Abstract
Although the manual counting chamber (hemacytometer) is the gold standard for counting cells, this method is subject to great variability due to the “human factor.” The automated cell counter (Coulter Counter) can enumerate cells in less time and with greater accuracy than the hemacytometer by removing many of the steps in which errors are made. While the Coulter Counter (and others of its type) has been used for many years in the cell culture field, there have been few studies to validate its use with specific cell types. We conducted several experiments in which we assessed the accuracy of the Coulter Counter over counts made with a hemacytometer as well as validated its use for the counting of satellite cells and preadipocytes.
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Acknowledgments
This study was supported by the Washington State University Agricultural Research Center (Project 0913) and USDA NRICGP grant number 2002-35206-12880. The authors wish to acknowledge Adrienne Kinkle, Brena Holman, and Erika Thompson for assistance with this project. MVD is a member of the USDA Regional Research Project NC-131, “Molecular Mechanisms Regulating Skeletal Muscle Growth and Development.”
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Fernyhough-Culver, M., Helterline-Icenoggle, D.L., Vierck, J.L., Hill, R.A., Dodson, M.V. (2014). Cell, Tissue, and Organ Culture: Coulter Counter Use in the Enumeration of Muscle and Fat Stem Cells. In: Shiffman, M., Di Giuseppe, A., Bassetto, F. (eds) Stem Cells in Aesthetic Procedures. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-45207-9_19
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DOI: https://doi.org/10.1007/978-3-642-45207-9_19
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