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Introduction

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Abstract

Although the whole human genome was sequenced in 2001 and high-throughput genome sequencing technologies were greatly developed in recent years, our understanding of most of the physiological and pathological processes in biological systems is still limited. As all the biological functions are performed by proteins, it is essential to investigate the relationship of the protein expression level with different biological processes. Proteomics is aimed to elucidate all the proteins expressed within a biological system, which is much more complex than genomics for the reason that proteins are constituted by more than 20 types of amino acids and the expressed proteins can be dynamically modified by more than 300 types of posttranslational modifications, such as phosphorylation, glycosylation, acetylation, and methylation.

Keywords

  • Monolithic Column
  • Trap Column
  • Quantification Accuracy
  • Quantitative Proteome Analysis
  • Proteome Coverage

These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Fig. 1.1

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Correspondence to Fangjun Wang .

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Wang, F. (2014). Introduction. In: Applications of Monolithic Column and Isotope Dimethylation Labeling in Shotgun Proteome Analysis. Springer Theses. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-42008-5_1

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