Abstract
Optical resonator-based biosensors are emerging as one of the most sensitive microsystem biodetection technology that boasts all of the capabilities for a next-generation lab-on-chip device: label-free detection down to single molecules, multiplexed sensing capability, operation in aqueous environment as well as cost-effective integration on microchips. A scholarly introduction to the emerging field of whispering gallery mode resonator-based biosensors is given and their current applications are reviewed.
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References
J.L. Arlett et al., Comparative advantages of mechanical biosensors. Nat. Nanotechnol. 6, 203–215 (2011)
P.S. Waggoner, H.G. Craighead, Micro- and nanomechanical sensors for environmental, chemical, and biological detection. Lab Chip 7, 1238–1255 (2007)
F. Patolsky et al., Nanowire sensors for medicine and the life sciences. Nanomedicine 1, 51–65 (2006)
J. Li et al., Carbon nanotube sensors for gas and organic vapor detection. Nano Lett. 3, 929–933 (2003)
F. Vollmer, S. Arnold, Whispering-gallery-mode biosensing: label-free detection down to single molecules. Nat. Methods 5, 591–596 (2008)
P. Alivisatos, The use of nanocrystals in biological detection. Nat. Biotechnol. 22, 47–52 (2004)
J.N. Anker et al., Biosensing with plasmonic nanosensors. Nat. Mater. 7, 442–453 (2008)
K.K. Jain, Nanotechnology in clinical laboratory diagnostics. Clin. Chim. Acta 358, 37–54 (2005)
G.M. Whitesides, The ‘right’ size in nanobiotechnology. Nat. Biotechnol. 21, 1161–1165 (2003)
F. Vollmer et al., Single virus detection from the reactive shift of a whispering-gallery mode. Proc. Natl. Acad. Sci. USA 105, 20701–20704 (2008)
A.K. Naik et al., Towards single-molecule nanomechanical mass spectrometry. Nat. Nanotechnol. 4, 445–450 (2009)
M. Baaske, F. Vollmer, Optical resonator biosensors: molecular diagnostic and nanoparticle detection on an integrated platform. ChemPhysChem 13, 427–436 (2012)
F. Patolsky et al., Electrical detection of single viruses. Proc. Natl. Acad. Sci. USA 101, 14017–14022 (2004)
K. Wilson, F. Vollmer, Whispering Gallery Mode Resonator Biosensors, vol. 4 (Springer, Berlin, 2012)
F. Vollmer et al., Protein detection by optical shift of a resonant microcavity. Appl. Phys. Lett. 80, 4057–4059 (2002)
S. Arnold et al., Shift of whispering-gallery modes in microspheres by protein adsorption. Opt. Lett. 28, 272–274 (2003)
T.P. Burg et al., Weighing of biomolecules, single cells and single nanoparticles in fluid. Nature 446, 1066–1069 (2007)
F. Patolsky et al., Fabrication of silicon nanowire devices for ultrasensitive, label-free, real-time detection of biological and chemical species. Nat. Protoc. 1, 1711–1724 (2006)
H.K. Hunt et al., Bioconjugation strategies for microtoroidal optical resonators. Sensors 10, 9317–9336 (2010)
A.L. Washburn, R.C. Bailey, Photonics-on-a-chip: recent advances in integrated waveguides as enabling detection elements for real-world, lab-on-a-chip biosensing applications. Analyst 136, 227–236 (2011)
M. Curreli et al., Real-time, label-free detection of biological entities using nanowire-based FETs. IEEE Trans. Nanotechnol. 7, 651–667 (2008)
B. Ilic et al., Virus detection using nanoelectromechanical devices. Appl. Phys. Lett. 85, 2604–2606 (2004)
X.D. Fan, I.M. White, Optofluidic microsystems for chemical and biological analysis. Nat. Photonics 5, 591–597 (2011)
A.J. Qavi et al., Label-free technologies for quantitative multiparameter biological analysis. Anal. Bioanal. Chem. 394, 121–135 (2009)
M. Iqbal et al., Label-free biosensor arrays based on silicon ring resonators and high-speed optical scanning instrumentation. IEEE J. Sel. Top. Quantum Electron. 16, 654–661 (2010)
K. Vahala (ed.), Optical Microcavities. Advanced Series in Applied Physics (World Scientific, Hackensack, 2004)
A.J. Qavi, R.C. Bailey, Multiplexed detection and label-free quantitation of MicroRNAs using arrays of silicon photonic microring resonators. Angew. Chem., Int. Ed. Engl. 49, 4608–4611 (2010)
A. Ramachandran et al., A universal biosensing platform based on optical micro-ring resonators. Biosens. Bioelectron. 23, 939–944 (2008)
Y.Z. Sun, X.D. Fan, Optical ring resonators for biochemical and chemical sensing. Anal. Bioanal. Chem. 399, 205–211 (2011)
C.A. Barrios, Integrated microring resonator sensor arrays for labs-on-chips. Anal. Bioanal. Chem. 403, 1467–1475 (2012)
L.N. He et al., Ultrasensitive detection of mode splitting in active optical microcavities. Phys. Rev. A 82 (2010)
T. Lu et al., High sensitivity nanoparticle detection using optical microcavities. Proc. Natl. Acad. Sci. USA 108, 5976–5979 (2011)
Y. Lan et al., A self-reference sensing technique for ultra-sensitive chemical and biological detection using whispering gallery microresonators, Proc. SPIE Int. Soc. Opt. Eng. 7913, 791312 (2011)
J.G. Zhu et al., Single virus and nanoparticle size spectrometry by whispering-gallery-mode microcavities. Opt. Express 19, 16195–16206 (2011)
C. Shi et al., Leveraging bimodal kinetics to improve detection specificity. Opt. Lett. 37, 1643–1645 (2012)
I.M. White et al., Liquid-core optical ring-resonator sensors. Opt. Lett. 31, 1319–1321 (2006)
S.M. Harazim et al., Lab-in-a-tube: on-chip integration of glass optofluidic ring resonators for label-free sensing applications. Lab Chip 12, 2649–2655 (2012)
J.D. Suter et al., Label-free quantitative DNA detection using the liquid core optical ring resonator. Biosens. Bioelectron. 23, 1003–1009 (2008)
H.Y. Zhu et al., Analysis of biomolecule detection with optofluidic ring resonator sensors. Opt. Express 15, 9139–9146 (2007)
J. Lutti et al., A monolithic optical sensor based on whispering-gallery modes in polystyrene microspheres. Appl. Phys. Lett. 93 (2008)
F. Vollmer, Resonant detection of micro to nanoscopic OBjects using whispering gallery modes. PhD Thesis (2004)
K. De Vos et al., Silicon-on-insulator microring resonator for sensitive and label-free biosensing. Opt. Express 15, 7610–7615 (2007)
L. Jin et al., Highly-sensitive silicon-on-insulator sensor based on two cascaded micro-ring resonators with vernier effect. Opt. Commun. 284, 156–159 (2011)
M.S. Luchansky, R.C. Bailey, Silicon photonic microring resonators for quantitative cytokine detection and T-cell secretion analysis. Anal. Chem. 82, 1975–1981 (2010)
M.S. Luchansky, R.C. Bailey, Rapid, multiparameter profiling of cellular secretion using silicon photonic microring resonator arrays. J. Am. Chem. Soc. 133, 20500–20506 (2011)
M.S. Luchansky et al., Sensitive on-chip detection of a protein biomarker in human serum and plasma over an extended dynamic range using silicon photonic microring resonators and sub-micron beads. Lab Chip 11, 2042–2044 (2011)
B.Q. Su et al., Compact silicon-on-insulator dual-microring resonator optimized for sensing. J. Lightwave Technol. 29, 1535–1541 (2011)
H.Y. Zhu et al., Integrated refractive index optical ring resonator detector for capillary electrophoresis. Anal. Chem. 79, 930–937 (2007)
I.M. White et al., SERS-based detection in an optofluidic ring resonator platform. Opt. Express 15, 17433–17442 (2007)
J.T. Gohring et al., Detection of HER2 breast cancer biomarker using the opto-fluidic ring resonator biosensor. Sens. Actuators B, Chem. 146, 226–230 (2010)
J.T. Gohring, X.D. Fan, Label free detection of CD4+ and CD8+T cells using the optofluidic ring resonator. Sensors 10, 5798–5808 (2010)
J.D. Suter et al., Label-free DNA methylation analysis using opto-fluidic ring resonators. Biosens. Bioelectron. 26, 1016–1020 (2010)
K.A. Wilson et al., Whispering gallery mode biosensor quantification of fibronectin adsorption kinetics onto alkylsilane monolayers and interpretation of resultant cellular response. Biomaterials 33, 225–236 (2012)
C.E. Soteropulos et al., Determination of binding kinetics using whispering gallery mode microcavities. Appl. Phys. Lett. 99 (2011)
N.M. Hanumegowda et al., Refractometric sensors based on microsphere resonators. Appl. Phys. Lett. 87 (2005)
I. Teraoka, S. Arnold, Enhancing the sensitivity of a whispering-gallery mode microsphere sensor by a high-refractive-index surface layer. J. Opt. Soc. Am. B, Opt. Phys. 23, 1434–1441 (2006)
H.C. Ren et al., High-Q microsphere biosensor—analysis for adsorption of rodlike bacteria. Opt. Express 15, 17410–17423 (2007)
S. Arnold et al., Whispering gallery mode carousel—a photonic mechanism for enhanced nanoparticle detection in biosensing. Opt. Express 17, 6230–6238 (2009)
V.S. Ilchenko et al., Pigtailing the high-Q microsphere cavity: a simple fiber coupler for optical whispering-gallery modes. Opt. Lett. 24, 723–725 (1999)
V.S. Ilchenko, A.B. Matsko, Optical resonators with whispering-gallery modes, part II: applications. IEEE J. Sel. Top. Quantum Electron. 12, 15–32 (2006)
L. He et al., Detecting single viruses and nanoparticles using whispering gallery microlasers. Nat. Nanotechnol. 6, 428–432 (2011)
A. Yalcin et al., Optical sensing of biomolecules using microring resonators. IEEE J. Sel. Top. Quantum Electron. 12, 148–155 (2006)
C.Y. Chao et al., Polymer microring resonators for biochemical sensing applications. IEEE J. Sel. Top. Quantum Electron. 12, 134–142 (2006)
A. Ksendzov, Y. Lin, Integrated optics ring-resonator sensors for protein detection. Opt. Lett. 30, 3344–3346 (2005)
C. Delezoide et al., Vertically coupled polymer microracetrack resonators for label-free biochemical sensors. IEEE Photonics Technol. Lett. 24, 270–272 (2012)
J.T. Kirk et al., Multiplexed inkjet functionalization of silicon photonic biosensors. Lab Chip 11, 1372–1377 (2011)
A. Francois, M. Himmelhaus, Whispering gallery mode biosensor operated in the stimulated emission regime. Appl. Phys. Lett. 94 (2009)
M. Himmelhaus et al., Optical sensors based on whispering gallery modes in fluorescent microbeads: response to specific interactions. Sensors 10, 6257–6274 (2010)
M. Himmelhaus, A. Francois, In-vitro sensing of biomechanical forces in live cells by a whispering gallery mode biosensor. Biosens. Bioelectron. 25, 418–427 (2009)
J. Yang, L.J. Guo, Optical sensors based on active microcavities. IEEE J. Sel. Top. Quantum Electron. 12, 143–147 (2006)
A. Weller et al., Whispering gallery mode biosensors in the low-Q limit. Appl. Phys. B, Lasers Opt. 90, 561–567 (2008)
E. Nuhiji, P. Mulvaney, Detection of unlabeled oligonucleotide targets using whispering gallery modes in single, fluorescent microspheres. Small 3, 1408–1414 (2007)
A. Schweinsberg et al., An environmental sensor based on an integrated optical whispering gallery mode disk resonator. Sens. Actuators B, Chem. 123, 727–732 (2007)
R.W. Boyd, J.E. Heebner, Sensitive disk resonator photonic biosensor. Appl. Opt. 40, 5742–5747 (2001)
M. Pollinger et al., Ultrahigh-Q tunable whispering-gallery-mode microresonator. Phys. Rev. Lett. 103 (2009)
E.J. Smith et al., Lab-in-a-tube: detection of individual mouse cells for analysis in flexible split-wall microtube resonator sensors. Nano Lett. 11, 4037–4042 (2011)
J.D. Swaim et al., Detection limits in whispering gallery biosensors with plasmonic enhancement. Appl. Phys. Lett. 99 (2011)
M.A. Santiago-Cordoba et al., Nanoparticle-based protein detection by optical shift of a resonant microcavity. Appl. Phys. Lett. 99 (2011)
S.I. Shopova et al., Plasmonic enhancement of a whispering-gallery-mode biosensor for single nanoparticle detection. Appl. Phys. Lett. 98 (2011)
M.A. Santiago-Cordoba et al., Ultrasensitive detection of a protein by optical trapping in a photonic-plasmonic microcavity. J. Biophotonics 5, 629–638 (2012)
M.L. Gorodetsky et al., Ultimate Q of optical microsphere resonators. Opt. Lett. 21, 453–455 (1996)
J. Topolancik, F. Vollmer, Photoinduced transformations in bacteriorhodopsin membrane monitored with optical microcavities. Biophys. J. 92, 2223–2229 (2007)
F. Vollmer et al., Multiplexed DNA quantification by spectroscopic shift of two microsphere cavities. Biophys. J. 85, 1974–1979 (2003)
A.J. Qavi et al., Anti-DNA:RNA antibodies and silicon photonic microring resonators: increased sensitivity for multiplexed microRNA detection. Anal. Chem. 83, 5949–5956 (2011)
A.L. Washburn et al., DNA-encoding to improve performance and allow parallel evaluation of the binding characteristics of multiple antibodies in a surface-bound immunoassay format. Anal. Chem. 83, 3572–3580 (2011)
J.G. Zhu et al., On-chip single nanoparticle detection and sizing by mode splitting in an ultrahigh-Q microresonator. Nat. Photonics 4, 46–49 (2010)
W. Kim et al., Observation and characterization of mode splitting in microsphere resonators in aquatic environment. Appl. Phys. Lett. 98 (2011)
D.S. Weiss et al., Splitting of high-Q mie modes induced by light backscattering in silica microspheres. Opt. Lett. 20, 1835–1837 (1995)
M.L. Gorodetsky et al., Rayleigh scattering in high-Q microspheres. J. Opt. Soc. Am. B, Opt. Phys. 17, 1051–1057 (2000)
A. Mazzei et al., Controlled coupling of counterpropagating whispering-gallery modes by a single Rayleigh scatterer: a classical problem in a quantum optical light. Phys. Rev. Lett. 99 (2007)
M. Borselli et al., Beyond the Rayleigh scattering limit in high-Q silicon microdisks: theory and experiment. Opt. Express 13, 1515–1530 (2005)
T.J. Kippenberg et al., Purcell-factor-enhanced scattering from Si nanocrystals in an optical microcavity. Phys. Rev. Lett. 103 (2009)
K. De Vos et al., Multiplexed antibody detection with an array of silicon-on-insulator microring resonators. IEEE Photonics J. 1, 225–235 (2009)
H.A. Huckabay, R.C. Dunn, Whispering gallery mode imaging for the multiplexed detection of biomarkers. Sens. Actuators B, Chem. 160, 1262–1267 (2011)
S.Y. Lin, K.B. Crozier, Planar silicon microrings as wavelength-multiplexed optical traps for storing and sensing particles. Lab Chip 11, 4047–4051 (2011)
A.L. Washburn et al., Quantitative, label-free detection of five protein biomarkers using multiplexed arrays of silicon photonic microring resonators. Anal. Chem. 82, 69–72 (2010)
O. Scheler et al., Label-free, multiplexed detection of bacterial tmRNA using silicon photonic microring resonators. Biosens. Bioelectron. 36, 56–61 (2012)
M.S. McClellan et al., Label-free virus detection using silicon photonic microring resonators. Biosens. Bioelectron. 31, 388–392 (2012)
V.R. Dantham et al., Taking whispering gallery-mode single virus detection and sizing to the limit. Appl. Phys. Lett. 101 (2012)
F. Vollmer, L. Yang, Label-free detection with high-Q microcavities: a review of biosensing mechanisms for integrated devices, in Nanophotonics (de Gruyter, Berlin, 2012). doi:10.1515/nanoph-2012-0021
M.R. Foreman, F. Vollmer, Level repulsion in hybrid photonic-plasmonic microresonators for enhanced biodetection. Phys. Rev. A 88 (2013)
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Wu, Y., Vollmer, F. (2014). Whispering Gallery Mode Biomolecular Sensors. In: Gagliardi, G., Loock, HP. (eds) Cavity-Enhanced Spectroscopy and Sensing. Springer Series in Optical Sciences, vol 179. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-40003-2_9
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DOI: https://doi.org/10.1007/978-3-642-40003-2_9
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