For the examination of extended specimens such as ribbons of serial sections, thin metal films and some replicas in the electron microscope, it is desirable that the field of view should not be obstructed by grid bars. Suitable supporting substrates must satisfy five essential conditions.
  1. a)

    They should be easily prepared and mounted over holes of at least 0.5 mm diameter.

  2. b)

    Under intense electron bombardment they should not contribute to specimen shrinkage or movement.

  3. c)

    They must not break under the strains imposed in picking up floating specimens and subsequently drying them.

  4. d)

    Their area density should be very low.

  5. e)

    They must be clean and smooth.



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    Farrant, J. L., and S. E. Powell: Proc. First Regional Conf. in Asia and Oceania. p. 147. Tokyo 1956.Google Scholar
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    Sugata, E., and Y. Nishitani: Technol. Rep. Osaka Univ. 4, No. 101 (1954).Google Scholar

Copyright information

© Springer-Verlag OHG. Berlin · Göttingen · Heidelberg 1960

Authors and Affiliations

  • W. C. T. Dowell
    • 1
  • M. S. C. Birbeck
    • 2
  • E. Sugata
    • 3
  • H. Yokoya
    • 3
  • K. Kitakaze
    • 3
  1. 1.Division of Chemical Physics, Chemical Research LaboratoriesCommonwealth Scientific and Industrial Research OrganizationMelbourneAustralia
  2. 2.Chester Beatty Research Institute (Institute of Cancer Research: Royal Cancer Hospital)London, S. W. 3UK
  3. 3.Osaka UniversityOsakaJapan

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