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The Gentianaceae - Volume 2: Biotechnology and Applications pp 163–185Cite as

Protoplast Culture and Somatic Cell Hybridization of Gentians

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Abstract

During the last three decades, less than fifteen papers have described the results of scientific investigations in the field of gentian protoplast technology and somatic hybridization. Despite rather limited research already done on this subject, several important goals have been achieved. Protoplast-to-plant systems have been developed either for leading ornamental species or for specific medicinal plants. Two major protoplast sources were evaluated in gentians, namely differentiated leaf mesophyll cells and undifferentiated callus/cell suspensions. Plant regeneration proceeded by the two different pathways of shoot organogenesis or somatic embryogenesis. Some examples of somaclonal variation at the ploidy level were demonstrated within the pool of protoplast-derived regenerants. Totipotency exhibited by gentian protoplasts was exploited to create three different somatic hybrid combinations: intergeneric Swertia mussotii (+) Bupleurum scorzonerifolium , and interspecific Gentiana kurroo (+) G.  cruciata and G. cruciata (+) G. tibetica.

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Authors and Affiliations

  1. Polish Academy of Sciences, Botanical Garden—Center for Biological Diversity Conservation in Powsin, Prawdziwka 2, 02-973, Warsaw, Poland

    Karolina Tomiczak, Anna Mikuła & Jan J. Rybczyński

Authors
  1. Karolina Tomiczak
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  2. Anna Mikuła
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  3. Jan J. Rybczyński
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Correspondence to Karolina Tomiczak .

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Editors and Affiliations

  1. Botanical Garden-Center for Biological Diversity Conservation, Polish Academy of Sciences, Warsaw, Poland

    Jan J. Rybczyński

  2. Plant and Crop Sciences Division, University of Nottingham School of Biosciences, Loughborough, United Kingdom

    Michael R. Davey

  3. Botanical Garden-Center for Biological Diversity Conservation, Polish Academy of Sciences, Warsaw, Poland

    Anna Mikuła

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Tomiczak, K., Mikuła, A., Rybczyński, J.J. (2015). Protoplast Culture and Somatic Cell Hybridization of Gentians. In: Rybczyński, J., Davey, M., Mikuła, A. (eds) The Gentianaceae - Volume 2: Biotechnology and Applications. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-54102-5_7

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