Abstract
The effects of different concentration of ethanol on enzymatic activity, thermal stability, heavy metallic ion stability, and pH stability of Δ1-dehydrogenase from Arthrobacter simplex were investigated in this paper. The results showed that the introduction of no more than 6 % (v/v) of ethanol had obvious effect on pH of culture and Δ1-dehydrogenase catalytic activity, respectively. The activation energy of deactivation (E a ) had a slight increase after the cell was treated by 2 % (v/v) of ethanol, which indicated that the addition of ethanol (2 %, v/v) could enhance the thermal stability of Δ1-dehydrogenase. The tendency of value of half-life (t 1/2) with the content of ethanol was similar in the different concentrations of Ba2+. The maximal value of t 1/2 was observed at 2 % of ethanol and then drastically decreased with the increase of ethanol. The t 1/2 value of Δ1-dehydrogenase was obviously decreased with the increase of ethanol concentration in the buffers with the initial pH 7.0 and 10.0, respectively.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Alexander DL, Fisher JE (1995) Advances in microbial steroid biotransformation. Steroids 60:290–294
Fernandes P, Cruz A, Angelova B, Pinheiro HM, Cabral JMS (2003) Microbial conversion of steroid compounds: recent developments. Enzyme Microb Technol 32:688–705
Simon LM, Lásló K (1998) Effects of pH value and Zn2+ concentration on bioactivity and structure of trypsin. J Mol Catal B Enzym 4:41–45
Smith M, Zahnley J (1993) Growth and cholesterol oxidation by mycobacterium species in Tween 80 medium. Appl Environ Microb 59:1425–1429
Bie ST, Lu FP (2008) Effect of phase composition on the bioconversion of methyltestosterone in a biphasic system. J Mol Catal B Enzym 55(1–2):1–5
Shen YB, Wang M, Li HN, Wang YB, Luo JM (2012) Influence of hydroxypropyl-β-cyclodextrin on phytosterol biotransformation by different strains of mycobacterium neoaurum. Ind Microb Biotechnol 39:1253–1259
Wang ZL, Zhao FS (2004) Microbial transformation of hydrophobic compound in cloud point system. J Mol Catal B Enzym 27(4–6):147–153
Griebenow K, Laureano Y (1999) Effect of prolonged exposure to organic solvents on the active site environment of subtilisin carlsberg. J Am Chem Soc 121:8157–8163
Rantwijk FV, Lau RM (2003) Biocatalytic transformations in ionic liquids. Trends Biotechnol 21:131–138
Zheng SX, Wang RZ, Xie SL (2011) Research progress in biodehydrogenation technique of steroid medicine. Chin J Biochem Pharm 32(3):251–254
Xue JH, Li H, Wei P (2012) Microbial metabolomics and its application in industry. Biotechnol Bull 2:20–27
Li SY, Ma YQ, Su YL (2012) Effect of heavy metal stress on microbial growth. Guizhou Agric Sci 40(2):90–94
Chen YG, Chen XM, Zhang YG (2009) The physiological mechanism of microbial heavy metals tolerance. Biotechnol Bull 10:60–65
Wang JF, Luo JM, Song Y (2011) The effects of ethanol on cell growth and cell characteristics of Arthrobacter simplex TCCC 11037. Chem Bioeng 28(3):66–69
Luo JM, Song Y, Wang JF, Ning J, Cheng YX (2012) Study on the effects of different kinds of organic solvents on C1,2 dehydrogenation reaction by the analysis of Arthrobacter simplex cell characters. Chem Bioeng 29(5):49–53
Zhang LT, Wang M, Shen YB, Ma YH, Luo JM (2009) Improvement of steroid biotransformation with hydroxypropyl-β-cyclodextrin induced complexation. Appl Biochem Biotechnol 3(159):642–654
Choi KP, Molnár I, Yamashita M, Murooka Y (1995) Purification and characterization of the 3-ketosteroid-delta 1-dehydrogenase of Arthrobacter simplex produced in Streptomyces lividans. Mol Microbiol 117(5):1043–1049
Acknowledgments
This work is supported by Scientific Research Foundation Project of Tianjin University of Science and Technology (No. 20100210) and Lab Opening Foundation of Tianjin University of Science and Technology (No. 1204A210).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Luo, J. et al. (2014). The Effect of Ethanol on Steroid Δ1-dehydrogenase from Arthrobacter simplex . In: Zhang, TC., Ouyang, P., Kaplan, S., Skarnes, B. (eds) Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Lecture Notes in Electrical Engineering, vol 251. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-37925-3_192
Download citation
DOI: https://doi.org/10.1007/978-3-642-37925-3_192
Published:
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-37924-6
Online ISBN: 978-3-642-37925-3
eBook Packages: EngineeringEngineering (R0)