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Discussion Methods and Criteria of Mammalian Cell Synchrony

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Normal and Malignant Cell Growth

Abstract

The sequence of events occurring between one cell division and the next, i.e., during the cell generation cycle, and the relationship between these different events is of primary importance to our understanding of the proliferation process in cells and perhaps also to the distinctions between normal and malignant cells.

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References

  1. Belli, J. A.: Effect of temperature on the radiation response of synchronous mammalian cells in culture. Rad. Res. 25, 174 (1965).

    Google Scholar 

  2. Blumenthal, L. K. and Zaler, S. A.: Index for measurement of synchronization of cell populations. Science 135, 724 (1962).

    Article  PubMed  CAS  Google Scholar 

  3. Bootsma, D., Budke, L. and Vos, O.: Studies on synchronous division of tissue culture cells initiated by excess thymidine. Exp. Cell Res. 33, 301 (1964).

    Article  PubMed  CAS  Google Scholar 

  4. Burns, V. W.: Cell division synchronization. Progr. Biophysics and Biophysic. Chem. 12, 1 (1962).

    CAS  Google Scholar 

  5. Djordjevic, E. and Tolmach, L. J.: X-ray sensitivity of HeLa S3 cells in the G2 phase: Comparison of two methods of synchronization. Biophys. 7, 77 (1967).

    Article  CAS  Google Scholar 

  6. Engleberg, J.: The decay of synchronization of cell division. Exp. Cell Res. 36, 647 (1964).

    Article  Google Scholar 

  7. Engleberg, J.: A method of measuring the degree of synchronization of cell populations. Exp. Cell Res. 23, 218 (1961).

    Article  Google Scholar 

  8. Erikson, R. L. and Szybalski, W.: Molecular radiobiology of human cell lines. IV. Variation in ultraviolet light and X-ray sensitivity during the division cycle. Rad. Res. 18, 200 (1963).

    Article  Google Scholar 

  9. Kim, J. H. and Stambuk, B. K.: Synchronization of HeLa cells by vinblastine sulfate. Exp. Cell Res. 44, 631 (1966).

    Article  PubMed  CAS  Google Scholar 

  10. Kubitschek, H. E.: Discrete distributions of generation-rate. Nature 195, 350 (1962).

    Article  PubMed  CAS  Google Scholar 

  11. Littlefield, J. W.: DNA synthesis in partially synchronized L cells. Exp. Cell Res. 26, 318 (1962).

    Article  PubMed  CAS  Google Scholar 

  12. Newton, A. A. and Wildy, P.: Parasynchronous division of HeLa cells. Exp. Cell Res. 16, 724 (1959).

    Article  Google Scholar 

  13. Petersen, D. F. and Anderson, E. C.: Quantity production of synchronized mammalian cells in suspension culture. Nature 203, 642 (1964).

    Article  PubMed  CAS  Google Scholar 

  14. Pfeiffer, S. E. and Tolmach, L. J.: Selecting synchronous populations of mammalian cells. Nature 123, 139 (1967).

    Article  Google Scholar 

  15. Puck, T. T.: Phasing, mitotic delay and chromosomal aberrations in mammalian cells. Science 144, 565 (1964).

    Article  PubMed  CAS  Google Scholar 

  16. Robbins, E. and Marcus, P. L.: Mitotically synchronized mammalian cells: A simple method of obtaining large populations. Science 144, 1152 (1964).

    Article  PubMed  CAS  Google Scholar 

  17. Rueckert, R. R. and Mueller, G. C.: Studies on unbalanced growth in tissue culture. I. Induction and consequences of thymidine deficiency. Cancer Res. 20, 1584 (1960).

    PubMed  CAS  Google Scholar 

  18. Schindler, R.: Biochemical studies of the division cycle of mammalian cells: Evidence for the premitotic period. Biochem. Pharmacol. 12, 533 (1963).

    Article  PubMed  CAS  Google Scholar 

  19. Sinclair, W. K.: Hydroxyurea: Differential lethal effects on cultured mammalian cells during the cell cycle. Science 150, 1729 (1965).

    Article  PubMed  CAS  Google Scholar 

  20. Sinclair, W. K.: Hydroxyurea: Effects on Chinese hamster cells grown in culture. Cancer Res., Part I, 297 (1967).

    Google Scholar 

  21. Sinclair, W. K. and Morton, R. A.: X-ray sensitivity during the cell generation cycle of cultured Chinese hamster cells. Rad. Res. 29, 450 (1966).

    Article  CAS  Google Scholar 

  22. Sinclair, W. K. and Morton, R. A.: X-ray and ultra-violet sensitivity of synchronized Chinese hamster cells at various stages of the cell cycle. Biophys. J. 5, 1 (1965).

    Article  PubMed  CAS  Google Scholar 

  23. Sinclair, W. K. and Morton, R. A.: Variations in X-ray response during the division cycle of partially synchronized Chinese hamster cells in culture. Nature 199, 1158 (1963).

    Article  PubMed  CAS  Google Scholar 

  24. Stubblefield, E. and Klevecz, R.: Synchronization of Chinese hamster cells by reversal of colcemid inhibition. Exp. Cell Res. 40, 660 (1965).

    Article  PubMed  CAS  Google Scholar 

  25. Terasima, T. and Tolmach, L. J.: Growth and nucleic acid synthesis in synchronously dividing populations of HeLa cells. Exp. Cell Res. 30, 344 (1963).

    Article  PubMed  CAS  Google Scholar 

  26. Terasima, T. and Tolmach, L. J.: Variations in several responses in HeLa cells to X-irradiation during the division cycle. Biophys. J. 3, 11 (1963).

    Article  PubMed  CAS  Google Scholar 

  27. Till, J. E., Whitmore, G. F. and Gulyas, S.: Deoxyribonucleic acid synthesis in individual L-strain mouse cells. II. Effects of thymidine starvation. Biochim. Biophysica Acta 72, 277 (1963).

    Article  CAS  Google Scholar 

  28. Whitfield, J. F. and Youdale, T.: Synchronization of cell division in suspension cultures of L-strain mouse cells. Exp. Cell Res. 38, 208 (1965).

    Article  PubMed  CAS  Google Scholar 

  29. Whitmore, G. F. and Gulyas, S.: Synchronization of mammalian cells with tritiated thymidine. Science 151, 691 (1966).

    Article  PubMed  CAS  Google Scholar 

  30. Xeros, N.: Deoxyriboside control and synchronization of mitosis. Nature 194, 682 (1962).

    Article  PubMed  CAS  Google Scholar 

  31. Zeuthen, E.: Artificial and induced periodicity in living cells. Advances Biol. and Med. Physics 6, 37 (1958).

    CAS  Google Scholar 

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Sinclair, W.K. (1969). Discussion Methods and Criteria of Mammalian Cell Synchrony. In: Fry, R.J.M., Griem, M.L., Kirsten, W.H. (eds) Normal and Malignant Cell Growth. Recent Results in Cancer Research / Fortschritte der Krebsforschung / Progrès dans les recherches sur le cancer, vol 17. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-48263-2_11

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  • DOI: https://doi.org/10.1007/978-3-642-48263-2_11

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-48265-6

  • Online ISBN: 978-3-642-48263-2

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