Advertisement

Study of Glass Micropipettes from Tip Formation to Characterization

  • Majid MalboubiEmail author
  • Kyle Jiang
Chapter
Part of the SpringerBriefs in Applied Sciences and Technology book series (BRIEFSAPPLSCIENCES)

Abstract

In this chapter various aspects of glass micropipettes are studied, including mechanisms of tip formation, tip geometry, and effect of pulling parameters on surface roughness properties of glass micropipettes.

Keywords

Glass Micropipette Sutter Instrument Roundness Error Fitted Circle Seal Formation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Bruckbauer A et al (2007) Nanopipette delivery of individual molecules to cellular compartments for single-molecule fluorescence tracking. J Biophys 93:3120–3131Google Scholar
  2. 2.
    Keith Martin RG, Klein RL, Quigley HA (2002) Gene delivery to the eye using adeno-associated viral vectors. Methods 28:267–275Google Scholar
  3. 3.
    Kimura Y, Yanagimachi R (1995) Intracytoplasmic sperm injection in the mouse. Biol Reprod 52:709–720Google Scholar
  4. 4.
    Yaul M, Bhatti R, Lawrence, S (2008) Evaluating the process of polishing borosilicate glass capillaries used for fabrication of in vitro fertilization (iVF) micro-pipettes. Biomed Microdevices 10:123–128Google Scholar
  5. 5.
    Brown KT, Flaming DG (1995) Advanced Micropipette Techniques for Cell Physiology. John Wiley & Sons, San FranciscoGoogle Scholar
  6. 6.
    Neher E, Sakmann B (1976) Single-channel currents recorded from membrane of denervated frog muscle fibres. Nature 260:799–802Google Scholar
  7. 7.
    Huebner A et al (2008) Microdroplets: a sea of applications?. Lab Chip 8:1244–1254Google Scholar
  8. 8.
    Hong M H et al (2000) Scanning nanolithography using a material-filled nanopipette. Appl Phys lett 77:2604–2606Google Scholar
  9. 9.
    Ying L et al (2005) The scanned nanopipette: a new tool for high resolution bioimaging and controlled deposition of biomolecules. Phys Chem Chem Phys 7:2859–2866Google Scholar
  10. 10.
    Operational Manual P-97 Flaming/Brown Micropipette Puller Sutter instrument company. [Online] www.sutter.com
  11. 11.
    Flaming Dale G (1986) Method of forming an ultrafine micropipette. CA 4600424, 15-July 1986Google Scholar
  12. 12.
    Oesterle A (2009) Personal communication. Sutter instruments, 24 Jan 2009Google Scholar
  13. 13.
    Marinello F et al (2008) Critical factors in SEM 3D stereomicroscopy. Meas Sci Technol 19:1–12Google Scholar
  14. 14.
    Malboubi M, Gu Y, Jiang K (2011) Surface properties of glass micropipettes and their effect on biological studies. Nanoscale Res Lett 6:1–10Google Scholar
  15. 15.
    Lepple-Wienhues A et al (2003) Flip the tip: an automated, high quality, cost-effective patch clamp screen. Receptors Channels 9:13–17Google Scholar
  16. 16.
    Kubis AJ et al (2004) Focused-ion beam tomography. Metall Mater Trans A 35(7):1935–1943Google Scholar
  17. 17.
    Canny J (1986) A computational approach to edge detection. IEEE Trans Pattern Anal Mach Intell 8:679–714Google Scholar
  18. 18.
    Gander W, Golub GH, Strebel R (1994) Least-squares fitting of circles and ellipses. BIT Numer Math 34:558–578Google Scholar
  19. 19.
    Purves Robert D (1980 March) The mechanics of pulling a glass micropipette. Biophys J 29:523–530Google Scholar

Copyright information

© The Author(s) 2014

Authors and Affiliations

  1. 1.London Centre for NanotechnologyLondonUK
  2. 2.School of Mechanical EngineeringThe University of BirminghamBirminghamUK

Personalised recommendations