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Evaluation of the Coordinated Control of Activated Glycolysis and Respiration by Orthophosphate and ADP in Ascites Tumor Cells

  • E. L. Coe
  • Mary H. Coe
  • In-Young Lee
Conference paper

Abstract

During the first minute after addition of glucose to respiring ascites tumor cells, there are two internally regulated glycolytic units: hexokinase through phospho-fructokinase; and triose-P dehydrogenase through lactate dehydrogenase. The velocity of the former, V PFK, and of the latter V LDH, as well as the velocities of respiration and ATP hydrolysis are linear functions of both ADP and intracellular Pi. Expressions for d/dt(ADP) and d/dt(Pi) are solved for ADP, assuming various combinations of ADP and Pi dependence for the velocities. Comparisons of theoretical and experimental ADP curves indicate that neither ADP nor Pi control both V PFK and V LDH, but that Pi probably controls V PFK while ADP controls V LDH.

Keywords

Lactate Dehydrogenase Glucose Addition Triose Phosphate Glucose Equivalent Intermediate Segment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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    Levinson, C.: Biochim. Biophys. Acta 120, 292 (1966).PubMedCrossRefGoogle Scholar
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    Maitra, P. K., and B. Chance: In: B. Chance, R. W. Estabrook and J. R. Williamson (Eds.) Control of Energy Metabolism, p. 157. New York: Academic Press 1965.Google Scholar

Copyright information

© Springer-Verlag Berlin · Heidelberg 1968

Authors and Affiliations

  • E. L. Coe
  • Mary H. Coe
  • In-Young Lee

There are no affiliations available

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