Variation Analysis of Seven LightCycler Based Real-Time PCR Systems to Detect Genetically Modified Products (RRS, Bt176, Bt11, Mon810, T25, Lectin, Invertase)

Abstract

Foodstuffs, additives, and processing aids which comprise or are derived from more than 1% of genetically modified organisms (GMOs) have to be labeled according to Swiss [1] and EU law [2–4] as GMO products. As cultivation of GMOs is increasing rapidly and the products composed of GMO plants appear more and more on the world market, there is a need for sensitive, specific, and quick detection methods in order to monitor the labeling of foodstuffs. Several qualitative [5–7] as well as quantitative-competitive [8–10] PCR methods already exist. However, quantitative real-time PCR methods, which no longer require internal standards, competitors, time-consuming PCR reactions, and gel electrophoresis have recently appeared [11]. One such system is LightCycler real-time PCR technology. Real-time PCR systems allow the analysis of the samples while amplification is still in progress. Results can be obtained in less than 1 h.