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The Assay of Neurohypophysial Hormones in Blood and Other Body Fluids

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Neurohypophysial Hormones and Similar Polypeptides

Abstract

This section will be mainly orientated around the detection and measurement of oxytocin and vasopressin at low concentrations, such as may occur naturally in blood or urine. The choice of method in this type of work is influenced more by considerations of high sensitivity, by specificity and by simplicity than by limits of error. This contrasts with requirements of methods for estimating the oxytocic potency of posterior pituitary glands, or for the investigation of synthetic homologues of oxytocin, when in general there is no practical limit to the amount of active substance available and hence the sensitivity of the method is not of great importance, whereas the reliability of the estimate is of very great importance indeed. Assays are discussed from this point of view, e. g. pharmacopoeial standardisation, in the chapter by Stürmer.

Methods are good if they are accurate, rapid and simple, and bad if they are inaccurate, slow and need skill. That accuracy is necessary all are agreed. That speed in obtaining results is important is well known... Few people recognise that methods are good in proportion as the technique is simple.

J.H. Burn (1937)

Biological Standardisation

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Authors
  1. R. J. Fitzpatrick
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  2. P. J. Bentley
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Editors and Affiliations

  1. Medizinisch-Biologische Forschungslaboratorien, Sandoz A.G., 4000, Basel, Switzerland

    B. Berde

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© 1968 Springer-Verlag Berlin Heidelberg

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Fitzpatrick, R.J., Bentley, P.J. (1968). The Assay of Neurohypophysial Hormones in Blood and Other Body Fluids. In: Berde, B. (eds) Neurohypophysial Hormones and Similar Polypeptides. Handbuch der experimentellen Pharmakologie / Handbook of Experimental Pharmacology, vol 23. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-46127-9_5

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  • DOI: https://doi.org/10.1007/978-3-642-46127-9_5

  • Publisher Name: Springer, Berlin, Heidelberg

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