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A Critique on the Use of Adenosine Deaminase to Test the Adenosine Hypothesis: Disregarded Implicit Assumptions

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Topics and Perspectives in Adenosine Research

Summary

The idea that intracoronary injection of adenosine deaminase should destroy interstitial fluid adenosine and abolish regulation of coronary blood flow has sparked a number of controversial studies. In some studies adenosine deaminase infusion failed to block vascular responses attributed to endogenous adenosine, but in others it depressed them. Such results are to be expected because the amount of adenosine deaminase calculated from direct measurements of enzyme kinetics (in the adenosine concentration range of 10−8-1(10−3 M) necessary to destroy adenosine in the myocardial interstitial fluid is considerably greater than that used by investigators who have used this approach.

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Author information

Authors and Affiliations

  1. Department of Physiology, University of Virginia School of Medicine, Charlottesville, VA, 22908, USA

    R. Rubio, R. M. Knabb, S. W. Ely & R. M. Berne

Authors
  1. R. Rubio
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  2. R. M. Knabb
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  3. S. W. Ely
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  4. R. M. Berne
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Editor information

Editors and Affiliations

  1. Physiologisches Institut, Universität München, Pettenkoferstraße 12, 8000, München 2, Federal Republic of Germany

    Eckehart Gerlach  & Bernhard Friedrich Becker  & 

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© 1987 Springer-Verlag Berlin Heidelberg

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Rubio, R., Knabb, R.M., Ely, S.W., Berne, R.M. (1987). A Critique on the Use of Adenosine Deaminase to Test the Adenosine Hypothesis: Disregarded Implicit Assumptions. In: Gerlach, E., Becker, B.F. (eds) Topics and Perspectives in Adenosine Research. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-45619-0_37

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  • DOI: https://doi.org/10.1007/978-3-642-45619-0_37

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-45621-3

  • Online ISBN: 978-3-642-45619-0

  • eBook Packages: Springer Book Archive

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