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Identification of an Antifungal Bacillus Strain and Studies on Its Antifungal Active Ingredients

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Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012)

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 251))

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Abstract

Strain NK-2 which has broad spectrum of antifungal activity against eight kinds of phytopathogenic fungi was identified as Bacillus amyloliquefaciens by analysis of partial 16S rDNA, gyrA gene, and rpoB gene sequences. The crude protein, obtained from NK-2 culture, exhibited inhibitory activity on mycelial growth and conidial spore germination of Fusarium oxysporum. The crude proteins were further purified by Sephadex G-200 and the antifungal fraction was checked by SDS-PAGE. The putative active protein was then analyzed by Tandem mass spectrum. The result revealed that the target protein was high similar to protein YhfE from B. amyloliquefaciens FZB 42. Then we cloned the yhfE (GenBank accession:HM143898) from NK-2 genome and expressed it in Escherichia coli BL21. The purified recombinant YhfE protein could also inhibit the spore germination and mycelial growth of F. oxysporum. We have found for the first time, to our knowledge, that YhfE has antifungal activity.

The authors Y. Shen and R. Liu contributed equally to this paper.

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Acknowledgments

This work was supported by the Development Program of China (863 Program) (No. 2011AA10A203), the National Natural Science Foundation of China grant (30971957), and Natural Science Foundation of Tianjin Science and Technology Commission (11JCYBJC08300, 12JCYBJC19800).

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Authors and Affiliations

  1. Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Science, Nankai University, Tianjin, 300071, People’s Republic of China

    Yingde Shen, Yuehua Chen & Jun Cai

  2. Tianjin Taihe Pharmaceutical Co. Ltd., Tianjin, 300270, People’s Republic of China

    Ruibin Liu

Authors
  1. Yingde Shen
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  2. Ruibin Liu
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  3. Yuehua Chen
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  4. Jun Cai
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Corresponding author

Correspondence to Jun Cai .

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Editors and Affiliations

  1. College of Bioengineering, Tianjin University of Science and Technology, Tianjin, People's Republic of China

    Tong-Cun Zhang

  2. Nanjing University of Technology, Nanjing, People's Republic of China

    Pingkai Ouyang

  3. Department of Microbiology & Molecular Genetics, University of Texas- Houston Medical School, Texas, Texas, USA

    Samuel Kaplan

  4. Wellcome Trust Sanger Institute, Cambridge, United Kingdom

    Bill Skarnes

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Shen, Y., Liu, R., Chen, Y., Cai, J. (2014). Identification of an Antifungal Bacillus Strain and Studies on Its Antifungal Active Ingredients. In: Zhang, TC., Ouyang, P., Kaplan, S., Skarnes, B. (eds) Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Lecture Notes in Electrical Engineering, vol 251. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-37925-3_175

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  • DOI: https://doi.org/10.1007/978-3-642-37925-3_175

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-37924-6

  • Online ISBN: 978-3-642-37925-3

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