Bacterial communities in uranium mining waste piles and their interaction with heavy metals
High diversity and significant differences were found in the structures of bacterial communities present in several U mill tailings and U mining waste piles. Many bacterial strains were successfully cultured from those uranium wastes, most of which are unusually effective in different biotransformations of U. The molecular basis for the selective and reversible binding of U and some other toxic metals by one of the natural bacterial isolates was found to be a novel kind of S-layer protein. Our analysis indicates that uranium wastes are a valuable reservoir for unusual microorganisms prospective for bacteria-based bioremediation.
KeywordsUranium Radionuclide Bacillus Pseudomonas Trench
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- Felsenstein J (1993) PHYLIP (Phylogeny Inference Package) Version 3.5c Department of Genetics, University of Washington, Seatle.Google Scholar
- Goebel BM, Stackebrandt E (1994) Cultural and phylogenetic analysis of mixed microbial populations found in natural and commercial bioleaching environments. Appl Environ Microbiol 60: 1614-1621Google Scholar
- Ivanova I A, Stephen JR, Chang Y-J, Brüggemann J, Long PEL, McKinley JP, Kowalchuk GA, White DC (2000) A survey of 16S rRNA and amoA genes related to autotrophic ammonia-oxidizing bacteria of the ß-subdivision of the class proteobacteria in contaminated ground water. Can J Microbiol 46: 1012-1020Google Scholar
- Kato C, Li L, Nogi Y, Nakamura Y, Tamaoka J, Horikoshi K.. 1998. Extremely barophilic bacteria isolated from the Mariana Trench Challenger Deep at a depth of 11000 meters. Appl Environ Microbiol 64: 1510-1513Google Scholar
- Raff J (2002) PhD Thesis, University of Leipzig; March 2002Google Scholar
- Selenska-Pobell S (1995) Direct and simultaneous extraction of DNA and RNA from soil. In: Akkermans ADL, van Elsas JD, De Bruijn FJ, eds Molecular Microbial Ecology Manual 1.5.1. Dordrecht Netherlands: Kluwer Academic Publishers pp. 1-17Google Scholar
- Selenska-Pobell S, Miteva V, Boudakov I, Panak P, Bernhard G, and Nitsche H (1999) Selective accumulation of heavy metals by three indigenous Bacillus isolates, B. cereus, B. megaterium and B. sphaericus in drain waters from a uranium waste pile. FEMS Microbiol Ecology 29: 59-67CrossRefGoogle Scholar
- Selenska-Pobell S (2002) Diversity and activity of bacteria in uranium waste piles, In: Keith-Roach M & Livens F, ed. Interactions of Microorganisms with Radionuclides. Oxford, UK; Elsevier Sciences; pp. 225-253Google Scholar
- Takai, K, Moser D P, Onstott T C, Spoelstra N, Pfìffher S M, Dohnalkova AFredrickson, JK (2001) Alkaliphilus transvaalensis gen. nov., sp. nov., an extremely alkaliphilic bacterium isolated from a deep South African gold mine. Int J Syst Evol Microbiol 51: 1245-1256Google Scholar
- Tzvetkova I, Tzvetkova Tz, Groudeva V, Selenska-Pobell S (2001) Bacteria cultured from soil samples of uranium mining waste piles. In: Fanghaenel T, ed. FZR-Report-318: p. 39Google Scholar