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The Effect of Long-Wavelenght UV on BrUra substituted E. coli: Correlation between Survival, Strand Breaks, and BrUra-Incorporation

  • B. Seidler
  • W. Köhnlein
Conference paper

Abstract

If normal E. coli cells are irradiated with 260 nm UV the only photonproducts are thymine dimers (
$$\mathop {TT}\limits^ \wedge $$
). Using BrUra substituted cells DNA single strand breaks are induced as well via a debromination process. Carrier and Setlow (1972) have observed that the relative efficiency per BrUra of producing single strand breaks is decreasing with increasing BrUra substitution, indicating intramolecular energy transfer. Using (313) no such energy transfer should take place, and one would expect a rather simple correlation between loss of biological function and number of strand breaks in the LNA molecule, since under these conditions the amount of
$$\mathop {TT}\limits^ \wedge $$
produced, which are lethal for the cell, is negligible.

Keywords

Strand Break Relative Efficiency Coli Cell Single Strand Break Generation Growth 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Springer-Verlag Berlin Heidelberg 1979

Authors and Affiliations

  • B. Seidler
    • 1
  • W. Köhnlein
    • 1
  1. 1.Institut für StrahlenbiologieWestfälischen Wilhelms-UniversitätMünsterGermany

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