Bromodeoxyuridine Labelling: A Novel Biomarker of Colon Cancer Risk
Autoradiography following 3H-Thymidine (3H-T) incorporation into mucosal fragments in vitro has been widely employed in the study of cell proliferation in subjects at increased risk for colonic neoplasia . Using this technique significant differences in the labelling index (LI) and distribution of proliferative cells within large-bowel crypts have been described in hereditary non-polyposis colon cancer , familial polyposis  and in the presence of sporadic polyps or carcinoma . In vitro 3H-T labelling has therefore been proposed as a potential method for identification of high risk subjects , and has also been used as an “intermediate biomarker” of colon cancer risk in assessing the short-term effects of dietary intervention in man . However it is an exacting technique which requires the use of radioactive isotopes and significant delays (often several weeks) whilst autoradiographs are developed.
KeywordsAdenoma Radioactive Isotope Barium Luminal Paraffin
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