Evaluation of the Coordinated Control of Activated Glycolysis and Respiration by Orthophosphate and ADP in Ascites Tumor Cells
During the first minute after addition of glucose to respiring ascites tumor cells, there are two internally regulated glycolytic units: hexokinase through phospho-fructokinase; and triose-P dehydrogenase through lactate dehydrogenase. The velocity of the former, V PFK, and of the latter V LDH, as well as the velocities of respiration and ATP hydrolysis are linear functions of both ADP and intracellular Pi. Expressions for d/dt(ADP) and d/dt(Pi) are solved for ADP, assuming various combinations of ADP and Pi dependence for the velocities. Comparisons of theoretical and experimental ADP curves indicate that neither ADP nor Pi control both V PFK and V LDH, but that Pi probably controls V PFK while ADP controls V LDH.
KeywordsHydrolysis Lactate Respiration Fructose Diphosphate
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