Detection Based on Plasmon Resonance Energy Transfer

  • Yi-Tao Long
  • Chao Jing
Part of the SpringerBriefs in Molecular Science book series (BRIEFSMOLECULAR)


For nanoparticles with adsorbed chromophores, when the absorption bands of chromophores are overlapped with the resonance scattering bands of particles, “plasmon resonance energy transfer” (PRET) from metal nanoparticles to the surface-modified chromophores occurs. PRET enhances the sensitivity of absorption signals of chromophores with several orders of magnitudes. In this chapter, we discuss the discovery of PRET as well as its applications in ultrasensitive sensors.


Plasmon resonance energy transfer Chromophores Absorption spectroscopy Cytochrome c Cellular imaging Heavy metal ions 


  1. 1.
    Bruchez M, Moronne M, Gin P, Weiss S, Alivisatos AP (1998) Semiconductor nanocrystals as fluorescent biological labels. Science 281:2013–2016CrossRefGoogle Scholar
  2. 2.
    Cai L, Friedman N, Xie XS (2006) Stochastic protein expression in individual cells at the single molecule level. Nature 440:358–362CrossRefGoogle Scholar
  3. 3.
    Carlo DD, Lee LP (2006) Dynamic single-cell analysis for quantitative biology. Anal Chem 78:7918–7925CrossRefGoogle Scholar
  4. 4.
    Sun Y-P, Zhou B, Lin Y, Wang W, Fernando KS et al (2006) Quantum-sized carbon dots for bright and colorful photoluminescence. J Am Chem Soc 128:7756–7757CrossRefGoogle Scholar
  5. 5.
    Yu J, Xiao J, Ren X, Lao K, Xie XS (2006) Probing gene expression in live cells one protein molecule at a time. Science 311:1600–1603 CrossRefGoogle Scholar
  6. 6.
    Augspurger AE, Stender AS, Han R, Fang N (2014) Detecting plasmon resonance energy transfer with differential interference contrast microscopy. Anal Chem 86:1196–1201Google Scholar
  7. 7.
    Liu GL, Long YT, Choi Y, Kang T, Lee LP (2007) Quantized plasmon quenching dips nanospectroscopy via plasmon resonance energy transfer. Nat Methods 4:1015–1017CrossRefGoogle Scholar
  8. 8.
    Nie SM, Emory SR (1997) Probing single molecules and single nanoparticles by surface-enhanced Raman scattering. Science 275:1102–1106Google Scholar
  9. 9.
    Futamata M, Maruyama Y, Ishikawa M (2004) Adsorbed sites of individual molecules on Ag nanoparticles in single molecules sensitivity surface-enhanced Raman scattering. J Phys Chem B 108:13119–13127Google Scholar
  10. 10.
    Das P, Metiu H (1985) Enhancement of molecular fluorescence and photochemistry by small metal particles. J Phys Chem 89:4680–4687 Google Scholar
  11. 11.
    Andrew P, Barnes W (2004) Energy transfer across a metal film mediated by surface plasmon polaritons. Science 306:1002–1005CrossRefGoogle Scholar
  12. 12.
    Boussaad S, Pean J, Tao N (2000) High-resolution multiwavelength surface plasmon resonance spectroscopy for probing conformational and electronic changes in redox proteins. Anal Chem 72:222–226CrossRefGoogle Scholar
  13. 13.
    Haes AJ, Zou S, Zhao J, Schatz GC, Van Duyne RP (2006) Localized surface plasmon resonance spectroscopy near molecular resonances. J Am Chem Soc 128:10905–10914CrossRefGoogle Scholar
  14. 14.
    Choi Y, Kang T, Lee LP (2009) Plasmon resonance energy transfer (PRET)-based molecular imaging of cytochrome c in living cells. Nano Lett 9:85–90CrossRefGoogle Scholar
  15. 15.
    Choi Y, Park Y, Kang T, Lee LP (2009) Selective and sensitive detection of metal ions by plasmonic resonance energy transfer-based nanospectroscopy. Nat Nanotechnol 4:742–746CrossRefGoogle Scholar

Copyright information

© The Author(s) 2014

Authors and Affiliations

  1. 1.Key Laboratory for Advanced Materials and Department of ChemistryEast China University of Science and TechnologyShanghaiChina

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