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Isotopes in studies with antimetabolites

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Radioactive Isotopes in Physiology Diagnostics and Therapy / Künstliche Radioaktive Isotope in Physiologie Diagnostik und Therapie

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Abstract

The concept of inhibition of growth of cells by an organic compound having specific structural features which would cause it to bind to a cell surface or protein molecule was introduced by Ehrlich in the late nineteenth century. However the concept of the antimetabolite, i e inhibition of a biochemical reaction by a compound similar in structure to a metabolite because of competition for a site on an enzyme molecule, has developed during the past twenty years. It is noteworthy that this development closely parallels the development of use of isotopes in the study of biochemical problems. That the two areas have not grown in different directions is evident from a survey of articles in any recent biochemical journal. Many investigators have used a combination of radioactive isotopes and anti-metabolites to gain information about biochemical mechanisms. It is, perhaps, in this area that the use of isotopes in the study of antimetabolites has achieved its greatest importance. It is the purpose of this chapter to point out how these concepts have been brought to bear in gaining information about a number of specific metabolic reactions. The choice of reactions and examples is dictated by personal interest, but the principles involved would be the same for an entirely different group of examples.

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  1. S. B. Binkley
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H. Schwiegk F. Turba

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Binkley, S.B. (1961). Isotopes in studies with antimetabolites. In: Schwiegk, H., Turba, F. (eds) Radioactive Isotopes in Physiology Diagnostics and Therapy / Künstliche Radioaktive Isotope in Physiologie Diagnostik und Therapie. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-49762-9_32

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  • DOI: https://doi.org/10.1007/978-3-642-49762-9_32

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