Interleukin — 8 and Related Cytokines

  • M. Baggiolini
  • B. Moser
Conference paper


Our understanding of the pathophysiology of neutrophil recruitment into diseased tissues is based on Metschnikoff’s concept of inflammation as a protective process, in which neutrophils act as scavengers of microorganisms and other unwanted material. Neutrophil enzymes (Metschnikoff’s cytases) bring about the intracellular digestion, but also lead to tissue damage when they escape from the phagocytic vacuoles. The study of phagocyte activation and migration became possible with the identification of chemotactic agonists. The existence of tissue-derived chemoattractants was postulated for quite some time, and cytokines and other cell-derived factors with apparent chemotactic properties were studied extensively (reviewed by Baggiolini et al 1992). The discovery of a novel neutrophil-activating protein, which is now called interleukin-8 (IL-8), and of several related cytokines (Baggiolini et al 1989, Baggiolini and Sorg 1992) has greatly widened our understanding of the mechanisms of neutrophil immigration into infected or otherwise injured tissues.


Bordetella Pertussis Related Cytokine Palmoplantar Pustulosis Neutrophil Chemotactic Factor Phagocytic Vacuole 
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  1. Anttila HSI, Reitamo S, Erkko P, Ceska M, Moser B, Baggiolini M (1992) Interleukin-8 immunoreactivity in the skin of healthy subjects and patients with palmoplantar pustulosis and psoriasis. J Invest Dermatol 98:96–101CrossRefPubMedGoogle Scholar
  2. Baggiolini M, Dewald B, Walz A (1992) NAP-l/IL-8 and related chemotactic cytokines. In: Inflammation, Basic Principles and Clinical Correlates, 2nd Edition. Gallin JI, Goldstein, IM, Snyderman, R, eds,, Raven Press New York, in pressGoogle Scholar
  3. Baggiolini M, Sorg C (1992) Interleukin-8 (NAP-1) and related chemotactic cytokines. Cytokine vol 4 Karger, Basel, p 164Google Scholar
  4. Baggiolini M, Walz A, Kunkel SL (1989) Neutrophil-activating peptide-1/interleukin 8, a novel cytokine that activates neutrophils. J Clin Invest 84:1045–1049.PubMedCentralCrossRefPubMedGoogle Scholar
  5. Bazzoni F, Cassatella MA, Rossi F, Ceska M, Dewald B, Baggiolini M (1991) Phagocytosing neutrophils produce and release high amounts of the neutrophil-activating peptide 1/interkeukin 8. J Exp Med 173:771–774.CrossRefPubMedGoogle Scholar
  6. Boulay F, Tardif M, Brouchon L, Vignais P (1990) Synthesis and use of a novel N-formyl peptide derivative to isolate a human N-formyl peptide receptor cDNA. Biochem Biophys Res Commun 168:1103–1109CrossRefPubMedGoogle Scholar
  7. Brennan FM, Zachariae COC, Chantry D, Larsen CG, Turner M, Maini RN, Matsushima K, Feldmann M (1990) Detection of interleukin 8 biological activity in synovial fluids from patients with rheumatoid arthritis and production of interleukin 8 mRNA by isolated synovial cells. Eur J Immunol 20:2141–2144CrossRefPubMedGoogle Scholar
  8. Carveth HJ, Bohnsack JF, McIntyre TM, Baggiolini M, Prescott SM, Zimmerman GA (1989) Neutrophil activating factor (NAF) induces polymorphonuclear leukocyte adherence to endothelial cells and to subendothelial matrix proteins. Biochem Biophys Res Commun 162:387–393.CrossRefPubMedGoogle Scholar
  9. Clark-Lewis I, Schumacher C, Baggiolini M, Moser B (1991) Structure-activity relations of interleukin 8 determined using chemically synthesized analogs: Critical role of N-terminal residues and evidence for uncoupling of Chemotaxis, exocytosis an receptor binding activities. J Biol Chem 266:23128–23134PubMedGoogle Scholar
  10. Colditz I, Zwahlen R, Dewald B, Baggiolini M (1989) In vivo inflammatory activity of neutrophil-activating factor, a novel chemotactic peptide derived from human monocytes. Am J Pathol 134:755–760.PubMedGoogle Scholar
  11. Detmers PA, Lo SK, Olsen-Egbert E, Walz A, Baggiolini M, Cohn ZA (1990) Neutrophil-activating protein 1/interleukin 8 stimulates the binding activity of the leukocyte adhesion receptor CDllb/CD18 on human neutrophils. J Exp Med 171:1155–1162.CrossRefPubMedGoogle Scholar
  12. Gillitzer R, Berger R, Mielke, V Müller C, Wolff K, Stingl G (1991) Upper keratinocytes of psoriatic skin lesions express high levels of NAP-l/IL-8 mRNA in situ. J Invest Dermatol 97:73–79CrossRefPubMedGoogle Scholar
  13. Golds EE, Mason P, Nyirkos P (1989) Inflammatory cytokines induce synthesis and secretion of gro protein and a neutrophil chemotactic factor but not 32-microglobulin in human synovial cells and fibroblasts. Biochem J 259:585–588PubMedGoogle Scholar
  14. Haskill S, Peace A, Morris J, Sporn, S.A., Anisowicz, A., Lee, S.W., Smith, T., Martin, G., Ralph, R, Sager, R (1990) Identification of three related human GRO genes encoding cytokine functions. Proc Natl Acad Sci USA 87:7732–7736.CrossRefPubMedGoogle Scholar
  15. Hébert CA, Vitangcol RV, Baker JB (1991) Scanning mutagenesis of interleukin-8 identifies a cluster of residues required for receptor binding. J Biol Chem 266:18989–18994PubMedGoogle Scholar
  16. Holmes WE, Lee J, Kuang WJ, Rice GC, Wood WI (1991) Structure and functional expression of a human interleukin-8 receptor. Science 253:1278–1280CrossRefPubMedGoogle Scholar
  17. Larsen CG, Anderson AO, Appella E, Oppenheim JJ, Matsushima K (1989) The neutrophil-activating protein (NAP-1) is also chemotactic for T lymphocytes. Science 243:1464–1466.CrossRefPubMedGoogle Scholar
  18. Leonard EJ, Yoshimura T, Tanaka S, Raffeld M (1991) Neutrophil recruitment by intradermally injected neutrophil attractant/activation protein-1. J Invest Dermatol 96:690–694.CrossRefPubMedGoogle Scholar
  19. Leonard, EJ, Yoshimura, T (1990) Neutrophil attractant/activation protein-1 (NAP-1 (interleukin-8)) Am J Respir Cell Mol Biol 2:479–486CrossRefGoogle Scholar
  20. Lindley I, Aschauer H, Scifert JM, Lam C, Brunowsky W, Kownatzki E, Thelen M, Peveri P, Dewald B, von Tscharner V, Walz A, Baggiolini M. (1988) Synthesis and expression in Escherichia coli of the gene encoding monocyte-derived neutrophil-activating factor: Biological equivalence between natural and recombinant neutrophil-activating factor. Proc Natl Acad Sci U S A 85:9199–9203.PubMedCentralCrossRefPubMedGoogle Scholar
  21. Modi WS, Dean M, Seuanez HN, Mukaida N, Matsushima K, O’Brien SJ (1990) Monocyte-derived neutrophil chemotactic factor (MDNCF/IL-8) resides in a gene cluster along with several other members of the platelet factor 4 gene superfamily. Hum Genet 84:185–187.CrossRefPubMedGoogle Scholar
  22. Moser B, Clark-Lewis I, Zwahlen R, Baggiolini M (1990) Neutrophil-activating properties of the melanoma growth-stimulatory activity. J Exp Med 171:1797–1802.CrossRefPubMedGoogle Scholar
  23. Moser B, Schumacher C, von Tscharner V, Clark-Lewis I, Baggiolini M (1991) Neutrophil-activating peptide 2 and gro/melanoma growth-stimulatory activity interact with neutrophil-activating peptide 1/interleukin 8 receptors on human neutrophils. J Biol Chem 266:10666–10671.PubMedGoogle Scholar
  24. Murphy PM, Tiffany HL (1991) Cloning of complementary DNA encoding a functional human interleukin-8 receptor. Science 253:1280–1283CrossRefPubMedGoogle Scholar
  25. Nickoloff BJ, Karabin GD, Barker JNWN, Griffith CEM, Sarma V, Mitra RS, Elder JT, Kunkel SL, Dixit VM (1991) Cellular localization of interleukin-8 and its inducer, tumor necrosis factor-α in psoriasis. Am J Pathol 138:129–140PubMedGoogle Scholar
  26. Paccaud J-P, Schifferli JA, Baggiolini M (1990) NAP-l/IL-8 induces upregulation of CR1 receptors in human neutrophil leukocytes. Biochem Biophys Res Commun 166:187–192.CrossRefPubMedGoogle Scholar
  27. Peveri P, Walz A, Dewald B, Baggiolini M (1988) A novel neutrophil-activating factor produced by human mononuclear phagocytes. J Exp Med 167:1547–1559.CrossRefPubMedGoogle Scholar
  28. Richmond A, Baientien E, Thomas HG, Flaggs, G., Barton, D.E., Spiess, J., Bordoni, R., Francke, U (1988) and Derynck, R. Molecular characterization and chromosomal mapping of melanoma growth stimulatory activity, a growth factor structurally related to ß-thromboglobulin. EMBO J 7:2025–2033.PubMedGoogle Scholar
  29. Sager R, Anisowicz A, Pike MC, Beckmann P, Smith T (1992) Structural, regulatory, and functional studies of the GRO gene and protein. In Cytokines Vol.4. Interleukin-8 (NAP-1) and related chemotactic cytokines. Baggiolini M, Sorg C, eds, Karger, Basel 96–116.Google Scholar
  30. Scitz M, Dewald B, Gerber N, Baggiolini M. (1991) Enhanced production of neutrophil-activating peptide-1/interleukin-8 in rheumatoid arthritis. J Clin Invest 87:463–469.CrossRefGoogle Scholar
  31. Sticherling M, Bornscheuer E, Schröder JM, Christophers E (1991) Localization of neutrophil-activating peptide-1/interleukin-8 -immunoreactivity in normal and psoriatic skin. J Invest Dermatol 96:26–30CrossRefPubMedGoogle Scholar
  32. Van Damme J, Bunning RAD, Conings R, Graham R, Russell G, Opdenakker G (1990) Characterization of granulocyte chemotactic activity from human cytokine-stimulated chondrocytes as interleukin 8. Cytokine 2:106–111CrossRefPubMedGoogle Scholar
  33. Walz A, Baggiolini M (1990) Generation of the neutrophil-activating peptide NAP-2 from platelet basic protein or connective tissue-activating peptide III through monocyte proteases. J Exp Med 171:449–454.CrossRefPubMedGoogle Scholar
  34. Walz A, Burgener R, Car B, Baggiolini M, Kunkel SL, Strieter RM (1991) Structure and neutrophil-activating properties of a novel inflammatory peptide (ENA-78) with homology to IL-8. J Exp Med 174:1355–1362CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • M. Baggiolini
    • 1
  • B. Moser
    • 1
  1. 1.Theodor - Kocher InstituteUniversity of BerneSwitzerland

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