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Biochemical and Autoradiographic Approaches to the Characterization of Adenosine Receptors in Brain

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Topics and Perspectives in Adenosine Research

Summary

The distribution and biochemical properties of adenosine receptors have been studied using radioligand-binding techniques. Using autoradiographic methods, the distributions of A1 and A2 receptors were found to be quite different. Whereas the A1 receptors are mostly concentrated in the hippocampus and cerebellum of rat brain, the A2 receptors are found mainly in the striatum, nucleus accumbens, and olfactory tubercle. The binding of ligands to A1 receptors is modified by several factors, including guanine nucleotides, sodium ions, adenine nucleotides, and Mg2+. Analysis of the actions of adenine nucleotides and Mg2+ indicates that these act at sites other than the guanine nucleotide regulatory site. These observations, together with structural studies using solubilized receptor or radiation inactivation, indicate the multimolecular nature of the A1 adenosine-receptor complex.

Financial support from the Deutsche Forschungsgemeinschaft [West German Science Foundation] is gratefully acknowledged.

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Author information

Authors and Affiliations

  1. Department of Neuromorphology, Max Planck Institute for Psychiatry, Am Klopferspitz 18a, 8033, Martinsried, Germany

    M. Reddington, S. P. Alexander, A. Erfurth, K. S. Lee & G. W. Kreutzberg

  2. Department of Anatomy, Thomas Jefferson University, 1020 Locust Street, Philadelphia, Pa, USA

    K. S. Lee

Authors
  1. M. Reddington
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  2. S. P. Alexander
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  3. A. Erfurth
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  4. K. S. Lee
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  5. G. W. Kreutzberg
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Editors and Affiliations

  1. Physiologisches Institut, Universität München, Pettenkoferstraße 12, 8000, München 2, Federal Republic of Germany

    Eckehart Gerlach  & Bernhard Friedrich Becker  & 

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© 1987 Springer-Verlag Berlin Heidelberg

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Reddington, M., Alexander, S.P., Erfurth, A., Lee, K.S., Kreutzberg, G.W. (1987). Biochemical and Autoradiographic Approaches to the Characterization of Adenosine Receptors in Brain. In: Gerlach, E., Becker, B.F. (eds) Topics and Perspectives in Adenosine Research. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-45619-0_5

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  • DOI: https://doi.org/10.1007/978-3-642-45619-0_5

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-45621-3

  • Online ISBN: 978-3-642-45619-0

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