Abstract
In the last few years recent studies implicating a deficiency of interleukins (ILs) in several diseases have underlined the importance of measuring in vitro both DNA synthesis and cytokine production (IL-2, IL-6) in the same cell system [1–3]. The production of ILs by peripheral blood mononuclear cells (PBMC) in vitro can be induced by mitogens, phorbol ester, or ionophores [4, 5]. Many different protocols exist that propose optimal conditions for IL-2 or IL-6 induction [1, 4–7].
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© 1992 Springer-Verlag, Berlin Heidelberg
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Reinhold, D., Bank, U., Ansorge, S. (1992). Pokeweed Mitogen Induction of Interleukin-2 and Interleukin-6 in Mononuclear Cells: An Alternative Approach to the Combined Phytohemagglutinin/Phorbol Myristrate Acetate Stimulation In Vitro. In: Freund, M., Link, H., Schmidt, R.E., Welte, K. (eds) Cytokines in Hemopoiesis, Oncology, and AIDS II. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-48715-6_47
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DOI: https://doi.org/10.1007/978-3-642-48715-6_47
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