Nucleic Acid Sequencing and Analysis with Nanopores

  • Slaven GarajEmail author
Part of the Nucleic Acids and Molecular Biology book series (NUCLEIC, volume 29)


It has recently been recognized that solid-state nanopores in single-atomic-layer graphene membranes can be used to electronically detect and characterize single long charged polymer molecules. We have now fabricated nanopores in single-layer graphene that are closely matched to the diameter of a double-stranded DNA molecule. Ionic current signals during electrophoretically driven translocation of DNA through these nanopores were experimentally explored and theoretically modeled. Our experiments show that these nanopores have unusually high sensitivity (0.65 nA/Å) to extremely small changes in the translocating molecule’s outer diameter. Such atomically short graphene nanopores can also resolve nanoscale-spaced molecular structures along the length of a polymer, but they do so with greatest sensitivity only when the pore and molecule diameters are closely matched. Modeling confirms that our most closely matched pores have an inherent resolution of ≤0.6 nm along the length of the molecule.


Graphene Membrane Longitudinal Resolution Current Blockade Graphene Nanopores Ionic Current Signal 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We would like to acknowledge financial support from the Singapore National Research Foundation (grant NRF-NRFFOO1-131).


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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.Department of PhysicsNational University of SingaporeSingaporeSingapore
  2. 2.Department of BioengineeringNational University of SingaporeSingaporeSingapore
  3. 3.Graphene Research CenterNational University of SingaporeSingaporeSingapore
  4. 4.Nanoscience & Nanotechnology Initiative (NUSNNI)National University of SingaporeSingaporeSingapore

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