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Plasmid DNA vaccination: mechanism of antigen presentation

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Gene Vaccination: Theory and Practice

Part of the book series: Principles and Practice ((PRINCIPLES))

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Abstract

Direct injection of naked plasmid DNA either intramuscularly or intradermally induces strong, long-lived immune responses to the antigen encoded by the gene vaccine. While the intradermal route of administration appears to be the most efficient, there is evidence that either route leads to production of antibody and the activation of major histocompatibility complex (MHC) class I-restricted, antigen-specific cytotoxic T lymphocytes (CTL) and MHC class II-restricted CD4+ T cells secreting Thl-type cytokines such as interferon-γ (IFN-γ) [1–9]. Plasmid DNA immunization has potential advantages compared to traditional protein vaccination due to the strong CTL and Thl responses induced, the prolonged antigen expression, and the resistance of the antigen source to antibody-mediated clearance. As a consequence, gene vaccination has potential applications in the fields of infectious diseases, allergy and cancer.

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Author information

Authors and Affiliations

  1. Department of Medicine and The Sam and Rose Stein Institute for Research on Aging, University of California, San Diego, Clinical Sciences Building, Room 126, 9500 Gilman Drive, La Jolla, CA, 92093-0663, USA

    Maripat Corr & Helen Tighe

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  1. Maripat Corr
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  2. Helen Tighe
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Editors and Affiliations

  1. Department of Medicine, University of California, San Diego, 9500 Gilman Drive, 92093-0663, La Jolla, CA, USA

    Eyal Raz M.D. (Associate Professor) (Associate Professor)

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© 1998 Springer-Verlag Berlin Heidelberg

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Corr, M., Tighe, H. (1998). Plasmid DNA vaccination: mechanism of antigen presentation. In: Raz, E. (eds) Gene Vaccination: Theory and Practice. Principles and Practice. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-46867-4_2

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  • DOI: https://doi.org/10.1007/978-3-642-46867-4_2

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-46869-8

  • Online ISBN: 978-3-642-46867-4

  • eBook Packages: Springer Book Archive

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