Abstract
Partial enzymatic filling-in of cohesive ends generated in phage DNAs by restriction endonucleases allows one to obtain fragments which are able to ligate only in a predetermined order, excluding the self-ligation of the vector. The same approach is applicable for prevention of self-ligation of the insert, too. Partial filling-in of cohensive ends has been successfully used to obtain genomic [1] and cDNA libraries.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Zabarovsky ER, Allikmets RL (1986) Gene 42 (1): 119 – 123
Zabarovsky ER, Demirov DG, Nurbekov MK, Kisselev LL (1986) Bioorgan Khimia (Moscow) 12 (8):1135 –1138
Author information
Authors and Affiliations
Rights and permissions
Copyright information
© 1987 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Zabarovsky, E.R., Nurbekov, M.K., Turina, O.V., Kisselev, L.L. (1987). Gene Cloning: Some Methodological Improvements. In: Organization and Function of the Eucaryotic Genome. Sitzungsberichte der Heidelberger Akademie der Wissenschaften, vol 1987/88 / 1987/3. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-46611-3_24
Download citation
DOI: https://doi.org/10.1007/978-3-642-46611-3_24
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-17798-2
Online ISBN: 978-3-642-46611-3
eBook Packages: Springer Book Archive