Bromodeoxyuridine Labelling: A Novel Biomarker of Colon Cancer Risk
Autoradiography following 3H-Thymidine (3H-T) incorporation into mucosal fragments in vitro has been widely employed in the study of cell proliferation in subjects at increased risk for colonic neoplasia . Using this technique significant differences in the labelling index (LI) and distribution of proliferative cells within large-bowel crypts have been described in hereditary non-polyposis colon cancer , familial polyposis  and in the presence of sporadic polyps or carcinoma . In vitro 3H-T labelling has therefore been proposed as a potential method for identification of high risk subjects , and has also been used as an “intermediate biomarker” of colon cancer risk in assessing the short-term effects of dietary intervention in man . However it is an exacting technique which requires the use of radioactive isotopes and significant delays (often several weeks) whilst autoradiographs are developed.
KeywordsReference Distribution Familial Polyposis Cell Position Colon Cancer Risk Proliferative Compartment
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