissue Res 280:189–196PubMedGoogle Scholar
- Nolte M (2002) Compartments, cells and molecules in the spleen, Academic thesis. Vrije Universiteit medical center, AmsterdamGoogle Scholar
- Platt N, Suzuki H, Kurihara Y, Kodama T, Gordon S (1996) Role for the class A macrophage scavenger receptor in the phagocytosis of apoptotic thymocytes in vitro. Proceedings of the National Academy of Sciences of the United States of America 93:12456–12460Google Scholar
- van Rooijen N, Claassen E, Kraal G, Dijkstra CD (1989a) Cytological basis of immune functions of the spleen. Immunocytochemical characterization of lymphoid and non-lymphoid cells involved in the ’in situ’ immune response. Prog Histochem Cyto-chem 19:1–71Google Scholar